| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,194,920 | T→C | 6.6% | F397F (TTT→TTC) | puuB_3 → | Gamma‑glutamylputrescine oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,194,920 | 0 | T | C | 6.6% | 186.5 / 2.2 | 61 | F397F (TTT→TTC) | puuB_3 | Gamma‑glutamylputrescine oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (31/25); new base C (2/2); total (34/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTGCGGCGGCCAAGTTGCTGG > ACB122/3194786‑3195047 | tgGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGttt > 6:87669/1‑135 (MQ=255)tgGAGTCGTTACCTCAATGGCGCCTAAGACTTTATCCATGTTATCGGAGCCATCGGCGCGAAACAGAACATTTACTACGCCACCGCCAGCTCGGGCCACGGGCTGGCCACGCAATCGTTGATCGCCAATCTCttt > 6:301664/1‑135 (MQ=255) gcGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa > 3:268414/2‑135 (MQ=255) aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa > 8:21611/1‑135 (MQ=255) aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa > 3:274795/1‑135 (MQ=255) aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa > 4:109708/1‑135 (MQ=255) aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa > 6:193352/1‑135 (MQ=255) aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaaa > 1:194156/1‑135 (MQ=255) aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTCGCCCAGCaaa > 6:141661/1‑135 (MQ=255) aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACAATTACTACCCCACCGGCTGCGCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTAGCCCAGCaaa > 5:7814/1‑135 (MQ=255) aCGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCCGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCaa > 1:200031/1‑134 (MQ=255) aCGACAGTGCCGACAACGACTTTCTTCCGGTCATAGGTGCAATAGTAGACAAACAGAACATTTACTACGCCAACTGCTGATCTGTCCAAGGGCTTGGCACGCAATCGTTGATACTCAAGCTGTTTGCCCAGCaaa > 8:261544/1‑135 (MQ=255) aGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAGTCAAc > 7:208433/1‑135 (MQ=255) aGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAAc > 1:83557/1‑135 (MQ=255) ggCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGc > 2:137157/1‑135 (MQ=255) ggCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGc > 1:236884/1‑135 (MQ=255) cgcCTACGACTTTCTGCCGGTGATCGGTGCAATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGCGCCACGGGCTGGGCACGCAAACGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCa > 3:188641/1‑135 (MQ=255) cgcCTACGACGTTATGCCCGTGATCGGTGCCATAGGCGAGAAACAGAACAATTACTACGCCACCGGCTGCTACGGCCACGGGCAGGGACAGCAATCGTTCATCGGCAAGCTGTTTGCCCAGCAAATCAAAGGCCa > 8:254962/1‑135 (MQ=255) aCGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACAGTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGgcgc > 3:128385/1‑135 (MQ=255) cGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg < 7:21611/135‑1 (MQ=255) cGGTGATCGGTGCCAGGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAgctgc < 6:146705/132‑1 (MQ=255) cGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACt < 4:274795/135‑1 (MQ=255) ggggCCCTGGGCGCGAAACAGACCATTTCCTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTg < 1:152664/132‑1 (MQ=255) gcattGGGCGCGAACCAGAACTTTACCTCCGCCACCGGCTGCGCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCATCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCaa < 5:53955/131‑1 (MQ=255) gggCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACaa < 4:268414/135‑1 (MQ=255) gcgcGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACaaaa < 2:39242/135‑1 (MQ=255) cgcgAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAAc < 8:165713/135‑1 (MQ=255) aaaCAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCg > 1:49253/1‑135 (MQ=255) aaaCAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTCGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg > 6:14657/1‑112 (MQ=255) aaaCAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTCGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg < 5:14657/112‑1 (MQ=255) cAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTcc < 5:117318/135‑1 (MQ=255) cAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTcc < 4:72580/135‑1 (MQ=255) tttACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTCGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTtgctgc < 5:141661/135‑1 (MQ=255) ctacGCCACCGGCTGCTCGGGCCACTGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTg < 7:62244/134‑1 (MQ=255) ctacGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg > 4:45025/1‑98 (MQ=255) ctacGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg < 3:45025/98‑1 (MQ=255) ctacGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTg < 4:168713/134‑1 (MQ=255) ctacGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTg < 3:33709/134‑1 (MQ=255) tacGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCTAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGaa < 2:216131/135‑1 (MQ=255) acGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAAc > 5:298792/1‑135 (MQ=255) gCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAAccc < 1:10475/135‑1 (MQ=255) ccACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGTCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCt > 8:231256/1‑135 (MQ=255) ccACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACCCCGTCCTTGCTGCCTGAACCCt > 8:225550/1‑135 (MQ=255) ccGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg > 8:277021/1‑90 (MQ=255) ccGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTgg < 7:277021/90‑1 (MQ=255) ccGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTg > 7:160944/1‑134 (MQ=255) ccGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTg > 6:145355/1‑134 (MQ=255) cGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAAccc > 2:338818/1‑130 (MQ=255) cGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAAccc < 1:338818/130‑1 (MQ=255) cGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCa < 8:309767/135‑1 (MQ=255) gctgctCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAAt < 3:339309/135‑1 (MQ=255) gctgctCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAAt > 6:154200/1‑135 (MQ=255) ctgctCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATg < 7:48282/135‑1 (MQ=255) gggCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGCGCTGCTGGCATCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTccgc > 2:72621/1‑135 (MQ=255) ggCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTgcg < 8:229020/133‑1 (MQ=255) cGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTCCCCAGAAAACCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTaca < 5:306013/135‑1 (MQ=255) cACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCtt > 2:374764/1‑135 (MQ=255) cACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCtt > 1:374861/1‑135 (MQ=255) cACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCtt > 6:62817/1‑135 (MQ=255) tCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTgcggcg < 5:87669/135‑1 (MQ=255) tCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTgcggcg > 8:98905/1‑135 (MQ=255) gTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTGCggcggc < 2:219284/135‑1 (MQ=255) aGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTGCGGCGGCCAAGTTGCTgg > 8:127938/1‑135 (MQ=255) | TGGAGTGGTTACGTCAGTGGCGCCTACGACTTTCTGCCGGTGATCGGTGCCATGGGCGCGAAACAGAACATTTACTACGCCACCGGCTGCTCGGGCCACGGGCTGGGCACGCAATCGTTGATCGGCAAGCTGTTTGCCCAGCAAATCAACGGCCAGGCGCCTGAGCTGCTGGCAGCACTGCAACACAAAACGCCGTCCTTGCTGCCTGAACCCTTGCAATGGTGCGCCGTACACGCTGCCTTTGCGGCGGCCAAGTTGCTGG > ACB122/3194786‑3195047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |