Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,312,431 T→G 28.1% T258P (ACG→CCG)  cynR_3 ← HTH‑type transcriptional regulator CynR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,312,4310TG28.1% 33.7 / 3.3 36T258P (ACG→CCG) cynR_3HTH‑type transcriptional regulator CynR
Reads supporting (aligned to +/- strand):  ref base T (17/7);  new base G (5/6);  total (23/13)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACAACTGCTGCGGTGCCAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTT  >  ACB122/3312297‑3312562
                                                                                                                                       |                                                                                                                                   
cGACAACTGCTGCGGTGCCAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCACCCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGt                                                                                                                                     >  2:81888/1‑135 (MQ=255)
     aCTGCTGCGGTGCCAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGa                                                                                                                                >  3:280258/1‑135 (MQ=255)
            cGGTGCCAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCGTGATGGTCACCGTCTCGATGCGCt                                                                                                                          >  4:274523/1‑135 (MQ=255)
                 ccAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCt                                                                                                                    >  4:149309/1‑135 (MQ=255)
                 ccAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCGTGCCCt                                                                                                                    >  3:285779/1‑135 (MQ=255)
                                  aaGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCGCGATGCGCTTGCCCGCCACCGACCACGGCCgg                                                                                                   >  8:156212/1‑135 (MQ=255)
                                  aaGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCGAGATGAGCTTTCCCTCCAGCCCCCAccgccgc                                                                                                   >  6:302334/1‑134 (MQ=255)
                                        ttGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCCCGCTCATCGGCGTGACCGGGTCGCTGATGGTCCCCGTCTCGCTGCGCTTGCCCGCCCGCGACCCCGGCCGGTGcccc                                                                                             >  4:218261/1‑135 (MQ=255)
                                        ttGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGGAcc                                                                                             >  3:263125/1‑135 (MQ=255)
                                        ttGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGACTAGATTCGCATGCCCTCCAGCGACCACCGCCCGTGCAcc                                                                                             >  3:307034/1‑135 (MQ=255)
                                           cccACCGCCTGCATCGCCGGGCTGAAGGCCCGTCCCCGGGCCCAGGCCACCCCCACGCTCCTCGGCGTGACCGGGGCGCTGAGGGGCACCGTCTCGATGCGCTTGCCCCCCCGCGCCCACGGCCGGTGCACCAgg                                                                                          <  6:11070/133‑1 (MQ=255)
                                                cGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCGCGATGCGCTTGCCCTCCAGCGCCCACGGCCGGGGCACCCGGTCCgc                                                                                     >  6:257124/1‑134 (MQ=255)
                                                   cTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCCCCGGCTCGCTGCGCTTGCCCGCCCGCGCCCACCGCCCGTGCCCCAGGGCCGACAg                                                                                  >  2:365691/1‑135 (MQ=255)
                                                             gggCTGAATGCCCGTTCCCGGTGCCAGGCAAACCACACGCTAATCGGCGTGACCGGGTCGCTGATGGTCCCCGTCTCGATGCGCTTGCCCTCCAGCGCCCACGGCCGGTGCCCCAGGTCCGCCAGGATCGCCCCt                                                                        >  8:303562/1‑135 (MQ=255)
                                                             gggCTGAATGCCCGTGCCCGGGCCCAGCCCAACCCCACGCGCAGCGGCGTGACGGGGTCGCTGATGGTCACCGGCTCGAGGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACt                                                                        <  3:28937/135‑1 (MQ=255)
                                                               gCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTGCCCGGCGCGATGCGCTTGCCCTCCAGCGCCCCCGGCCGGGGCACCAGGTCCGACCGGATCGCCACTcc                                                                      >  5:20078/1‑135 (MQ=255)
                                                                      gCCCGTGCCCGGGGCCAGGCCAACCCCACGCTCATCGGCGTGACGGGGTCGCTGATGGTCACCGGCTCGATGCGCTGGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGcc                                                                <  7:279020/134‑1 (MQ=255)
                                                                        ccGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGCTGCGCGTGCCCGCCCCCGCCCCCCGCCGGTGCACCAGGTCCGACCCGATCGCCCCGCCGCTGCCagt                                                             >  7:318093/1‑133 (MQ=255)
                                                                          gtccccGGTGCCCGGCCAACCCCCCGCTCAGCGGCGTGACCGGGTCGCTGATGGGCACCGGCTCGAGGCGCTGGCCCCCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTgg                                                           <  3:90267/132‑1 (MQ=255)
                                                                             cccGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGCTGCGCTTGCCCGCCAGCGACCACGGCCGGTGCACCCGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCgc                                                        >  3:306786/1‑134 (MQ=255)
                                                                               cGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGAcc                                                      <  4:280258/135‑1 (MQ=255)
                                                                                ggTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGGCTCGCTGCGCGTGCCCGCCCCCGCCCCCGGCCGGGGCCCCCGGTCCGACAGGATCGCGCCTCCGCAGCCCGTGGCGcccc                                                     >  5:15649/1‑134 (MQ=255)
                                                                                     cAGCCCAACCCCACGCTCATCGGCGTGACCGGGTCGCGGAGGGTCCCCGTCTCGATGCGCTGGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCAtgctg                                                <  4:285779/135‑1 (MQ=255)
                                                                                               ccACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTTCCCTCCAGCGCCCACGGCCGGAGCCCCAGGTCCGACAGGATAGCCACTCCGCTGCCATTTGCGCCCAAGCTTCGCACGGCCt                                      >  7:168141/1‑135 (MQ=255)
                                                                                               ccACGCTCATCGGCGTGACCGGGTCGCGGATTGTCACCGGCTCGCTGCGCTTGCCCTCCAGCGCCCACCGCCGGGGCCCCCCGGCCGCCAGGATCGCCCCGCCCCTGCCCTTGGCGCCCAAGCTGCGCCCGGCCt                                      >  2:157053/1‑135 (MQ=255)
                                                                                                     tCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCAc                                  >  8:115093/1‑133 (MQ=255)
                                                                                                     tCATCGGCGTGACCGGGTCGCTGATGGTCACCCTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCCCACGGTCCGACAGCAACGCCACTCCGCTGCCCTTGGCGACCATGCTGCGCACGGCcaccac                                  >  7:89861/1‑133 (MQ=255)
                                                                                                          ggCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGCCAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCCCGGCCTCCACCGCGCTg                           >  1:22936/1‑135 (MQ=255)
                                                                                                          ggCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTACGACAGGATCGCCACTACGCTGCCATTGCGACCATGCTTCGCACGGCCTCCCCCGAGCggg                          >  5:131599/1‑135 (MQ=255)
                                                                                                          ggCGTGACCGGGTCGCTGATGGTCACCGGCTCGATGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACGCCCCTGCCATTGGCGACCATGCTGCGCACCGCCGCCACCGAGCTg                           >  6:348448/1‑135 (MQ=255)
                                                                                                            ggTGACGGGGGCGGTGTTGGGCACCGTCTCGATGCGGTTGCCCCCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  8:168141/134‑1 (MQ=255)
                                                                                                            cgggACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  8:127292/132‑1 (MQ=255)
                                                                                                            cGTGCCCGGGGCGCTGATGGGCACGGGCTCGAGGCGCTGGCCCTCCCGCGCCCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  3:218261/135‑1 (MQ=255)
                                                                                                            cGTGACCGGGTCGCTGATGGCCACGGGCTCGATGCGCTGGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  3:136701/135‑1 (MQ=255)
                                                                                                            cGTGACCGGGGCGCTGATGGCCCCGGGCTCGATGCGCTTGCCCGCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  3:11581/135‑1 (MQ=255)
                                                                                                            cGTCACCGGGTCGCGGAGGGGCCCCGTCTCGAGGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  7:28986/135‑1 (MQ=255)
                                                                                                                                    cggTCTGGAGGCGCTTCCCCTCCGGCCTCCACGGCCGGTGCACGAGGTGCGACAGTATCGCCACTCCGCTGCCATTGGCGACGATGCTGCGCACGGCCTCCACCGAGCTGGTTCGCACCCTTACTCTTGGCCGtt  <  8:128146/133‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
CGACAACTGCTGCGGTGCCAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTT  >  ACB122/3312297‑3312562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: