Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,816,362 A→C 16.6% R295R (CGT→CGG)  kdgK ← 2‑dehydro‑3‑deoxygluconokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,816,3620AC16.6% 83.4 / 3.5 50R295R (CGT→CGG) kdgK2‑dehydro‑3‑deoxygluconokinase
Reads supporting (aligned to +/- strand):  ref base A (21/19);  new base C (7/3);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTTTGCATGGTTTGTTTCTCCTGAGCTTGTTGTTTTTGTTGCAACAGGTGATTCGGGATCGGCTCATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATCACCTGCACCGACGGTGTCCACCACGCGGCT  >  ACB122/3816231‑3816496
                                                                                                                                   |                                                                                                                                      
ggctttgCATGGTTGGTTTCTCCTGAGCTGGTTGTTTTTGTTGCACCAGGTGAGTCGGGAGCGGCGCATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCCGGGCGCAGCGGCAGCCGTTCCATGGCCCCAcg                                                                                                                                      <  7:64693/134‑1 (MQ=255)
  ctttgCATGGTTTGTTTCTCCTGAGCTTGTTGTTTTTGTTGCAACAGGTGATTCGGGATCGGCTCATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCCGCGGCAGCCCTTCCATGTCCCCCCGCGa                                                                                                                                   >  5:72462/1‑135 (MQ=255)
  ctttgCATGGTTTGTTTCTCCTGAGCTTGTTGTTTTTGTTGCAACAGGTGATTCGGGATCGGCTCATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGa                                                                                                                                   >  2:65576/1‑135 (MQ=255)
         tGGTTTGTTTCTCCTGAGCTTGTTGTTTTTGTTGCAACAGGTGATTCGGGATCGGCTCATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCACCCCTTCCATGTCGCCCCGCCACTgcacg                                                                                                                            >  6:24553/1‑135 (MQ=255)
                    tcCTGAGCTTGTTGTTTTTGTTGCAACAGGTGATTCGGGATCGGCTCATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCATGCGACTGCACGGCACGGCTGcc                                                                                                                 >  8:7402/1‑135 (MQ=255)
                    tcCTGAGCTTGTTGTTTTTGTTGCAACAGGTGATTCGGGATCGGCTCATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGcc                                                                                                                 >  3:229863/1‑135 (MQ=255)
                                       gttgCAACAGGTGATTCGGGATCGGCTCATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTTGCCACGCGACTGCACGGCACGGCTGCCGCACCAGATGCCGCGCGCt                                                                                              >  7:252607/1‑135 (MQ=255)
                                        ttgCAACAGGTGATTCGGTAGCGCCTCATGCGGTTCGGCGCAGGGCGTAGGCCTCCAGCTCATGGCGCAGCGGCACCCCTACCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCt                                                                                              <  2:48730/134‑1 (MQ=255)
                                           cAACAGGTGATTCGGGATCGGCTCATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTAcc                                                                                           <  7:19519/134‑1 (MQ=255)
                                            aaCAGGGGATTCGGGATCGGCGCATGCGCTGCTGCGCAGGTCGTAGGCCGCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGGCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGc                                                                                         <  2:52220/135‑1 (MQ=255)
                                                        gggAGCGGCTCATGCGCTTCTGCGCAGGGCGTAGGCCTCCAGCTCATGGCGCTGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCg                                                                              <  4:32656/134‑1 (MQ=255)
                                                             cGGCTCATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCCCGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCCCGCGCTACCGCCGCGGCTACcggccggc                                                                        >  7:15347/1‑135 (MQ=255)
                                                              ggCTCATGCGCTTCTGCGCTGGTCGTAGGCCTCCAGCTCTTGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGcc                                                                       <  6:96842/135‑1 (MQ=255)
                                                                  cATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCCCGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTcc                                                                   >  2:242400/1‑135 (MQ=255)
                                                                  cATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTCCCGCCTCGGCGACCGGACGGCCCt                                                                     >  7:224467/1‑133 (MQ=255)
                                                                   aTGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCCCGCGACTGCACGGCACGGCTGCCGCCCCAGTTGCCGCGCGCTACCGCCTCGGCGACCGGCCGGCCCGCCa                                                                  >  2:293218/1‑135 (MQ=255)
                                                                    tGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAg                                                                 <  4:185054/135‑1 (MQ=255)
                                                                       gcTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTAcc                                                                               >  7:353193/1‑118 (MQ=255)
                                                                       gcTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTAcc                                                                               <  8:353193/118‑1 (MQ=255)
                                                                       gcTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAg                                                              >  8:374914/1‑135 (MQ=255)
                                                                       gcTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCACTAg                                                              >  2:241940/1‑135 (MQ=255)
                                                                        gttCTGCGCAGGTCGTAGGCCTCCAGCGCATGGCGCAGCGGCAGCCCTTCCATGTCGCCCCGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAgc                                                             <  6:93395/134‑1 (MQ=255)
                                                                        gttCTGCGCAGGTCGTAGGCCTCATGCTCATGGCTCAGCGGATGCCGATCCATGGCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAgc                                                             <  6:73610/134‑1 (MQ=255)
                                                                             gcgcAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAgcgcgca                                                        >  5:198435/1‑134 (MQ=255)
                                                                             gcgcAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCCCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTCCCGGACGGCCCTCCAGTAgcgcgc                                                         >  4:311261/1‑134 (MQ=255)
                                                                                 aGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGc                                                    >  1:12377/1‑135 (MQ=255)
                                                                                    gctttggCCTCCAGCACATGGCGCAGCTGAAGCCCTACCATGTCCCCCCGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGcac                                                  <  5:99917/129‑1 (MQ=255)
                                                                                      gTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCCCCAGTTGCCCCGCGCTACCGCCACGGCGACCGGTCGGCCCCCCAGTAGCGCGCGGAGCCCAcc                                               >  1:166558/1‑135 (MQ=255)
                                                                                      gTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACAcc                                               >  3:232480/1‑135 (MQ=255)
                                                                                        aGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTAcc                                                                               >  8:129912/1‑101 (MQ=255)
                                                                                        aGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTAcc                                                                               <  7:129912/101‑1 (MQ=255)
                                                                                          gCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACg                                           <  4:232480/135‑1 (MQ=255)
                                                                                           ccTCCAGCTCATGGCGCAGCGGCCGCCCTTCCATGTCGCCCCGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGCCGGCCCTCCAGTAGCGCGCTGCGCAcccccccgg                                          >  4:27559/1‑130 (MQ=255)
                                                                                                aGCTCTTGGCGCAGGGGATGCCCTTCCATGGCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAg                                     <  7:343967/135‑1 (MQ=255)
                                                                                                aGCTCATGGCGCAGCGGCAGCCCTTCCTTGGCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCaaa                                      <  5:154045/134‑1 (MQ=255)
                                                                                                aGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCCCCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAg                                     >  8:179295/1‑135 (MQ=255)
                                                                                                aGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCACTACCGCCTCGGCAACCGGACGGCCCTCCAGTAGCGCGCTGAGCCCACCGACGGCaacg                                     >  7:10870/1‑133 (MQ=255)
                                                                                                aGCTCATGGCGCAGCGGCAGCCCTTCCATGTAGCCCCGAGACTGCACGGCACGGCTGCCGCACAAGTTGCCGCTCGCTACCGCCTCGGCTACCGGCAGGCCCTCCAGTAGCGCGCTTCGCACACCGACGGCAAAg                                     >  2:53193/1‑135 (MQ=255)
                                                                                                   tCATGGCGCAGCGGCAGCCCTTCCATGTCGCCCCGCGCCTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCGCGGCGACCGGGCGGCCCGCCCGTAGCGCGCGGGGCCCCCCGCCCGCAAAGGCa                                  >  5:204121/1‑135 (MQ=255)
                                                                                                     aTGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATc                                <  4:272556/135‑1 (MQ=255)
                                                                                                          gcAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCCACGGCAAACGCATCACCTg                           >  1:131648/1‑135 (MQ=255)
                                                                                                             ggggCAGCCCTTCCTTGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATCACCTGCAc                        <  8:15347/133‑1 (MQ=255)
                                                                                                             gCGGCAGCCCTTCCATGGCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATCACCTGCAc                        <  1:65576/135‑1 (MQ=255)
                                                                                                               ggCCGCCATTCCTTGGCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATAACCTGCAc                        <  6:72462/133‑1 (MQ=255)
                                                                                                               gcccgCCCTCCCTGGTCGCCCCGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATCACCTGCACCg                      <  7:163544/131‑1 (MQ=255)
                                                                                                                gCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATCACCTGCACCGa                     <  3:261790/135‑1 (MQ=255)
                                                                                                                           aTGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGCCGGCCCTCCAGTAGCGCGCTGAGCACACCGCCGGCAAAGGCCTCCCCTGCCCCGACGGTGTccacc          >  7:335649/1‑135 (MQ=255)
                                                                                                                           aTGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGCCGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATCACCTGCACCGACGGTGTccacc          >  2:132402/1‑135 (MQ=255)
                                                                                                                           aTGTCGCCACGCGACGGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATCACCTGCACCGACGGTGTccacc          <  5:111835/135‑1 (MQ=255)
                                                                                                                                  cACGCGCCTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCAAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATCCCCTGCACCGACGGTGTCCACCACGCGGc   >  2:353588/1‑135 (MQ=255)
                                                                                                                                   aCGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATCACCTGCACCGCCGGTGTCCACCCCGCGGCt  >  2:241454/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                                      
GGCTTTGCATGGTTTGTTTCTCCTGAGCTTGTTGTTTTTGTTGCAACAGGTGATTCGGGATCGGCTCATGCGCTTCTGCGCAGGTCGTAGGCCTCCAGCTCATGGCGCAGCGGCAGCCCTTCCATGTCGCCACGCGACTGCACGGCACGGCTGCCGCACCAGTTGCCGCGCGCTACCGCCTCGGCTACCGGACGGCCCTCCAGTAGCGCGCTGAGCACACCGACGGCAAAGGCATCACCTGCACCGACGGTGTCCACCACGCGGCT  >  ACB122/3816231‑3816496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑