| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,491,319 | A→C | 48.4% | intergenic (‑681/+40) | CJ019_03306 ← / ← CJ019_03307 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,491,319 | 0 | A | C | 48.4% | ‑1.2 / 9.5 | 20 | intergenic (‑681/+40) | CJ019_03306/CJ019_03307 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/4); new base C (8/2); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01 | |||||||||||
CGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATG > ACB122/3491212‑3491433 | gggtgggGCCGCCCGGTTGCAGGAGGGGGTTACCCCGCCAATAGGCCGCACCGGCCGGCAACTATCCCTGGCAATCCCCCGACACACCCCCCACCGCAGCCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCg < 8:214970/134‑1 (MQ=255) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGGCGGCGGCCCCCGGACCCCGGCgccgcccc > 1:170049/1‑133 (MQ=18) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACAACGGCGCAGCCgg > 6:114566/1‑135 (MQ=255) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCCGGCCGCCGGCCCGCCCACACCCGCGCCGCCgg > 6:375645/1‑135 (MQ=21) gtgtCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGtt > 8:350478/1‑133 (MQ=255) gggcGCCAGGTTGTCGGCGGTGGTTTCCCCGCAAATCGGCCGCCCCGGCCGGCCACTAGCCCTGGCCATACCCCGCCCACCCCCCCACCGCCTGCGCGGGCCGCCGGCCCGCGAACACCGGCGCAGCCGGTTTCa < 8:145957/132‑1 (MQ=255) ggTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCAATATCCGTGGGCATTCACCGTCCCAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTc < 8:286533/134‑1 (MQ=255) gTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCCCGCACACCGGCGCCCCCCGGTTGATCCCACACAAcc > 3:331970/1‑134 (MQ=17) gAGGTGGTTTCCCCGCTATTTGGCCGTACCGGCGGGCCAATACCCCTGGGAATACCCGGTCCCCCCCGCCCCCGCATGCGCGGGCCGCCGGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGAcc < 2:205214/134‑1 (MQ=255) ggtgttttACCCGCTAATTGGCGGGACCTCCCGCCAAATACCCCTGTGCATTCCCCGCCCCACCCCCCCCCGCATCCGCGGGCAGCCTGCCCGCCAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATc < 6:281146/130‑1 (MQ=255) tttACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCGGCCCCCGAACACCGGCCCAGCCCGTTTGCTCCAACTCAACGCCCCTCGACCt > 6:63060/1‑135 (MQ=21) ttACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCTGCCCGCGAACACCCGCGCCCCCGGGGTCATCCCACTCCACGCCCCGCGCCCTg > 4:26927/1‑135 (MQ=18) tAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCGGACCGCGCACACCCGCCCCCCCGCTTGTATCCCACAAAACGACCCACCCCCCGTACCCAAAccc > 2:286262/1‑133 (MQ=12) ttCCCCGTCACCGCCGCCCCGGCATCCGGGGGCTGCGTGCCGGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGt < 4:267041/135‑1 (MQ=255) aCCGTCACAGCCGCCACCGCATTCGCGGGGCGCCTGCCCGCGAACACCGGCCCCGCCGGGTTGATACAACTAAACCCCCAGCGACCTGTACGCAAACGTCTCTGCCCACGAATTCCTTGCAGGGCCAATTTTCGt > 8:2830/1‑135 (MQ=255) aCCGTCACAGCCGCCACCGCATTCGCGGGCCGCCTGCCCGCGAACACCGGCGCAGCCGGGTTCATACAGCTCAACGACCATCGCCCTGTACGCCAACGTCTCTGCCATCGAATGCCTTGCATGGCCAATTGTCGt > 2:60430/1‑135 (MQ=255) ccGTCACAGCCGCCACCGCATTCGCGGGGAGCCTGCCCGCGCACACCGGCGCCGCCGGGTTGCTCCAACACAACGCCCATCGACCAGTACCCAAAAGGCACTGCCCTCGATTGGCGTGCATGGCCGATTGTCGtg > 8:42965/1‑134 (MQ=255) cGTCACAGCCGCCACCGCATTCGCGGGCCGCCTGCCCGCGAACAACCGCGCCGCCCGTTTAATACAACAAAAACAACATCGCCCAGTTCCGACAAGGCACAGACAACGATTTCCTTTCATGGCCAATTGTCggtt > 2:147412/1‑132 (MQ=255) cacccccgccACCGATTGCGCGGGCAGCGGGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATg < 6:288724/130‑1 (MQ=255) cgccACCGCATTCGCGGGCCGCCGGCCCGCGCACACCGGCGCAGCCGGGTTGATCCAACTCAACGCCCATCGACCGGTACGCAAACGTCTCTGGCATCGATTGGCTTGGATTGGCAATTGTGGTTGATGTTATg > 4:174434/1‑134 (MQ=255) | CGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATG > ACB122/3491212‑3491433 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |