Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,827,559 C→A 6.3% V302F (GTT→TTT)  aldA_2 ← Putative aldehyde dehydrogenase AldA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,827,5590CA 6.3% 198.2 / 3.8 64V302F (GTT→TTT) aldA_2Putative aldehyde dehydrogenase AldA
Reads supporting (aligned to +/- strand):  ref base C (28/32);  new base A (2/2);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

AGGATCCGTTCCATCTGGGTCTTGCTGACCTGTGCGCCCATCATGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTGCCGCCCAGTTCAAGGG  >  ACB122/2827426‑2827690
                                                                                                                                     |                                                                                                                                   
aGGATCCGTTCCATCTGGGTCTTGCTGACCTGTGCGCCCATCATGGTGTCCGGGTTCAATGGGTCACACACACGCACAGCTTCGACCTTATGCTTGAGCTCAGCCAGGCATCCCTCGTAGATGGATTCGTgaac                                                                                                                                     >  2:161084/1‑134 (MQ=255)
   aTCCGTTCCATCTGGGTCTTGCTGACCTGTGCGCCCATCATGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTgaacga                                                                                                                                   >  2:114800/1‑133 (MQ=255)
           cATCTGGGTCTTGCTGACCTGTGCGCCCATCATGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAg                                                                                                                          <  7:186277/134‑1 (MQ=255)
                gggTCTTGCTGACCTGTGCGCCCATCATGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCgg                                                                                                                    >  8:70167/1‑135 (MQ=255)
                     ttGCTGACCTGTGCGCCCATCATGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCg                                                                                                               >  3:240874/1‑135 (MQ=255)
                         tGACCTGTGCGCCCATCATGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGa                                                                                                           >  4:367122/1‑135 (MQ=255)
                              tgtgCGCCCATCATGGTGTCCGTGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTgg                                                                                                      <  3:158470/135‑1 (MQ=255)
                                 gcgcCCCTCATGGTGTCCGGGGTCAATGGGTCACCCACACGCCCAGCTTCGAACTTATGCTTGAGCGCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGc                                                                                                     <  6:20096/133‑1 (MQ=255)
                                   gcCCATCATGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTg                                                                                                 >  5:381879/1‑135 (MQ=255)
                                   gcCCATCATGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTg                                                                                                 >  6:149270/1‑135 (MQ=255)
                                   gcCCATCATGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTg                                                                                                 >  3:63382/1‑135 (MQ=255)
                                   gcCCATCATGGTGTCCGGGTTC‑ATGGGTCTCCCCCACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTgg                                                                                                >  7:15715/1‑135 (MQ=255)
                                      catcatGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGtt                                                                                              <  4:235373/135‑1 (MQ=255)
                                          gactggtCCGGGTTCAATGGGTCACGCTCACGCTCAGCTTCGAACTTAAGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCcac                                                                                          <  4:172960/130‑1 (MQ=255)
                                           tGGTGTCCGGGTTCAATGTGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCcac                                                                                          <  1:46155/134‑1 (MQ=255)
                                           tGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCcac                                                                                          <  4:88003/134‑1 (MQ=255)
                                           tGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCcac                                                                                          <  2:102646/134‑1 (MQ=255)
                                           tGGTGTCCGGGATCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCcac                                                                                          <  7:324003/134‑1 (MQ=255)
                                           tGGGGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCcac                                                                                          <  1:135897/134‑1 (MQ=255)
                                               gtCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGAt                                                                                     <  7:70167/135‑1 (MQ=255)
                                               gtCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGAt                                                                                     <  2:158498/135‑1 (MQ=255)
                                               gtCCGGGTTCAATGGGGCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGAt                                                                                     <  6:362151/135‑1 (MQ=255)
                                                    ggTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGa                                                                                >  2:47362/1‑135 (MQ=255)
                                                     gTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGa                                                                                >  1:316345/1‑134 (MQ=255)
                                                         aaTGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACt                                                                           <  8:322364/135‑1 (MQ=255)
                                                          aTGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACt                                                                           <  7:50693/134‑1 (MQ=255)
                                                             ggTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTCGCCTTGGTTCCACAGGATGGCGAGGAcctgtc                                                                       >  3:172960/1‑130 (MQ=255)
                                                                cACCCACACGCACATCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCtt                                                                    <  3:181559/135‑1 (MQ=255)
                                                                cACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCtt                                                                    >  1:170097/1‑135 (MQ=255)
                                                                cACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCtt                                                                    <  1:277410/135‑1 (MQ=255)
                                                                 aCCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCtt                                                                    >  1:294507/1‑134 (MQ=255)
                                                                 aCCCACACGCACAGCTTCGAACTTATGCTTGAGCGCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTc                                                                   <  5:66259/135‑1 (MQ=255)
                                                                               cTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTc                                                       >  4:198093/1‑133 (MQ=255)
                                                                                ttCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCa                                                    >  7:43468/1‑135 (MQ=255)
                                                                                 tCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTACATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAg                                                   >  1:149668/1‑135 (MQ=255)
                                                                                  cGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGt                                                  >  8:98014/1‑135 (MQ=255)
                                                                                    aaCTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTc                                                <  5:221792/135‑1 (MQ=255)
                                                                                            cTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCggg                                        >  1:286876/1‑135 (MQ=255)
                                                                                            cTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCggg                                        >  1:149602/1‑135 (MQ=255)
                                                                                            cTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCgg                                         >  3:164568/1‑134 (MQ=255)
                                                                                            cTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCgg                                         >  4:367322/1‑134 (MQ=255)
                                                                                            cTTGAGCTCAGCCAGGAATCGATCGTAGATGGATTCGTGAACGAACAGCCGAGAGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCgg                                         >  1:7033/1‑134 (MQ=255)
                                                                                               gAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGa                                      <  1:47362/134‑1 (MQ=255)
                                                                                                  cTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAAc                                  >  1:138804/1‑135 (MQ=255)
                                                                                                          ggAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTgg                          <  6:125714/135‑1 (MQ=255)
                                                                                                               cGCTCGGAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATGCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGAc                     <  4:58626/135‑1 (MQ=255)
                                                                                                               cGCTCGCAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGAc                     <  4:58623/135‑1 (MQ=255)
                                                                                                                  tCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTg                  <  2:80362/135‑1 (MQ=255)
                                                                                                                  tCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTg                  >  6:249298/1‑135 (MQ=255)
                                                                                                                  tCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGT‑CCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTgc                 >  5:334316/1‑135 (MQ=255)
                                                                                                                    gTAGATGGATTCGTGAACGCACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGACTTCCACGGCATAGTCCCAGATCGCATCTGGGAAAACGATCTTGGCCGACTAgcc                >  7:366074/1‑135 (MQ=255)
                                                                                                                    gTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACtt                                                                                                        <  3:298215/47‑1 (MQ=255)
                                                                                                                    gTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACtt                                                                                                        >  4:298215/1‑47 (MQ=255)
                                                                                                                    gTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTgcc                <  7:98014/135‑1 (MQ=255)
                                                                                                                    gTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTgcc                <  7:367552/135‑1 (MQ=255)
                                                                                                                     tAGATGGATTCGTGAACGAACAGCCGAGCGGCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTgcc                <  4:240874/134‑1 (MQ=255)
                                                                                                                     tAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTgcc                <  3:372937/134‑1 (MQ=255)
                                                                                                                     tAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTgcc                <  2:170097/134‑1 (MQ=255)
                                                                                                                     tAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTgcc                <  2:316345/134‑1 (MQ=255)
                                                                                                                     tAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTgcc                <  3:171099/134‑1 (MQ=255)
                                                                                                                         tGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTGCCGCCCa           >  4:207796/1‑135 (MQ=255)
                                                                                                                               cGTGAACGAACAGCCGAGCGCCGGATTCGCAGATTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTGCCGCCCAGTTCaa     >  2:253328/1‑135 (MQ=255)
                                                                                                                               cGTGAAAGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTc                                                                   <  2:30781/73‑1 (MQ=255)
                                                                                                                               cGTGAAAGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTc                                                                   >  1:37452/1‑73 (MQ=255)
                                                                                                                               cGTGAAAGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTc                                                                   >  1:30781/1‑73 (MQ=255)
                                                                                                                               cGTGAAAGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTc                                                                   <  2:37452/73‑1 (MQ=255)
                                                                                                                                  gaacgaacAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTGCCGCCCAGTTCAAggg  >  6:142093/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                   
AGGATCCGTTCCATCTGGGTCTTGCTGACCTGTGCGCCCATCATGGTGTCCGGGTTCAATGGGTCACCCACACGCACAGCTTCGAACTTATGCTTGAGCTCAGCCAGGAATCGCTCGTAGATGGATTCGTGAACGAACAGCCGAGCGCCGGATTCGCAGACTTGGCCTTGGTTCCACAGGATGGCGAGGACTGCGCCTTCCACGGCCTTGTCCCAGTTCGCATCGGGGAAAACGATGTTGGCCGACTTGCCGCCCAGTTCAAGGG  >  ACB122/2827426‑2827690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑