Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,652,041 A→T 15.5% N294K (AAT→AAA)  cntA ← Metal‑staphylopine‑binding protein CntA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,652,0410AT15.5% 133.5 / 4.5 61N294K (AAT→AAA) cntAMetal‑staphylopine‑binding protein CntA
Reads supporting (aligned to +/- strand):  ref base A (32/19);  new base T (8/2);  total (40/21)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGACGTCGTATACGCCACTCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGCCACAGGATGTCGACCTTGCCTTGCAGGA  >  ACB122/3651910‑3652174
                                                                                                                                    |                                                                                                                                     
gtgACGTCGTATACGCCACTCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTg                                                                                                                                    <  8:259195/135‑1 (MQ=255)
  gACGTCGTATACGCCACTCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTggg                                                                                                                                  <  8:185820/135‑1 (MQ=255)
   aCGTCGTATACGCCACTCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTgggg                                                                                                                                 <  3:208240/135‑1 (MQ=255)
         tataCGCCACTCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGCGGGGTTTTGGGGtgagg                                                                                                                            >  5:333723/1‑131 (MQ=255)
         tataCGCCACTCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTTTTGGGGTAGAgg                                                                                                                           >  4:199567/1‑135 (MQ=255)
         tataCGCCACTCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTgggg                                                                                                                                 <  1:87342/129‑1 (MQ=255)
         tataCGCCACTCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTgggg                                                                                                                                 >  2:87342/1‑129 (MQ=255)
         tataCGCCACTCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCGTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTTTTGGGGGaggcg                                                                                                                           >  1:356155/1‑132 (MQ=255)
                   tCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCg                                                                                                                 >  3:168990/1‑135 (MQ=255)
                   tCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGGTGC‑GGGTATTGGGGTAGAGGTTCATCGCCg                                                                                                                 >  4:49416/1‑135 (MQ=255)
                      ccaccGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTTTTGGGGTAGAGGTTCATCGCCGGGc                                                                                                              >  6:155767/1‑135 (MQ=255)
                      ccaccGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTTGGGTAGCGGGTGATCGCCCGGc                                                                                                              >  5:346676/1‑135 (MQ=255)
                      ccaccGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGc                                                                                                              >  4:307384/1‑135 (MQ=255)
                             gAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAg                                                                                                       >  6:73237/1‑135 (MQ=255)
                             gAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGGCGTTGG‑GGGGTTTGGGGGTGAGGGTTATACCCGGGGCGGGCAg                                                                                                       >  5:17954/1‑135 (MQ=255)
                              aaCGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGc                                                                                                      <  6:200344/135‑1 (MQ=255)
                               aCGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGATGGTCATCGCCGGGCCTGGCAGc                                                                                                      >  6:330161/1‑134 (MQ=255)
                               aCGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCAACGCCGGGCCTGGCCGc                                                                                                      >  5:117436/1‑134 (MQ=255)
                               aCGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGACCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGc                                                                                                      >  6:149113/1‑134 (MQ=255)
                                   aGTGTTGTAGACGGTGTGCGCGTAGCGGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACg                                                                                                 <  3:138153/135‑1 (MQ=255)
                                        tgtAGACGGTGGGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGGCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGctct                                                                                            <  6:187585/135‑1 (MQ=255)
                                              cggggggcgcGTAGGTGGGCCGGTCGAGGGCCGGCTGCCGCGCCAGGCGCAGGTGGCGGTCAGCGAGCAGGCGGTCGTGGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGCCAGCGAACGGCTCTGGATgg                                                                                      <  6:235000/129‑1 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCCCTTGGCGGTCACCGAGCAGGCGGTCGTTGG‑GGGTATTGGGGGAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCGGGATGGTCgcgc                                                                                >  6:81221/1‑135 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGG‑GGGGTTTGGGGTAGAGGTTCATCGCCGGGCCTGGCCGCGCACCGCGCGGGATGGtggggc                                                                                >  1:376201/1‑130 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTTGCAGCGAGCGGCTCTTGATTGTCgcgc                                                                                >  2:229121/1‑135 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCCGCGAACGGCTCTGGATGGTCgcgc                                                                                >  7:280049/1‑135 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGctct                                                                                            <  2:199702/123‑1 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGctct                                                                                            >  1:199702/1‑123 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATgg                                                                                      >  1:36427/1‑129 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATgg                                                                                      <  2:36427/129‑1 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCgcgc                                                                                >  8:289160/1‑135 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCgcgc                                                                                >  5:161359/1‑135 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCgcgc                                                                                >  7:302869/1‑135 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCGCTGGATGGTCgcgc                                                                                >  4:51034/1‑135 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGGAGAGGTTCATCGCCGGGCCTGGCCGCGAACGGGGCGGGGTGGTCgcgc                                                                                >  5:20613/1‑135 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGGAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCgcgc                                                                                >  4:341923/1‑135 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGGATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGGACGGCTCGGGGTGGTCgggc                                                                                >  3:226240/1‑135 (MQ=255)
                                                    gcgcGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTGGC‑GGGTATTGGGGGTGAGGTTCATCGCCGGGGCTGGTAGCGCACGGCTCTGGCTGGTCgcgc                                                                                >  5:288145/1‑135 (MQ=255)
                                                    gcgaGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCgcgc                                                                                >  3:114335/1‑135 (MQ=255)
                                                              ggTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTgggcg                                                                       >  7:352785/1‑131 (MQ=255)
                                                                  cGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGGAGAAGTTAAACGCCCGGCCAGGCAACGCACGGCGGAGGATGGTGGCGCCCGTGGCCAGaaaa                                                                  >  3:204062/1‑134 (MQ=255)
                                                                   ggTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGG‑GGGTTTTGGGGTAGAGGTTGATCGCCGGGCCGGGCCGCGAACGGGGCTGGATGGTCGCGCCCGTGGACTGAAACa                                                                 >  2:131771/1‑135 (MQ=255)
                                                                     tCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGc                                                               <  4:315913/135‑1 (MQ=255)
                                                                       gATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGG‑GGGTTTTGGGGTAGAGGTTCATAGCCGGGCCTGGCCGCGAACGGCTCTGGCTGGTAGCGCCCGTGGGCTGCAACAGCTg                                                             >  1:345938/1‑135 (MQ=255)
                                                                                  gCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGCACGGCTCTGGATGGTCGCGCCCTTGGACTGCAACCGCTGCAGGTCAACtt                                                  >  6:24685/1‑135 (MQ=255)
                                                                                  gCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGGCAACtg                                                  >  3:252414/1‑134 (MQ=255)
                                                                                         cAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCGTGGACTGAAACAGCTGCAGGTCAACTTCCGAGaa                                           >  3:251242/1‑135 (MQ=255)
                                                                                           ggCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACg                                         >  8:243497/1‑135 (MQ=255)
                                                                                              gcACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCTGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGt                                      <  1:131771/135‑1 (MQ=255)
                                                                                              gcACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGCACGGCTCTGGATGGTCGCGCCCGTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGt                                      >  5:267789/1‑135 (MQ=255)
                                                                                              gcACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGt                                      >  3:63368/1‑135 (MQ=255)
                                                                                                      cGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGc                              <  4:231641/135‑1 (MQ=255)
                                                                                                       ggTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCGTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGcc                             <  5:34516/135‑1 (MQ=255)
                                                                                                       ggTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGcc                             <  3:341923/135‑1 (MQ=255)
                                                                                                       ggTCACCGAGCAGGCGGGCGTTGC‑GGGTTTTGGGGTAGAGGTGCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGcc                             <  5:232341/135‑1 (MQ=255)
                                                                                                                  cggCGGTCGTTGC‑GGGTTTTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGCCACAGGATGTCg                  <  4:204062/134‑1 (MQ=255)
                                                                                                                      gggcGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGCCACAGGATGTCGAcct              <  2:376201/132‑1 (MQ=255)
                                                                                                                       gTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGCCACAGGATGTCGAcctt             >  3:160630/1‑135 (MQ=255)
                                                                                                                           ttGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGCCACAGGATGTCGAccttgcct         <  5:199354/135‑1 (MQ=255)
                                                                                                                           tggC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGCCACAGGATGTCGAccttgcct         <  3:307384/133‑1 (MQ=255)
                                                                                                                                   tATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGCCACAGGATGTCGACCTTGCCTTGCAGGa  <  4:1816/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
GTGACGTCGTATACGCCACTCACCACCGGGAACGCAGTGTTGTAGACGGTGTGCGCGTAGCTGGTGCGGTCGATGGCCTTCTGCAGCGCCAGGCGCACTTGGCGGTCACCGAGCAGGCGGTCGTTGC‑GGGTATTGGGGTAGAGGTTCATCGCCGGGCCTGGCAGCGAACGGCTCTGGATGGTCGCGCCCTTGGACTGAAACAGCTGCAGGTCAACTTCCGAGAACGGGTTGCGTGGCCACAGGATGTCGACCTTGCCTTGCAGGA  >  ACB122/3651910‑3652174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑