Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,412,899 G→A 5.8% P85S (CCT→TCT)  lexA_2 ← LexA repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,412,8990GA 5.8% 213.6 / 2.1 69P85S (CCT→TCT) lexA_2LexA repressor
Reads supporting (aligned to +/- strand):  ref base G (26/39);  new base A (2/2);  total (28/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GTCACCAAGTACCTTTAGGGCATAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTACCCCATACTCCAGCCATTCGCGGCGTACGTTC  >  ACB122/3412765‑3413029
                                                                                                                                      |                                                                                                                                  
gTCACCAAGTACCTTTAGGGCATAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAgg                                                                                                                                    <  8:70330/135‑1 (MQ=255)
  cACCAAGTACCTTTAGGGCATAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCa                                                                                                                                  >  3:262674/1‑135 (MQ=255)
     cAAGTACCTTTAGGGCATAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCt                                                                                                                               <  3:290928/135‑1 (MQ=255)
      aaGTACCTTTAGGGCATAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCtt                                                                                                                              >  5:23092/1‑135 (MQ=255)
          aCCTTTAGGGCATAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCg                                                                                                                          <  8:301367/135‑1 (MQ=255)
            cTTTAGGGCATAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGaa                                                                                                                        <  6:212687/135‑1 (MQ=255)
              ttAGGGCATAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAAc                                                                                                                      >  4:187378/1‑135 (MQ=255)
                aGGGCATAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACg                                                                                                                     <  3:187378/134‑1 (MQ=255)
                 gggCATAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCt                                                                                                                   >  1:249165/1‑135 (MQ=255)
                      tAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGt                                                                                                              >  3:13169/1‑135 (MQ=255)
                         ccGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACg                                                                                                                     <  4:285134/125‑1 (MQ=255)
                         ccGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACg                                                                                                                     <  6:346313/125‑1 (MQ=255)
                         ccGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACg                                                                                                                     >  5:346313/1‑125 (MQ=255)
                         ccGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACg                                                                                                                     >  3:285134/1‑125 (MQ=255)
                         ccGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTAtt                                                                                                           >  1:84458/1‑135 (MQ=255)
                          cGCTGTCGCTGGTGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTc                                                                                                          <  7:200990/135‑1 (MQ=255)
                          cGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTAtt                                                                                                           >  8:225672/1‑134 (MQ=255)
                          cGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTc                                                                                                          <  2:332578/135‑1 (MQ=255)
                               tCGCTGATGGGGACTGGGCATTACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTACACTc                                                                                                     >  1:189948/1‑135 (MQ=255)
                                       ggggACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGa                                                                                             >  2:252249/1‑135 (MQ=255)
                                           aCTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGa                                                                                         <  6:23092/135‑1 (MQ=255)
                                           aCTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGa                                                                                         <  6:370848/135‑1 (MQ=255)
                                           aCTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGa                                                                                         <  8:44612/135‑1 (MQ=255)
                                                gCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGc                                                                                     >  5:339467/1‑134 (MQ=255)
                                                 cATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCg                                                                                   <  2:115451/135‑1 (MQ=255)
                                                  aTGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGCACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGa                                                                                  >  6:318184/1‑135 (MQ=255)
                                                       agccagcTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCa                                                                                                                >  5:358259/1‑100 (MQ=255)
                                                       agccagcTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCa                                                                                                                <  6:358259/100‑1 (MQ=255)
                                                         ccagcTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGAAg                                                                           <  3:203647/135‑1 (MQ=255)
                                                           agcTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTAATCCACTCACATTGGATTGATCGCCGACCTGGAGtt                                                                         <  3:49084/135‑1 (MQ=255)
                                                            gcTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTGTTCCACTCACATTGGATTGATCGCCGACCTGGAGTTg                                                                        >  5:343923/1‑135 (MQ=255)
                                                            gcTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTg                                                                        >  2:88177/1‑135 (MQ=255)
                                                              tATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGt                                                                      <  7:218151/135‑1 (MQ=255)
                                                                     cTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAAcc                                                                 <  2:241616/133‑1 (MQ=255)
                                                                     cTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAAcc                                                                 <  2:75484/133‑1 (MQ=255)
                                                                         tAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCTCTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTg                                                           <  6:339467/135‑1 (MQ=255)
                                                                                 aaaaTTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTc                                                   >  6:169061/1‑135 (MQ=255)
                                                                                 aaaaTTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCGTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGCCCTGGAGTTGGTGAACCTTACTGTTAGGCTc                                                   >  7:41000/1‑135 (MQ=255)
                                                                                 aaaaTTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGACACCt                                                                                                                               <  4:259144/59‑1 (MQ=255)
                                                                                 aaaaTTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGACACCt                                                                                                                               <  4:254355/59‑1 (MQ=255)
                                                                                 aaaaTTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGACACCt                                                                                                                               >  3:254355/1‑59 (MQ=255)
                                                                                 aaaaTTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGACACCt                                                                                                                               >  3:259144/1‑59 (MQ=255)
                                                                                  aaaTTTGAGATTGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTTGTGAACCTTACTGTTAGGCt                                                    <  4:62576/133‑1 (MQ=255)
                                                                                  aaaTTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCt                                                    <  1:88177/133‑1 (MQ=255)
                                                                                         agaTGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCt                                            <  2:249165/134‑1 (MQ=255)
                                                                                          gaTGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCttt                                          <  2:84458/135‑1 (MQ=255)
                                                                                            tgtcctCACACCATCCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAg                                        <  3:195349/130‑1 (MQ=255)
                                                                                            tGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAg                                        <  5:148459/135‑1 (MQ=255)
                                                                                            tGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAg                                        <  3:191247/135‑1 (MQ=255)
                                                                                             ggCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAgg                                       <  1:224295/135‑1 (MQ=255)
                                                                                                   acacCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTAc                                 <  1:174990/135‑1 (MQ=255)
                                                                                                   acacCATGCCCCCGCTAGAACCCAGGAGATTAGAGGCCCATTCCCGAAACGCTCCAGTATTCCCCTCACATTGGATTGATCGCAGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTAc                                 >  5:167458/1‑135 (MQ=255)
                                                                                                    cacCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTAc                                 <  4:13169/134‑1 (MQ=255)
                                                                                                    cacCATGCCCCCGCTTGAACCCAGACGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTAcc                                <  6:215790/135‑1 (MQ=255)
                                                                                                    cacCATGCCCCCGCTTCAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTAc                                 <  3:189902/134‑1 (MQ=255)
                                                                                                            ccccGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCg                                                                                   >  2:168224/1‑76 (MQ=255)
                                                                                                            ccccGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCt                                                                               <  7:120609/80‑1 (MQ=255)
                                                                                                            ccccGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCt                                                                               >  8:120609/1‑80 (MQ=255)
                                                                                                            ccccGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTACCCCATACt                         >  8:328029/1‑134 (MQ=255)
                                                                                                            ccccGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTACCCCATACt                         >  5:265392/1‑134 (MQ=255)
                                                                                                            ccccGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGCTGAACCTTACTGTAAGGCTCCAGGGATTTAGTCAGTACCCCATACt                         >  7:225607/1‑134 (MQ=255)
                                                                                                            ccccGCTTGAACCAAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCg                                                                                   <  1:168224/76‑1 (MQ=255)
                                                                                                                     aaCCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTACCCCATACTCCAGCCAt                 <  7:225672/133‑1 (MQ=255)
                                                                                                                       cccAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTACCCCATACTCCAGCCATTcgc             <  6:273159/135‑1 (MQ=255)
                                                                                                                             agaTTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTACCCCATACTCCAGCCATTCGCGGCGTa       >  6:5614/1‑135 (MQ=255)
                                                                                                                               aTTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGtt                                                                         >  8:79513/1‑67 (MQ=255)
                                                                                                                               aTTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGtt                                                                         <  7:79513/67‑1 (MQ=255)
                                                                                                                                ttAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACTTTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTACCCCATACTCCAGCCATTCGCGGCGTACGt    <  1:135782/135‑1 (MQ=255)
                                                                                                                                ctAGCGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATGGCTCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCGTGGGCTTTAGGGAGTACCCCATACTCCAGCCATTCGCGGCGTACGt    <  7:100364/134‑1 (MQ=255)
                                                                                                                                   gagGCACCTTCCCGAAACGCTACAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTACCCCATACTCCAGCCATTCGCGGCGTACGTTc  <  4:65451/134‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GTCACCAAGTACCTTTAGGGCATAACCGCTGTCGCTGATGGGGACTGGGCATGACAGCCAGCTATCTGCCTGGTAGGGCTCAAAATTTGAGATGGCTTCACACCATGCCCCCGCTTGAACCCAGGAGATTAGAGGCACCTTCCCGAAACGCTCCAGTATTCCACTCACATTGGATTGATCGCCGACCTGGAGTTGGTGAACCTTACTGTTAGGCTCCTGGGCTTTAGGGAGTACCCCATACTCCAGCCATTCGCGGCGTACGTTC  >  ACB122/3412765‑3413029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑