Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,985,770 G→A 7.3% A315V (GCC→GTC)  CJ019_03763 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,985,7700GA 7.3% 179.6 / 3.5 55A315V (GCC→GTC) CJ019_03763hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base A (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

CATTTGCTCGCGGGCCTGACGGTTACTGGTAGCCGGCCGTTCGATACCTGCGCGGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGTGCTGCCATTTGTTCTGGTTGTTGGTGATGCGGTTGTTGACGTTGAGGTTGTTG  >  ACB122/3985640‑3985901
                                                                                                                                  |                                                                                                                                   
cATTTGCTCGCGGGCCTGACGGTTACTGGTAGCCGGCCGTTCGATACCTGCGCGGTCCCTCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGCCCGCGGTGCGGTGCGTGGcccc                                                                                                                                 >  5:142579/1‑135 (MQ=255)
 aTTTGCTCGCGGGCCTGACGGTTACTGGTAGCCGGCCGTTCGATACCTGCGCGGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCg                                                                                                                                >  1:212317/1‑135 (MQ=255)
    tGCTCGCGGGCCTGACGGTTACTGGTAGCCGGCCGTTCGATACCTGCGCGGGCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTc                                                                                                                              <  5:266367/134‑1 (MQ=255)
     gCTCGCTGGCCTGACGGTTACTGGTAGCCGGCCGTGGGATACCTGCGCGGTGCATCGCTGCCCTGGCTTTGTCGCGGGGCTGGGGGCGTGGGGGGTCGGCGGCGCGGGACGCGGGGCGGTGGCTGGGACCgggcc                                                                                                                            >  1:193242/1‑131 (MQ=255)
      gtCGCGGGCCTGCCGGTGACGGGTAGCCGGCCGGTCGATACCTGCGCGGTCCATCGATGCCCTGGCTGTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTcc                                                                                                                             <  3:228776/132‑1 (MQ=255)
             gCCTGACGGTTACTGGTAGCCGGCCGTTCGATACCTGCGCGGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTggcgg                                                                                                                    <  7:357539/135‑1 (MQ=255)
                         cTGGTAGCCGGCCGTTCGATACCTGCGCGGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATActgctg                                                                                                         <  5:370317/134‑1 (MQ=255)
                            gTAGCCGGCCGTTCGATACCTGCGCGGTCCATCGAGGCCCTGGCGTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGCCCGCGGGGCGCGGAGTGGCACCGGCCAGTTGGCGGCCCTACTGCTGCCgg                                                                                                     >  6:251045/1‑135 (MQ=255)
                                 cggccgTTCGATACCTGCGCGGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGCACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCCGACTGCTGCCGGCTg                                                                                                  >  7:367912/1‑133 (MQ=255)
                                  ggccgTTCGATAACTGCGCGGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGGGTCGTCGCCGCGGCACGCGGGGGGCTGAGTGGCACCGTCCCGTTGGCGGCCCTTCTGCGGTCGGCTGcca                                                                                               >  5:383041/1‑133 (MQ=255)
                                     cgTGCGATCCCTGCGCGGGCCAGCGCTGCCCTGGCTTGGGCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGGCCAGTTGGCGGCCATACTGCTGACGGCTGCGagag                                                                                            <  6:32682/135‑1 (MQ=255)
                                       ttCGATACCTGCGCGGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTc                                                                                          <  1:91481/135‑1 (MQ=255)
                                       ttCGATACCTGCGCGGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTc                                                                                          <  3:294077/135‑1 (MQ=255)
                                       ttCGATACCTGCGCGGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGGCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTc                                                                                          <  2:212317/135‑1 (MQ=255)
                                         cgctACCTGCGCGGTCCATCGATGCCCTGGCGTTGGCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTcgc                                                                                        <  2:345906/132‑1 (MQ=255)
                                                  cgcgGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGACACCGTCCAGTTGGCGGCCATACTGCTGCCGGCTGCGAGAGTCGCGGTATGGca                                                                               >  6:21359/1‑135 (MQ=255)
                                                  cgcgGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGACACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGca                                                                               >  6:119913/1‑135 (MQ=255)
                                                    cgGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTg                                                                                                  >  3:78338/1‑114 (MQ=255)
                                                    cgGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTg                                                                                                  <  4:78338/114‑1 (MQ=255)
                                                    cgGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGagag                                                                                            >  3:85618/1‑120 (MQ=255)
                                                    cgGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGagag                                                                                            <  4:85618/120‑1 (MQ=255)
                                                    cgGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGcacc                                                                             >  3:188980/1‑134 (MQ=255)
                                                    cgGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGcaca                                                                             >  5:321756/1‑135 (MQ=255)
                                                     ggTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCacac                                                                            <  8:301341/135‑1 (MQ=255)
                                                           tCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGtctc                                                                      <  5:290745/135‑1 (MQ=255)
                                                             gATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGc                                                                                >  6:19963/1‑123 (MQ=255)
                                                             gATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGc                                                                                <  5:19963/123‑1 (MQ=255)
                                                              aTGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGt                                                                   <  4:62078/135‑1 (MQ=255)
                                                              aTGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGGCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGt                                                                   <  6:267391/135‑1 (MQ=255)
                                                                  ccTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGc                                                               >  4:317865/1‑135 (MQ=255)
                                                                   cTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCg                                                              <  2:265296/135‑1 (MQ=255)
                                                                    tGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCCCACCGTCTCGGTTGgggg                                                             >  3:158380/1‑135 (MQ=255)
                                                                    tGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCCCACCGTCTCGGTTGGCg                                                              >  7:358967/1‑134 (MQ=255)
                                                                    tGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCg                                                              >  7:90937/1‑134 (MQ=255)
                                                                    tGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCg                                                              >  4:309298/1‑134 (MQ=255)
                                                                          tggcgcGGGCCGGGGCCCGTGGGGCGGCGGCGCCGCGGACCGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATtgt                                                       <  5:251045/130‑1 (MQ=255)
                                                                           gTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGGCTCGGTTGGCGGAATtgtg                                                      >  3:290656/1‑135 (MQ=255)
                                                                             ctcGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGtgc                                                     <  6:153190/134‑1 (MQ=255)
                                                                              tcGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGtgctg                                                   <  4:322655/135‑1 (MQ=255)
                                                                                         cccGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGTGCTGCCATTTGTTCt                                        >  2:10351/1‑135 (MQ=255)
                                                                                         cccGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGGTGGCGGAATTGTGCGGCCATTTGttgt                                        >  4:8024/1‑133 (MQ=255)
                                                                                         cccGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGGCGCGGTTGGCGGAATTGTGGTGCCATTTGTTCt                                        >  5:98236/1‑135 (MQ=255)
                                                                                          ccGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTc                                                                                          >  4:175406/1‑84 (MQ=255)
                                                                                          ccGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTc                                                                                          <  3:175406/84‑1 (MQ=255)
                                                                                          ccGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGTGCTGCCATTTGTTCTg                                       >  7:97766/1‑135 (MQ=255)
                                                                                               gggCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGGCTCGGGTGGCGGAATTGTGCTGCCATTTGTTCTGgttgt                                  >  2:331192/1‑135 (MQ=255)
                                                                                                ggCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGc                                                                                >  7:175445/1‑88 (MQ=255)
                                                                                                ggCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGc                                                                                <  8:175445/88‑1 (MQ=255)
                                                                                                    tcgtcgCCGCGGAACGCGGTGCGCTGAGTGACACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGTGCTGCCATTTGTTCTGGTTGTTGGTg                             <  5:21359/135‑1 (MQ=255)
                                                                                                    tcgtcgCCGCGGAACGCGGTGCGCTGAGTGACACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGTGCTCCCATTTGTTCTGGTTGTTGGTg                             <  5:119913/135‑1 (MQ=255)
                                                                                                         gccgcGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGTGCTGCCATTTGTTCTGGTTGTTGGTGATGCg                        >  4:332490/1‑135 (MQ=255)
                                                                                                          ccgcGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGTGCTGCCATTTGTTCTGGTTGTTGGTGATGCgg                       <  6:321756/135‑1 (MQ=255)
                                                                                                                  cgcgGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGTGCTGCCATTTGTTCTGGTTGTTGGTGATGCGGTTGTTGAc               <  4:365348/135‑1 (MQ=255)
                                                                                                                      gTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGTGCTGCCATTTGTTCTGGTTGTTGGTGATGCGGTTGTTGACGTTg           <  8:90937/135‑1 (MQ=255)
                                                                                                                               gTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGTGCTGCCATTTGTTCTGGTTGTTGGTGATGCGGTTGTTGACGTTGAGGttgttg  <  8:97766/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                   
CATTTGCTCGCGGGCCTGACGGTTACTGGTAGCCGGCCGTTCGATACCTGCGCGGTCCATCGATGCCCTGGCTTTGTCTCGGGCCTGGGCCCGTTGGGCGTCGTCGCCGCGGAACGCGGTGCGCTGAGTGGCACCGTCCAGTTGGCGGCCATACTGCTGACGGCTGCGAGAGTCGCGGTATGGCACACCGTCTCGGTTGGCGGAATTGTGCTGCCATTTGTTCTGGTTGTTGGTGATGCGGTTGTTGACGTTGAGGTTGTTG  >  ACB122/3985640‑3985901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑