Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,944,256 T→G 26.5% E142D (GAA→GAC)  CJ019_03729 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,944,2560TG26.5% 62.4 / 3.8 44E142D (GAA→GAC) CJ019_03729hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (5/26);  new base G (11/2);  total (16/28)
Fisher's exact test for biased strand distribution p-value = 3.28e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGTTGAGTTGACCCACGCCGTCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCGATGCCCTGCTGGATGGCCCGCGCCAGGCTGC  >  ACB122/3944130‑3944389
                                                                                                                              |                                                                                                                                     
cTGGTTGAGTTGACCCACGCCGTCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCg                                                                                                                                 <  7:297546/133‑1 (MQ=255)
    ttgagttgaCCCACGCCGTCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAg                                                                                                                           <  4:364365/135‑1 (MQ=255)
       agttgaCCCACGCCGTCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCg                                                                                                                         <  1:5039/134‑1 (MQ=255)
       agttgaCCCACGCCGTCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCg                                                                                                                         <  7:234275/134‑1 (MQ=255)
         ttgaCCCACGCCGTCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGtt                                                                                                                       >  8:119246/1‑134 (MQ=255)
             cccACGCCGTCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTgag                                                                                                                   <  7:320047/134‑1 (MQ=255)
                 cGCCGTCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAgg                                                                                                              <  4:112453/135‑1 (MQ=255)
                 cGCCGTCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAgg                                                                                                              <  3:302836/135‑1 (MQ=255)
                     gTCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGGTGATCGGCCAAGCGGTTGTGATAGGCggg                                                                                                          >  6:180035/1‑134 (MQ=255)
                      tCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTg                                                                                                         <  5:85204/135‑1 (MQ=255)
                                cggcacgCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCt                                                                                               <  7:329675/135‑1 (MQ=255)
                                   cccgcacCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCCGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGGTGCTCGGCGACACGCTTGGGATCGGCGGTGAGGGGGCGCgggg                                                                                            >  6:214910/3‑134 (MQ=255)
                                   cacgcacCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGGCACGCGTTTGTGTTAGGGGGGGTGGTGGGGCTGGt                                                                                            >  2:271799/1‑135 (MQ=255)
                                   cacgcacCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGGTGCTCGGCCAAGCCGTTGAGATTGGCGGTGGGGTTGGGCTGGt                                                                                            >  4:127443/1‑135 (MQ=255)
                                   cacgcacCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGGTGAAAGGCCCAGCGTTTGAGATAGGGGGTGTGGGTGGGCAGGt                                                                                            >  8:347051/1‑135 (MQ=255)
                                   cacgcacCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGGTCATCGGGCAAGCGTTTGAGCTAGGCGGGGAGGGTGCGCgggg                                                                                            >  8:310303/1‑134 (MQ=255)
                                     cgcacCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGCGCAGCCGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCCGTCATCGGCCCAACCTTTGAGAATGGGGGTCAGGTTGCGCGGGTCg                                                                                          >  4:120400/1‑135 (MQ=255)
                                         ccaccTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCa                                                                                      <  7:52135/135‑1 (MQ=255)
                                          caccTGCCGGGGTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCTCGCCCTCCTCCACAGCAGGGTCGCGTTCATCGGCCATGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGccta                                                                                     <  3:120400/135‑3 (MQ=255)
                                          caccTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCaa                                                                                     <  7:347051/135‑1 (MQ=255)
                                                 ccGGCTTCGGCCAGCGGACCGCTCGCGTGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGt                                                                              <  1:255514/135‑1 (MQ=255)
                                                     cTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGa                                                                          <  8:45594/135‑1 (MQ=255)
                                                                    cGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGGTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCg                                                           <  7:375492/135‑1 (MQ=255)
                                                                                    cGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGGTCCTCGGCGAATCGGGTGGGGTATGCGGTGGGGTTTGGGTGGTCGGGCCAGGTGCCTTCCCAGCGTGGCCTGGCCGGGGCGGGGGCATTGcc                                           >  4:168377/1‑135 (MQ=255)
                                                                                         cAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGGTGATCGGCCCAGCGGTTGAGATAGGCGGGGGGGGTGGGCGGGTCGGGCAAGGTCAGTTGGAAGCGTGGGCTGGGCGGGGCGCGGCGATTGCccgccg                                      >  3:279668/1‑135 (MQ=255)
                                                                                         cAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGGTGATAGGGCAAGCGGTTGAGATAGGGGGGGTGGTTGCGCTGGTGGGCCAAGGTCAGTTAGCAGCGTGGGATGGGCGGGGGGAGGCGATTTCCAGCCg                                      >  6:120694/1‑135 (MQ=255)
                                                                                           aGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCggg                                    <  5:120694/135‑1 (MQ=255)
                                                                                              cgcAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCg                                 <  7:119246/135‑1 (MQ=255)
                                                                                              cgcAGCACGCCCTCCGCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCg                                 <  3:35768/135‑1 (MQ=255)
                                                                                                cagcaCGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGATGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCGAt                               <  1:134606/135‑1 (MQ=255)
                                                                                                   caCGCCCTCCTCCACACCAGGGTCGCGGTGATCGGCCCACCGTGTGAGATAGGCGGGGAGGGTGCGCTGGTCGGGCAAGGTCCGGTGGAAGCGGGGGCTGGGCGGGGCGCGGCGCTTGCCCGCCGGGTCgcggcc                            >  8:349306/1‑130 (MQ=255)
                                                                                                   caCGCCCTCCTCCACACCAGGGTCGCGGTCATCGGCCCAGCGTTTGAGATAGGCGGTGAGGTTGGGCTGGTCGGCCAAGGTCAGTTCGTAGCGTGGCATGGGCGGGTCGAGGCGATTGCCAGCCGGGTCGCTggc                            >  8:109498/1‑133 (MQ=255)
                                                                                                        cctcctCCACACCCGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGACGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCGATGCCctgct                       <  7:31713/135‑1 (MQ=255)
                                                                                                        cctcctCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCGATGCCctgct                       <  4:156767/135‑1 (MQ=255)
                                                                                                            ctccACCCCAGGGTCGCGTTCATCGGCCAAGCGGTTGAGATAGGCGGTGAGGTTGCGCTGGGCGGCCAAGGGCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCGATGCCCTGCTGGAt                   <  5:214910/135‑1 (MQ=255)
                                                                                                            ctccACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCGATGCCCTGCTGGAt                   <  5:292032/135‑1 (MQ=255)
                                                                                                            ctccACACCAGGGTCGCGTTCAGCGGCCAAGCGTTTGAGATAGGCGGTTAGGTTGCGCTGGGCGGCCAAGGTCAGTGCGAAGCGTGGCATGGCCGTGTCGAGGCGATTGCCAGCCGGGTCGAGGCCCTGCTGGAt                   <  5:318751/135‑1 (MQ=255)
                                                                                                            ctccACACCAGGGTCGCGGTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGGCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGGCGATGCCCTGCTGGAt                   <  2:19130/135‑1 (MQ=255)
                                                                                                                    cAGGGTCGCGTTGATAGGCCCAGCGGTTGAGATATGCGGTGAGGGTGGGGGGGTGGGCCCAGGGGAGGTGGAAGCGTGGCATGGCCGGGGGGAGGCGATTTCCACCCCGGGCGCTGCCCAGCTGGATGGCCcgcc           >  2:95541/1‑134 (MQ=255)
                                                                                                                       ggTCGCGTTCAACGGCCAAGCGTTTGAGATATGCGGTGAGGTTGCGCGTGTCGGGCAATGTCAGTTCGAATCGTGGCATTGCCGGGTCCCGGCCATTGCCACCCCGGTCGCAGCCCAGCTGGATGGCCCCCGccc        >  8:95875/1‑134 (MQ=255)
                                                                                                                       ggTCGCGTTAATAGGCCAAGCGGTTGAGATAGGCGGTGAGGGTGGGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGGCGAGGCGATTTCCAGCCGGGTCGATGGCCTGCTGGCTGGCCCGCGCCa        >  8:215885/1‑135 (MQ=255)
                                                                                                                             gTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCGATGCCCTGCTGGATGGCCCGCGCCAGgctgc  <  5:180035/135‑1 (MQ=255)
                                                                                                                             gTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCGATGCCCTGCTGGATGGCCCGCGCCAGgctgc  <  3:135464/135‑1 (MQ=255)
                                                                                                                             gTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCGATGCCCTGCTGGATGGCCCGCGCCAGgctgc  <  2:30694/135‑1 (MQ=255)
                                                                                                                              |                                                                                                                                     
CTGGTTGAGTTGACCCACGCCGTCTTCCAGCACGGCACGCACCACCTGCCCGGCTTCGGCCAGCGGACCGCTCGCGGGCAGCAGCGTGCCAAGGCGCAGCACGCCCTCCTCCACACCAGGGTCGCGTTCATCGGCCAAGCGTTTGAGATAGGCGGTGAGGTTGCGCTGGTCGGCCAAGGTCAGTTCGAAGCGTGGCATGGCCGGGTCGAGGCGATTGCCAGCCGGGTCGATGCCCTGCTGGATGGCCCGCGCCAGGCTGC  >  ACB122/3944130‑3944389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑