Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	2,708,473	T→G	27.8%	V44G (GTG→GGG)	CJ019_02558 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	2,708,473	0	T	G	27.8%	44.6 / 2.1	48	V44G (GTG→GGG)	CJ019_02558	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (15/18);  new base G (3/12);  total (18/30)
Fisher's exact test for biased strand distribution p-value = 1.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACG  >  ACB122/2708344‑2708597                                                                                                                                  |                                                                                                                             tGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCt                                                                                                                         <  7:171226/135‑1 (MQ=255)      cATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCGATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGGGGGCTGcccc                                                                                                                    >  6:351574/1‑133 (MQ=255)                     ggCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTTTTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGGGGCCCGCCCCGCCCGCCaccccccc                                                                                                     >  5:74694/1‑129 (MQ=255)                         ccTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCACCCGATCTTCACCGAGAAGTGCGTGGCCGGCCCCGCCGGCCACCCCgccgcca                                                                                                 >  7:35199/1‑134 (MQ=255)                          cTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCCCCGAGAAGTGCGTGGCCGGCCCCGCCTGCCACGCCgcccccgg                                                                                                >  4:135496/1‑133 (MQ=255)                          cTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGGCCCGCCGGCAACGACGCCGCCTg                                                                                                >  8:125202/1‑135 (MQ=255)                              gCCCTGCTCATCCGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGCGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGGGGCCTGCCACGCCTGCAACGACGCCGCCTGCCa                                                                                             <  1:357214/134‑1 (MQ=255)                                       atcaGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCGGGAACGACGCCGCCTGCCAGCGGAAGCTg                                                                                   >  2:46540/1‑135 (MQ=255)                                        tcaGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACCCCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCt                                                                                    <  7:300559/133‑1 (MQ=255)                                        tcaGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCt                                                                                    <  7:56465/133‑1 (MQ=255)                                               cgcgGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCGGCCCCCCCGGCAACGCCCCCGCCTGCCAGCGGAAGCAggtacgc                                                                             >  3:173833/1‑128 (MQ=255)                                                  tgTATTCGGCCAGGCCCCCCTGGCGAGCCCGGCTATTTCCTACACCCGGGAAATTCAACCGATCTTCACCGATAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGa                                                                        <  7:324348/134‑1 (MQ=255)                                                            cAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGCCATTCAACCGATCTGCACCGAGAAGTGCGGGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTa                                                              <  2:23270/135‑1 (MQ=255)                                                               gCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTAcgc                                                           <  6:87382/135‑1 (MQ=255)                                                                 cccccAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTTGCCTGCCCCGCCGGCCACGCCGCCGCCTGCCAGCTCAAGCTGGAAAGCCCGGAAGGCGCGGTACGCgg                                                         >  7:65717/1‑135 (MQ=255)                                                                 cccccAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCCCGCCGGCAACGCCGCCGCCTGCCAGCGCAAGCGGGAAAGCCCGGAAGGCGCGGTACGCgg                                                         >  7:296364/1‑135 (MQ=255)                                                                 cccccAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAAACGATCTTCACCGAGAAGTGCGGGGCCGGCCACGCCGGCCAAGACGCCGCCTTCCCTCGCACGCTGGAACGCCCTGACGGCGCGGTCCGCgg                                                         >  6:229138/1‑135 (MQ=255)                                                                   cccAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCgg                                                         >  4:280222/1‑133 (MQ=255)                                                                   cccAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCgg                                                         <  3:280222/133‑1 (MQ=255)                                                                      cgccgcgCCGGGCTAGTCCCACCACCCGGCACATCCACCCGACCTCCCCGCACACGGGCGGGGCCGCCCACGCCGGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCa                                                    <  5:212744/129‑1 (MQ=255)                                                                          gtgCCCGGCTATTTCCTACACCGGGGACTTTCACCGTATCTTCACGAAGACGTGCGGGGCGTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGGGCGGTACGCGGGGCCAGCaa                                                <  3:313505/133‑1 (MQ=255)                                                                            gCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAgg                                              <  4:326481/135‑1 (MQ=255)                                                                              cctgCTCTTTCCTACCCCCGGGCCAGGCACCCGCTCTTCCCCGAGCAGTGCGTTGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTTGAAAGCCCGGAAGGCGCGGTACGCGGGGCCAGCAAgggc                                            <  5:229138/132‑3 (MQ=255)                                                                              ccGGCTATTTCCTACACCCGGTACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTc                                            <  2:66566/135‑1 (MQ=255)                                                                              ccGGATATTTCCTACACCCGGCACATTCAACCGATCTTCTCCGAGAAGTGCGGGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAATCTGGAAAGCCCTTAAGGCGCGGTACGCGGGTCCAGCAAGGTc                                            <  3:274959/135‑1 (MQ=255)                                                                               cGGCTATTTCCTCCACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGGGGCCTGCCACGCCGGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTcc                                           <  5:171967/135‑1 (MQ=255)                                                                                ggCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGGGTGGGCTGGCCCCCCGGCCACGCCCCCGCCGGCCCCCGCAAGCTGGAAAGCCCAGAATGCGCGGGGCGCGGGGCCCGCAAGGTccc                                          >  5:203826/1‑135 (MQ=255)                                                                                ggCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCGGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTccc                                          >  7:274307/1‑135 (MQ=255)                                                                                  cTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCCCGCCTGCCACGACGCCGCCTGCCCGCGCAAGCTGGAAAGCCCTGAAGGCGCGGGACGCGGGGGCCGCAAGGTCCCgg                                        >  4:318875/1‑135 (MQ=255)                                                                                             acCCGGTACATTCACCCGATCTTCACCGACAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGc                             <  5:293091/135‑1 (MQ=255)                                                                                             acCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGc                             <  3:371460/135‑1 (MQ=255)                                                                                             acCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGGGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGc                             <  7:83748/135‑1 (MQ=255)                                                                                             acCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGGGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGc                             <  6:95304/135‑1 (MQ=255)                                                                                             acCCGGGACATTCAACCGATCTTCACCGAGAAGGGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGc                             <  8:21110/135‑1 (MQ=255)                                                                                             acCCGGGACATTCAACCGATCTTCACCGAGAAGGGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGc                             <  6:120576/135‑1 (MQ=255)                                                                                             acCCGGGACATTCAACCGAGCTTCACCGAGAAGTGCGGGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGc                             <  3:318875/135‑1 (MQ=255)                                                                                             acCCGGCACATTCAACCGATCTTCACCGAGAAGTGCGGGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGGCCCGGTGTACCAGGGc                             <  4:251404/135‑1 (MQ=255)                                                                                             acCCGGCACATGCAACCGATCTTCACCGAGATGTGCGGGCCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGc                             <  1:351822/135‑1 (MQ=255)                                                                                               ccGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGa                           <  6:74694/135‑1 (MQ=255)                                                                                               ccGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCAGGAACCACGCCGCCTGCCCGCTCCAGCTGGGAAGCCCAGCAGGCGCGGGACCCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCgc                           >  6:243223/1‑134 (MQ=255)                                                                                                  ggACATTCAACCGATCTTCACCGAGAAGGGCGTGGCCGGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCg                        <  6:214709/135‑1 (MQ=255)                                                                                                     cATTCAACCGATCTTCACCGAGAAGTGCGTGGCCGGCCCCGCCCGCCACCCCGCCGCCAGCCAGCGCAAACTGGCAAGCCCGGAACGCGCCGTACGCCGGGCCCGCACAGTCCCGGTGTACCAGGGCGCGCGGAg                     >  8:199227/1‑135 (MQ=255)                                                                                                       ttCAACCGATCTTCACCGAGACGTGCGGGGCCGGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCa                   <  2:324061/135‑1 (MQ=255)                                                                                                           aCCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGCAAGCCCTGAAGGCGCGGTACGCGGGGCCCGCAAGGTCCCGGTGTACCAGGGCGCGCGGAGCAAg                 >  3:138987/1‑133 (MQ=255)                                                                                                                  ttCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCa                   >  6:80253/1‑124 (MQ=255)                                                                                                                  ttCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCa                   <  5:80253/124‑1 (MQ=255)                                                                                                                        gagaAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCAcg  <  3:111088/135‑1 (MQ=255)                                                                                                                        gagaAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGGCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCAcg  <  8:340715/135‑1 (MQ=255)                                                                                                                                  |                                                                                                                             TGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACG  >  ACB122/2708344‑2708597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑