| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,440,343 | A→C | 100% | S18R (AGC→CGC) | CJ019_03266 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,440,343 | 0 | A | C | 94.9% | 22.5 / ‑6.1 | 23 | S18R (AGC→CGC) | CJ019_03266 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (14/6); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA > ACB122/3440211‑3440473 | gATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGccgcccgc > 5:215868/1‑135 (MQ=255) gTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTGCCGgcgc > 5:112671/1‑135 (MQ=255) aaTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCggg > 6:187208/1‑135 (MQ=255) aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGc > 7:339505/1‑135 (MQ=255) tAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCCGCCggg > 2:109447/1‑135 (MQ=255) gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCCGCGCCCGGGCACCCTGGgt > 4:95799/1‑135 (MQ=255) catcGGTCGGGACGCCCGCACGGCCCACCACCCGCGCGCGCGCGCGGGGCTGCAGTGCGGGGTGGGGCCCCCCGCCGGGGCGCCGGGGCGCTGCGGCGGCCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGgtg < 8:39121/132‑1 (MQ=255) cAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCCGGgtg > 1:179740/1‑135 (MQ=255) ggaggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCGGGGGGTggccggcc > 4:152775/1‑135 (MQ=255) ggaggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCTGGGGGTggccggcc > 8:334380/1‑135 (MQ=255) cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCCGCCGGGGGGTGGGCGGCCACCGCCta > 5:356765/1‑134 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCAGGCGGCGCCCGCGCCGCCGGGGTGTGGCAGGCCAACGGCgata > 3:95086/1‑135 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACACGCCTGGTCGCCGTGGCGCTGCTGCTGGGCCCCCGCGCAGGCGGCGCCCCGCCAGCCGCGGGGTGTGAGGCCCACGCCCAAAAGGCGCACCGCGCGGAtacg > 1:154420/1‑132 (MQ=255) gcgcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCACGCAGGCGGGGCCCGGGGCGCCTGGGTGGGGCACGCCAACGCCTATAAGGCGCAACTCGCTGATCAGACCGCAGACGTGGGCAAGc > 8:169618/1‑135 (MQ=255) cgggggCCCCCCGCCGGGGCGCCGTGGCGCGCGCCCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGt < 5:237230/132‑1 (MQ=255) ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCTGGGGGTGGCGGGCCACCGCCGAAAAGGCGCCGCGCGCTGCTAAGGGCGAGGAGTTCGGCCAGCACGTGGc > 4:314333/1‑135 (MQ=255) cGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGCCCCCGCCGCTAAGGCGCAGCGCGCGGCGCAGGCCGCGGCCGTCGGCCAGCCGTGGGCggggccg > 3:187385/1‑130 (MQ=255) gggggTGGCGCGGCGGGCGCCCGCCCGCGCGGGCGCCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 5:7684/131‑1 (MQ=255) ggCCGGGGCGCTGGGGCGGGGCGGGTGCGCGTGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 6:318182/134‑1 (MQ=255) cgggggggCGCTGCGGCGGCCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 7:111255/129‑1 (MQ=255) cgcgGTGGGGGTGCTGGGGGCCGCCCGCGCTGGGGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 7:203824/135‑1 (MQ=255) ctgcggcggGCCGGCCGCGGCGCCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTg < 3:109450/135‑1 (MQ=255) cgccgccGGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGga < 6:215868/134‑1 (MQ=255) cgccCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa < 3:152775/135‑1 (MQ=255) | GATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA > ACB122/3440211‑3440473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |