Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,312,455 A→C 23.6% S250A (TCG→GCG)  cynR_3 ← HTH‑type transcriptional regulator CynR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,312,4550AC23.6% 36.8 / 2.5 41S250A (TCG→GCG) cynR_3HTH‑type transcriptional regulator CynR
Reads supporting (aligned to +/- strand):  ref base A (13/16);  new base C (9/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 7.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

AAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCAT  >  ACB122/3312331‑3312587
                                                                                                                            |                                                                                                                                    
aaGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCGCGATGCGCTTGCCCGCCACCGACCACGGCCgg                                                                                                                            >  8:156212/1‑135 (MQ=255)
aaGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCGAGATGAGCTTTCCCTCCAGCCCCCAccgccgc                                                                                                                            >  6:302334/1‑134 (MQ=255)
      ttGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCCCGCTCATCGGCGTGACCGGGTCGCTGATGGTCCCCGTCTCGCTGCGCTTGCCCGCCCGCGACCCCGGCCGGTGcccc                                                                                                                      >  4:218261/1‑135 (MQ=255)
      ttGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGGAcc                                                                                                                      >  3:263125/1‑135 (MQ=255)
      ttGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGACTAGATTCGCATGCCCTCCAGCGACCACCGCCCGTGCAcc                                                                                                                      >  3:307034/1‑135 (MQ=255)
         cccACCGCCTGCATCGCCGGGCTGAAGGCCCGTCCCCGGGCCCAGGCCACCCCCACGCTCCTCGGCGTGACCGGGGCGCTGAGGGGCACCGTCTCGATGCGCTTGCCCCCCCGCGCCCACGGCCGGTGCACCAgg                                                                                                                   <  6:11070/133‑1 (MQ=255)
              cGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCGCGATGCGCTTGCCCTCCAGCGCCCACGGCCGGGGCACCCGGTCCgc                                                                                                              >  6:257124/1‑134 (MQ=255)
                 cTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCCCCGGCTCGCTGCGCTTGCCCGCCCGCGCCCACCGCCCGTGCCCCAGGGCCGACAg                                                                                                           >  2:365691/1‑135 (MQ=255)
                           gggCTGAATGCCCGTTCCCGGTGCCAGGCAAACCACACGCTAATCGGCGTGACCGGGTCGCTGATGGTCCCCGTCTCGATGCGCTTGCCCTCCAGCGCCCACGGCCGGTGCCCCAGGTCCGCCAGGATCGCCCCt                                                                                                 >  8:303562/1‑135 (MQ=255)
                           gggCTGAATGCCCGTGCCCGGGCCCAGCCCAACCCCACGCGCAGCGGCGTGACGGGGTCGCTGATGGTCACCGGCTCGAGGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACt                                                                                                 <  3:28937/135‑1 (MQ=255)
                             gCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTGCCCGGCGCGATGCGCTTGCCCTCCAGCGCCCCCGGCCGGGGCACCAGGTCCGACCGGATCGCCACTcc                                                                                               >  5:20078/1‑135 (MQ=255)
                                    gCCCGTGCCCGGGGCCAGGCCAACCCCACGCTCATCGGCGTGACGGGGTCGCTGATGGTCACCGGCTCGATGCGCTGGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGcc                                                                                         <  7:279020/134‑1 (MQ=255)
                                      ccGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGCTGCGCGTGCCCGCCCCCGCCCCCCGCCGGTGCACCAGGTCCGACCCGATCGCCCCGCCGCTGCCagt                                                                                      >  7:318093/1‑133 (MQ=255)
                                        gtccccGGTGCCCGGCCAACCCCCCGCTCAGCGGCGTGACCGGGTCGCTGATGGGCACCGGCTCGAGGCGCTGGCCCCCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTgg                                                                                    <  3:90267/132‑1 (MQ=255)
                                           cccGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGCTGCGCTTGCCCGCCAGCGACCACGGCCGGTGCACCCGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCgc                                                                                 >  3:306786/1‑134 (MQ=255)
                                             cGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGAcc                                                                               <  4:280258/135‑1 (MQ=255)
                                              ggTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGGCTCGCTGCGCGTGCCCGCCCCCGCCCCCGGCCGGGGCCCCCGGTCCGACAGGATCGCGCCTCCGCAGCCCGTGGCGcccc                                                                              >  5:15649/1‑134 (MQ=255)
                                                   cAGCCCAACCCCACGCTCATCGGCGTGACCGGGTCGCGGAGGGTCCCCGTCTCGATGCGCTGGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCAtgctg                                                                         <  4:285779/135‑1 (MQ=255)
                                                             ccACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTTCCCTCCAGCGCCCACGGCCGGAGCCCCAGGTCCGACAGGATAGCCACTCCGCTGCCATTTGCGCCCAAGCTTCGCACGGCCt                                                               >  7:168141/1‑135 (MQ=255)
                                                             ccACGCTCATCGGCGTGACCGGGTCGCGGATTGTCACCGGCTCGCTGCGCTTGCCCTCCAGCGCCCACCGCCGGGGCCCCCCGGCCGCCAGGATCGCCCCGCCCCTGCCCTTGGCGCCCAAGCTGCGCCCGGCCt                                                               >  2:157053/1‑135 (MQ=255)
                                                                   tCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCAc                                                           >  8:115093/1‑133 (MQ=255)
                                                                   tCATCGGCGTGACCGGGTCGCTGATGGTCACCCTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCCCACGGTCCGACAGCAACGCCACTCCGCTGCCCTTGGCGACCATGCTGCGCACGGCcaccac                                                           >  7:89861/1‑133 (MQ=255)
                                                                        ggCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGCCAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCCCGGCCTCCACCGCGCTg                                                    >  1:22936/1‑135 (MQ=255)
                                                                        ggCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTACGACAGGATCGCCACTACGCTGCCATT‑GCGACCATGCTTCGCACGGCCTCCCCCGAGCggg                                                   >  5:131599/1‑135 (MQ=255)
                                                                        ggCGTGACCGGGTCGCTGATGGTCACCGGCTCGATGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACGCCCCTGCCATTGGCGACCATGCTGCGCACCGCCGCCACCGAGCTg                                                    >  6:348448/1‑135 (MQ=255)
                                                                          ggTGACGGGGGCGGTGTTGGGCACCGTCTCGATGCGGTTGCCCCCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  8:168141/134‑1 (MQ=255)
                                                                          cgggACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  8:127292/132‑1 (MQ=255)
                                                                          cGTGCCCGGGGCGCTGATGGGCACGGGCTCGAGGCGCTGGCCCTCCCGCGCCCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  3:218261/135‑1 (MQ=255)
                                                                          cGTGACCGGGTCGCTGATGGCCACGGGCTCGATGCGCTGGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  3:136701/135‑1 (MQ=255)
                                                                          cGTGACCGGGGCGCTGATGGCCCCGGGCTCGATGCGCTTGCCCGCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  3:11581/135‑1 (MQ=255)
                                                                          cGTCACCGGGTCGCGGAGGGGCCCCGTCTCGAGGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                  <  7:28986/135‑1 (MQ=255)
                                                                                                 cggTCTGGAGGCGCTTCCCCTCCGGCCTCCACGGCCGGTGCACGAGGTGCGACAGTATCGCCACTCCGCTGCCATTGGCGACGATGCTGCGCACGGCCTCCACCGAGCTGGTTCGCACCCTTACTCTTGGCCGtt                           <  8:128146/133‑1 (MQ=255)
                                                                                                     ctcGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGccc                       <  4:263125/135‑1 (MQ=255)
                                                                                                     ctcGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGccc                       <  1:84672/135‑1 (MQ=255)
                                                                                                     ctcGAGGCGCTTCCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGccc                       <  1:22193/135‑1 (MQ=255)
                                                                                                          tGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCt                  <  1:81888/135‑1 (MQ=255)
                                                                                                            cgcTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGc                <  1:123521/135‑1 (MQ=255)
                                                                                                               ttGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTcc             <  8:90667/135‑1 (MQ=255)
                                                                                                                          cGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCCCCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCAt  >  3:14513/1‑135 (MQ=255)
                                                                                                                          cGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCAt  >  3:247064/1‑135 (MQ=255)
                                                                                                                          cGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCAt  >  2:370024/1‑135 (MQ=255)
                                                                                                                            |                                                                                                                                    
AAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCAT  >  ACB122/3312331‑3312587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑