Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,818,237 C→T 8.2% G289D (GGC→GAC)  kdgR_1 ← HTH‑type transcriptional regulator KdgR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,818,2370CT 8.2% 147.7 / 3.8 49G289D (GGC→GAC) kdgR_1HTH‑type transcriptional regulator KdgR
Reads supporting (aligned to +/- strand):  ref base C (24/21);  new base T (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

CGCGGATGATCAGGTTGGCCTTGAAGTCGATGCGCCGGGCTGCGCCGCTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTGAAGATGGCTTGCGG  >  ACB122/3818103‑3818370
                                                                                                                                      |                                                                                                                                     
cgcgGATGATCAGGTTGGCCTTGAAGTCGATGCGCCGGGCTGCGCCGCTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGc                                                                                                                                       >  7:302261/1‑135 (MQ=255)
   ggATGATCAGGTTGGCCTTGAAGGCGATGCGCCGGGCTGCGCCGCTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGa                                                                                                                                    <  2:292913/135‑1 (MQ=255)
     atgaaCAGGTAGGCCTTGAAGTCGATGCGCCGGGCTGCGCCCCTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTCGGCTGGGCCAGTGCGGTCATGCCTTTgacgac                                                                                                                                   >  2:329691/1‑134 (MQ=255)
           aGGTTGGCCTTGAAGTCGATGCGCCGGGCTGCGCCGCTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAggg                                                                                                                             >  5:148189/1‑134 (MQ=255)
                   gttGACGTCGAGGCGCCGGGCGGCGCCGCTGGCGCCGCGCAAGCGACCGAGCTGGCTTTCGACGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTc                                                                                                                    <  5:266059/134‑1 (MQ=255)
                     tGAAGTCGATGCGCCGGGCTGCGCCGCTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGa                                                                                                                  >  5:140417/1‑135 (MQ=255)
                               gcgcCGGGCTGCGCCGCTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAg                                                                                                        >  1:116381/1‑135 (MQ=255)
                               gcgcCGGGCTGCGCCGCTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAg                                                                                                        >  3:249800/1‑135 (MQ=255)
                               gcgcCGGGCTGCGCCGCTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCCAg                                                                                                        >  2:365690/1‑135 (MQ=255)
                                  ccGGGCTGCGCCGCTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGc                                                                                                     <  3:71371/135‑1 (MQ=255)
                                             cgcTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCGGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCg                                                                                           >  4:167849/1‑134 (MQ=255)
                                                tggcgccgcGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACAt                                                                                       <  8:116152/132‑1 (MQ=255)
                                                  tCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCg                                                                                     >  4:90359/1‑135 (MQ=255)
                                                          cAAGCGCCCGAGCAGGCTTGCGCAGGCCGCCACGCCGATACGCGCGGTGGGCTGGGCCATTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCTATGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAg                                                                             <  2:64263/135‑1 (MQ=255)
                                                               gACGGAGCCGGCGTGGGACGGCCGCCACGCCGATACGCTCGGTGGGCGGGGCCAGGGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGGCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTgc                                                                        <  1:189280/135‑1 (MQ=255)
                                                                   gAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGccc                                                                    >  4:12866/1‑135 (MQ=255)
                                                                     gCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCgg                                                                  <  4:62898/135‑1 (MQ=255)
                                                                         gCTTTCCATGGCCGCCACGCCGAGAGGCTCGGGGGGCTGGGCGAGTGCGGTGATGCCTTTGCCGACCAAGGGGTCCCAGACGAGGTCGTCGAGCGCGATCAGCCCGACATGGGCAACCAGGTTGCGCCCGGCCTc                                                              <  2:358774/135‑1 (MQ=255)
                                                                                     ggcccagCCGATACGCGCGGTGGGCTGGGCCAGTGCGGTGAGGCCTTGGCCGCCCAAGGGGTACCAGACGTGGTCGTCGAGGGCGAGCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCg                                                  <  5:301813/129‑1 (MQ=255)
                                                                                     cgccACGCGGATACGCGCGGTGGGCTGGGCCAGGGCGGTGAGGCCTTTCCCGACCAAGGCGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTCCGCCCGGCCTCGAGCAGCGCCCg                                                  <  1:365690/135‑1 (MQ=255)
                                                                                     cgccACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCg                                                  <  4:301949/135‑1 (MQ=255)
                                                                                        cACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGt                                               <  4:15478/135‑1 (MQ=255)
                                                                                          cGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGa                                             >  3:88946/1‑135 (MQ=255)
                                                                                                            gctgggcCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGc                           >  5:201015/1‑135 (MQ=255)
                                                                                                            gctgggcCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGc                           >  2:261475/1‑135 (MQ=255)
                                                                                                            gctgggcCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGc                           >  3:178675/1‑135 (MQ=255)
                                                                                                             ctgggcCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGcc                          <  8:115443/135‑1 (MQ=255)
                                                                                                              tgggcCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCg                         <  2:116381/135‑1 (MQ=255)
                                                                                                                ggcCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGt                        >  2:135326/1‑134 (MQ=255)
                                                                                                                  ccAGTGCGGTGATGCCTTTGCCGACCAAGGTGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTTGCAACGCCGTTGa                     <  8:302261/135‑1 (MQ=255)
                                                                                                                  ccAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGtt                       >  4:224105/1‑133 (MQ=255)
                                                                                                                       gCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTg                <  2:282481/135‑1 (MQ=255)
                                                                                                                         ggTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCTGCCTCGAGCAGCGCCCGGCTGACCGCCAAGGTGGCAACGCCGTTGAAGGt                 >  6:166220/1‑132 (MQ=255)
                                                                                                                         ggTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGt                 >  8:362078/1‑132 (MQ=255)
                                                                                                                         ggTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGt                 >  3:86130/1‑132 (MQ=255)
                                                                                                                              tGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTGAAGATgg         >  5:176080/1‑135 (MQ=255)
                                                                                                                               gCCTTTGCCGACCAAGGGGTACCAGTCGAGGGCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTGAAGATGGc        <  3:370637/135‑1 (MQ=255)
                                                                                                                               gCCTTGGCCGACCATGGGGAACCAGGGGTGGTCGGCGAGGGCGATCAGCCCGACTTCGGCAACCAGGTTGCGCCCGGCCTCTAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTGAAGATGGc        <  4:198950/135‑1 (MQ=255)
                                                                                                                                   ttGTCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGc                                                                                   <  5:277168/56‑1 (MQ=255)
                                                                                                                                   ttGTCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGc                                                                                   <  5:282031/56‑1 (MQ=255)
                                                                                                                                   ttGTCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGc                                                                                   >  6:277168/1‑56 (MQ=255)
                                                                                                                                   ttGTCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGc                                                                                   >  6:282031/1‑56 (MQ=255)
                                                                                                                                   ttGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCACCCAGGTTGCGCCCGGCCTCGAGCAGCTCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTGAAGATGGCTTGc    >  8:248595/1‑135 (MQ=255)
                                                                                                                                   ttGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGCCCGCCAAGGTGGCAGCGCCGTTGAAGGTGAAGATGGCTTGc    >  8:152072/1‑135 (MQ=255)
                                                                                                                                   ttGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCg                         >  6:53388/1‑114 (MQ=255)
                                                                                                                                   ttGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCg                         <  5:53388/114‑1 (MQ=255)
                                                                                                                                   ttGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTGAAGATGGc        <  3:90257/131‑1 (MQ=255)
                                                                                                                                   ttGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTGAAGATGGc        >  4:90257/1‑131 (MQ=255)
                                                                                                                                   ttGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTGAAGATGGCTTGc    >  3:238738/1‑135 (MQ=255)
                                                                                                                                     gCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTGAAGATGGCTTGCgg  >  1:181206/1‑135 (MQ=255)
                                                                                                                                     gCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTGGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTGAAGATGGCTTGCg   <  7:190525/134‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
CGCGGATGATCAGGTTGGCCTTGAAGTCGATGCGCCGGGCTGCGCCGCTGTCGCCGCGCAAGCGACCGAGCAGGCTTTCGAAGGCCGCCACGCCGATACGCTCGGTGGGCTGGGCCAGTGCGGTGATGCCTTTGCCGACCAAGGGGTACCAGTCGAGGTCGTCGAGGGCGATCAGCCCGACATCGGCAACCAGGTTGCGCCCGGCCTCGAGCAGCGCCCGGGTGACCGCCAAGGTGGCAACGCCGTTGAAGGTGAAGATGGCTTGCGG  >  ACB122/3818103‑3818370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑