Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,957,251 G→A 100% P133L (CCG→CTG)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,957,2510GA96.9% 224.1 / ‑2.1 65P133L (CCG→CTG) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (28/34);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGG  >  ACB122/3957123‑3957381
                                                                                                                                |                                                                                                                                  
cGTGGGGGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCgg                                                                                                                              <  1:192549/135‑1 (MQ=255)
  tGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCgggg                                                                                                                            <  4:349155/135‑1 (MQ=255)
   gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGt                                                                                                                           >  5:178114/1‑135 (MQ=255)
   gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGt                                                                                                                           >  5:152741/1‑135 (MQ=255)
   gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGt                                                                                                                           >  6:5642/1‑135 (MQ=255)
   gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGt                                                                                                                           >  1:204812/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGtt                                                                                                                          <  3:161563/134‑1 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                         >  7:73414/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                         >  1:63531/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                         >  2:160743/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                         >  8:87324/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                         >  3:334164/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCAAGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                         >  8:247126/1‑135 (MQ=255)
          cAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTg                                                                                                                    <  8:128895/135‑1 (MQ=255)
           aGGTAGCCGGGGGCCGCGCAGTGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGGCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTg                                                                                                                    <  3:6575/134‑1 (MQ=255)
           aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTg                                                                                                                    >  3:94566/1‑134 (MQ=255)
           aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGc                                                                                                                   <  4:285892/135‑1 (MQ=255)
           aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGc                                                                                                                   <  6:320159/135‑1 (MQ=255)
                     gggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCa                                                                                                         >  1:144279/1‑135 (MQ=255)
                          gcgcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCCGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCg                                                                                                    >  7:18640/1‑135 (MQ=255)
                            gcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTGGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                                  <  5:27276/135‑1 (MQ=255)
                              aGGGGATGCTGAAAATATTTCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCAACGTCCACGGTGATCACCACGTCAAGCCCGTCGGCCAGCAGGTCGGGGTTGCGCTGCGCCAGCGTCAGCGCGATca                                                                                                >  2:196156/1‑135 (MQ=255)
                                         aaaaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                                      >  5:149500/1‑134 (MQ=255)
                                           aaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCTTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTa                                                                                   <  8:7171/135‑1 (MQ=255)
                                            atatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTaa                                                                                  <  6:378751/135‑1 (MQ=255)
                                                                  tCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGccacca                                                            <  4:334164/135‑1 (MQ=255)
                                                                       tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCgg                                                        >  6:97372/1‑134 (MQ=255)
                                                                       tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                                       <  4:182215/135‑1 (MQ=255)
                                                                       tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                                       >  8:285279/1‑135 (MQ=255)
                                                                       tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                                       >  3:82739/1‑135 (MQ=255)
                                                                       tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                                       <  1:196156/135‑1 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACTTCAAGCCCTTCGGCCAGCAGGTCGGTGTTGCGCTGCTACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                  <  7:247126/135‑1 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCTGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                  <  6:178114/135‑1 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                  <  1:160743/135‑1 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                  <  5:228727/135‑1 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                  <  1:40360/135‑1 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                  <  4:224584/135‑1 (MQ=255)
                                                                              ccGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCa                                                <  5:104036/135‑1 (MQ=255)
                                                                                               aCGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTg                                                                                                                    <  6:378181/50‑1 (MQ=255)
                                                                                               aCGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTg                                                                                                                    >  5:378181/1‑50 (MQ=255)
                                                                                                cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                              <  8:207242/135‑1 (MQ=255)
                                                                                                cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                              <  6:136230/135‑1 (MQ=255)
                                                                                                cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                              <  1:213441/135‑1 (MQ=255)
                                                                                                cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                              <  5:97372/135‑1 (MQ=255)
                                                                                                cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                              <  4:270540/135‑1 (MQ=255)
                                                                                                cGGTGATCACCACGTCATGCCCTGCGGCCAGCAGGGCGGGGTGGCGCTGCGACAGCGTCAGCTCGATCACCCCTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                              <  6:302410/135‑1 (MQ=255)
                                                                                                cGGGGAGCACCACGTCAGGCCCTTCGGCCAGCAGGGCGGGGGTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAACGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                              <  1:155038/135‑1 (MQ=255)
                                                                                                        accacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTg                       >  8:128145/1‑134 (MQ=255)
                                                                                                         ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc                      >  5:61105/1‑134 (MQ=255)
                                                                                                         ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc                      >  5:147585/1‑134 (MQ=255)
                                                                                                         ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc                      >  4:108749/1‑134 (MQ=255)
                                                                                                         ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc                      >  1:371997/1‑134 (MQ=255)
                                                                                                         ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc                      >  2:327363/1‑134 (MQ=255)
                                                                                                         ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAT‑‑CCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCaaa                   >  1:163498/1‑135 (MQ=255)
                                                                                                              tCATGCCCTTCGGCCAGCCGGTCGGGGGTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc                <  6:327729/135‑1 (MQ=255)
                                                                                                              tCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc                <  2:204812/135‑1 (MQ=255)
                                                                                                              tCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc                <  1:135307/135‑1 (MQ=255)
                                                                                                              tCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc                <  5:115527/135‑1 (MQ=255)
                                                                                                                aTGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCAGCAGCAACAGCCCCCTGCCGTACGGGCAGTGCAAAc                  >  5:50012/1‑131 (MQ=255)
                                                                                                                  gCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGAt            >  8:94520/1‑135 (MQ=255)
                                                                                                                     cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc         <  4:94566/135‑1 (MQ=255)
                                                                                                                     cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc         <  5:343977/135‑1 (MQ=255)
                                                                                                                          gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGcc                                                                    <  5:168629/71‑1 (MQ=255)
                                                                                                                          gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGcc                                                                    >  6:168629/1‑71 (MQ=255)
                                                                                                                           ccAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAg   >  3:367632/1‑135 (MQ=255)
                                                                                                                            cagcagGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAgg  <  8:276880/135‑1 (MQ=255)
                                                                                                                                |                                                                                                                                  
CGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGG  >  ACB122/3957123‑3957381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑