Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,485,289	A→C	17.0%	F63V (TTC→GTC)	CJ019_03301 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,485,289	0	A	C	17.0%	81.6 / 2.1	64	F63V (TTC→GTC)	CJ019_03301	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/15);  new base C (4/9);  total (40/24)
Fisher's exact test for biased strand distribution p-value = 1.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGAATTCGAATCGGTGCG  >  ACB122/3485157‑3485417                                                                                                                                     |                                                                                                                                 aCGGGCGTGGCAGCACGCCCGACGGCCCGCCCAGGCCCATGAAGGTGACCTGCACGTCCGACAGCGGCAAGCCGGCCGGCGCCACCTTGCCGTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGCACg                                                                                                                                >  8:336661/1‑135 (MQ=255)          gcagcaCGCCCGCCGGCCCGCCCAGGCCCATGAAGGTGACCTGCACTTCCGACCGCGGCAAGCCGGCCGGCGCCACCTTGCCGTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGCACGCCAGCGACa                                                                                                                       >  7:341024/1‑135 (MQ=255)           ccgcaCGCCCGCCGGCCCGGCCAGGCCCATGAAGGTTCCCTGCACTTCCGCCCGCGGCAAGCCGGCCGGCGACACCTTTCCGTCGCGCTCGCAGGGCAGGTCGCTGACCTCGGGGGGCGGGGAcgccagcgccag                                                                                                                      >  1:136700/3‑135 (MQ=255)           cagcaCGCCCGACGGCCCGCCCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACa                                                                                                                       >  6:366159/1‑134 (MQ=255)           cagcaCGCCCGACGGCCCGACCAGGCCCCTGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCGTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGCACGCCAGCGACAg                                                                                                                      >  4:147701/1‑135 (MQ=255)           cagcaCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGCCAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGCCCGCGCTGGCCGGGAACGCCCGCGACAg                                                                                                                      >  8:354988/1‑135 (MQ=255)           cagcaCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGCACGCCCGCGAccg                                                                                                                      >  8:201641/1‑133 (MQ=255)           cagcaCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAg                                                                                                                      >  2:43854/1‑135 (MQ=255)           cagcaCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAg                                                                                                                      >  2:52957/1‑135 (MQ=255)           cagcaCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAg                                                                                                                      >  3:117881/1‑135 (MQ=255)           cagcaCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCGGGCCGGGAACGCCCGCGACAg                                                                                                                      >  5:297154/1‑135 (MQ=255)                  cccGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGCCAGCTGGGTa                                                                                                               >  7:151461/1‑135 (MQ=255)                  cccGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTa                                                                                                               >  4:220272/1‑135 (MQ=255)                  cccGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTa                                                                                                               >  7:146796/1‑135 (MQ=255)                     cccGGCCCGACCAGGCCCTTGAAGGGGCCCTGCACTTCCGACCGCGGCACGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCGGGCCGGGAACGCCAGCGACAGCTGGGTACGa                                                                                                            <  6:182125/133‑1 (MQ=255)                      aCGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGCCACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGCCCTCGCTGGCCGGGAACGCCAGCGCCAGCGGGGTTCGa                                                                                                            >  2:168317/1‑134 (MQ=255)                           ccGCCCAGGCCCTGCACGGGGACCTGCAGTGCCTACCGCGGCAAGCCGGCCGGCGACACCTTGCCTGCGCCCTCGAAGTGCTGGTCGCTGACCTCGCGGGCCGGGAACCCCCGCGACAGCTGGGTACGAAAACGc                                                                                                      <  7:283201/135‑1 (MQ=255)                             gACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAg                                                                                                    >  8:61050/1‑135 (MQ=255)                                cAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCgg                                                                                                 >  7:222016/1‑135 (MQ=255)                                    cccATGAAGGGCCCCGCCACTTCCGCCCGCGGCACGCCGGCCGGCGACACCTGGCCTGCGCGCGCGAAGGGCAGGGCGCGGACCTCGCGGGCCGGCAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCt                                                                                             <  2:259422/135‑1 (MQ=255)                                       aTGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCg                                                                                          >  4:28208/1‑135 (MQ=255)                                           aGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCg                                                                                      >  7:117929/1‑135 (MQ=255)                                           aGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCg                                                                                      >  7:99862/1‑135 (MQ=255)                                           aGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTCCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCg                                                                                      >  7:222469/1‑135 (MQ=255)                                            ggTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGACCGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCgg                                                                                     >  6:171811/1‑135 (MQ=255)                                             gTGCCCTGCACTCCCCACCGCGGCACGCCGGCCGGCGACACCTTGCCTGCGCGCGCGAAGGGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGc                                                                                    <  7:45393/135‑1 (MQ=255)                                                aCCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGCACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCgg                                                                                 >  6:298754/1‑135 (MQ=255)                                                aCCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGACCGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCgg                                                                                 >  6:154385/1‑135 (MQ=255)                                                aCCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCgg                                                                                 >  7:295589/1‑135 (MQ=255)                                                aCCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCgg                                                                                 >  8:121724/1‑135 (MQ=255)                                                   tGCACTTCCGACAGCGGCACGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGtt                                                                              <  7:322802/135‑1 (MQ=255)                                                   tGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGtt                                                                              >  5:299425/1‑135 (MQ=255)                                                   tGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGtt                                                                              >  3:93367/1‑135 (MQ=255)                                                   tGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGtt                                                                              >  8:295706/1‑135 (MQ=255)                                                   ggCACTTCCGACCGCGGCACGCCGGCCGGCGACACCTTGCCTTCGCGCTCGACGTGCTGGGCGCTGACCTCGCGGGCCGGGACCCCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGtt                                                                              <  8:151461/134‑1 (MQ=255)                                                             aCAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGACCGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCa                                                                    >  3:330562/1‑135 (MQ=255)                                                                gCGGCACGCCGGCGGGCGACACGTTGCCTGCGCGCTCGACGTGCAGGTCGCTGACCGCGCTGGCCGGGACCGCCAGGGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATgc                                                                 <  6:275364/135‑1 (MQ=255)                                                                gCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATgc                                                                 >  7:207529/1‑135 (MQ=255)                                                                gCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGGCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATgc                                                                 <  8:108236/135‑1 (MQ=255)                                                                ccGGCACGCCGGCCGGCGAAACCTGGCCTTCGCGCTCGAAGGGCAGGTCGCTGACCGCGCGGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATgc                                                                 <  6:253031/134‑1 (MQ=255)                                                                     acgccggccgGCGACCCCGGGCCTGCGCGCTCGAGGTGCTGGTCGCGGACCGCGCGGGCCGGCACCGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCg                                                            <  2:119577/133‑1 (MQ=255)                                                                      aGCGGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCgg                                                           <  7:362488/135‑1 (MQ=255)                                                                        ccggccggCTACACCTTGCCTGCGCGCGCGACGTGCTGGGCGCGGCCCTCGCGGGCGGGGACCGCAAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTg                                                         <  7:336661/135‑1 (MQ=255)                                                                        ccggccggCGACACCTTGCCTTCGCGCTCGACGTGCCGGGCGCTGACCTCGCTGGCCGGGACCGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTg                                                         <  7:223206/135‑1 (MQ=255)                                                                         cggccggcGACACCTCGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGCACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGc                                                        >  6:53498/1‑135 (MQ=255)                                                                            cgggcCACCCCTGGCGTGCGCGCTCTTTGTGCTGGAGGCGGACCGCGGGGGCCGGTACCGCCTCCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGc                                                     <  6:297154/133‑1 (MQ=255)                                                                            ccggcGACACCTTGCCTGCGCGCTCGAAGGGCAGGGCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGc                                                     <  7:195395/135‑1 (MQ=255)                                                                                    acCTTGCCTGCGCGCGCGAAGGGCAGGTCGCTGACCTCGCGGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGc                                             <  5:104582/135‑1 (MQ=255)                                                                                    acCTGGCCTCGGCGCGCTATGTGCTGGGCGGTTACCTCGCGGCCCGGCACCCCCCGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGc                                             <  4:281442/135‑1 (MQ=255)                                                                                     ccTGGCCTTCGCGCTCGACGTGCAGGTCGCGGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCa                                            <  8:105702/135‑1 (MQ=255)                                                                                          ccTCCGCGCGCGAAGGGCAGGTCGCTGACCTCGCTGGCCGGTAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCgg                                       <  3:266236/135‑1 (MQ=255)                                                                                                 gcTCGAAGTGCAGGTCGCTGCCCTCGCTGGCCGGGCACGCCAGCGCCAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGgc                                >  7:329528/1‑135 (MQ=255)                                                                                                   tCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGGCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGgcgc                              >  1:373352/1‑135 (MQ=255)                                                                                                                cGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGa                 >  1:284615/1‑135 (MQ=255)                                                                                                                   tGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGAAtt              >  6:240065/1‑135 (MQ=255)                                                                                                                      ccTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGAATTCg            <  7:121724/134‑1 (MQ=255)                                                                                                                      ccTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGAATTCGa           >  4:221199/1‑135 (MQ=255)                                                                                                                      ccTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGAATTCGa           >  1:318388/1‑135 (MQ=255)                                                                                                                          gCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGAATTCGAATCg       <  4:190589/135‑1 (MQ=255)                                                                                                                             ggCCGGGACCGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGAATTCGAATCGGTg    >  3:290166/1‑135 (MQ=255)                                                                                                                              gCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGAATTCGAATCGGTGc   >  8:229833/1‑135 (MQ=255)                                                                                                                               cggtgAAGGCCTGCGACAGCTGGGTACGAAAACGCAGCGGGGCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGAGCACCAATTGAAAGAATTCGAATCGGTGcg  <  7:27382/131‑1 (MQ=255)                                                                                                                               ccGGGACCGCCCGCGACAGCTGGGTACGAAACCGCAGCGGGTCTTCGTGCGGCCGGGTGGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGAATTCGAATCGGTGcg  <  6:113820/135‑1 (MQ=255)                                                                                                                               ccGGGACCGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGGCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGAATTCGAATCGGTGcg  <  2:10093/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                 ACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCAGCGACAGCTGGGTACGAAAACGCAGCGGGTCTTCGTGCGGCCGGGTTGCCAGGTCCATGCGCCCGGTGCGGCTGTAGTGCAGGCGGAGCAGGCGCACCAATTGAAAGAATTCGAATCGGTGCG  >  ACB122/3485157‑3485417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑