Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,094,877 A→G 6.7% I115T (ATC→ACC)  CJ019_03856 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,094,8770AG 6.7% 177.3 / 3.9 60I115T (ATC→ACC) CJ019_03856hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/29);  new base G (2/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

TTGCCCAAGTGCCCTGTACCAGCAGCACTCCGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCAAGGCCCCTGCAGG  >  ACB122/4094752‑4095005
                                                                                                                             |                                                                                                                                
ttGCCCAAGTGCCCTGTACCAGCAGCACTCCGCACAGGATCAGCACCTCGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGcc                                                                                                                         <  6:50428/135‑1 (MQ=255)
ttGCCCAAGTGCCCTGTACCAGCAGCACTCCGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGcc                                                                                                                         <  6:358055/135‑1 (MQ=255)
   cccAAGTGCCCTGTACCAGCAGCACTCCGCACAGGATCATCACCACGCCGGCCACCCTGGACAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGcccc                                                                                                                       <  6:120540/134‑1 (MQ=255)
   cccAAGTGCCCTGTACCAGCAGCACTCCGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGcccc                                                                                                                       <  1:370301/134‑1 (MQ=255)
    ccAAGTGCCCTGTACCAGCAGCACTCCGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGc                                                                                                                     >  5:101194/1‑135 (MQ=255)
       aGTGCCCTGTACCAGCAGCACTCCGCACAGGATCAGCACCACGCCGGCCAGCCTGTCCAGGCTGAGCGGCTTGGCACCCAGCCCCATCTCCCCGTAGTGGTCGTCAAGCACTGCCGTGATGATCTGCCCCGcgac                                                                                                                  <  2:41025/135‑1 (MQ=255)
       aGTGCCCTGAACCAGCAGCACTCCGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGccac                                                                                                                  <  2:134307/135‑1 (MQ=255)
              tGTACCAGCAGCACTCCGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGAACAGCCCCCgcaccaccacg                                                                                                           >  8:73265/1‑133 (MQ=255)
                tACCAGCAGCACTCCGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTa                                                                                                          >  8:277473/1‑134 (MQ=255)
                        gcaCTCCTCACAGGATCAGCACAACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTACTGCTCGGCAACCACTGCCGAGATTATCTTCCCCCCCGCCCACAGTACCACGAAc                                                                                                  >  3:367327/1‑134 (MQ=255)
                        gcaCTCCGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGccacca                                                                                                                <  6:332127/120‑1 (MQ=255)
                        gcaCTCCGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGccacca                                                                                                                >  5:332127/1‑120 (MQ=255)
                        gcaCTCCGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAAcc                                                                                                 <  8:124889/135‑1 (MQ=255)
                             ccGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCctgct                                                                                            >  4:17025/1‑135 (MQ=255)
                               gCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCtgctgc                                                                                          <  5:340658/135‑1 (MQ=255)
                               gCACAGGATCAGCACCACGCCGGCCACCCGGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCtgctgc                                                                                          <  6:341574/135‑1 (MQ=255)
                                  caGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCCGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGGTGATCTGCCCCGCCCCCCCCAGTACCAGGCACCCTGCTgcgc                                                                                        >  6:337787/1‑134 (MQ=255)
                                  caGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCCTCACCCCGTAGTGGTCGGCAAGCACTGCCGTGGTGATCTGCCCCGCCCCCCCCAGTACCCGGCACCCAGCAgcgc                                                                                        >  6:332161/1‑134 (MQ=255)
                                        cAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCCCCCCGTAGTGGTCGGCAAGCACTGCCGTGCTGATCTGCCCCGCCCCCACCCGTCCCAGGAACCCAGCAGCGCCaact                                                                                   >  6:237351/1‑131 (MQ=255)
                                         aGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCt                                                                                                                            >  3:31548/1‑91 (MQ=255)
                                         aGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCt                                                                                                                            <  4:31548/91‑1 (MQ=255)
                                         aGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTa                                                                                >  4:117994/1‑135 (MQ=255)
                                                  ccGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCCCCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGt                                                                       >  4:303775/1‑135 (MQ=255)
                                                  ccGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCACCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGt                                                                       >  8:247882/1‑135 (MQ=255)
                                                   cGGCCACCCTGGCCAGGCTGAGCGGCTTGCCCCCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCCCCACCACTACCAGGAACCCTGCTGCGCCAAGTTTACGGGTGAGTg                                                                      >  2:312717/1‑135 (MQ=255)
                                                   cGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTTCCCCGCCACCCCCAGTACCCCGCACCCTGCTGCGCCCAGGTTTTGGGGGAGTg                                                                      >  2:370776/1‑135 (MQ=255)
                                                   cGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCCCCCCCAGTACCAGGAACCCTGCTGCGCCCAGTTTAGGGGTGAGTg                                                                      >  5:189852/1‑135 (MQ=255)
                                                   cGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCCCCAGGACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTg                                                                      >  2:313769/1‑135 (MQ=255)
                                                       caccgcggCCAGGCTGAGCGGCGTGCCACCCAGCCCCATCACCCCGTAGTGGGCGGCAAGCACTGCCGTGACGATCTGCCCCGCCCCCACCAGTACCAGGAACCCTGCTGCGCCAAGGGTAGGGGTGAGTgcggc                                                                  <  5:38940/129‑1 (MQ=255)
                                                         cccTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTgcggcgg                                                                <  4:300932/135‑1 (MQ=255)
                                                         cccTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTgcggcgg                                                                <  4:263275/135‑1 (MQ=255)
                                                         cccTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTgcggcgg                                                                <  3:17025/135‑1 (MQ=255)
                                                         cccTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGGTTAGGGGTGAGGgcggcgg                                                                <  8:125101/135‑1 (MQ=255)
                                                                cAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGc                                                         <  4:9343/135‑1 (MQ=255)
                                                                           gCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTg                                              >  4:241356/1‑135 (MQ=255)
                                                                                 cACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGa                                        <  7:52652/135‑1 (MQ=255)
                                                                                       gCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGc                                  <  1:312717/135‑1 (MQ=255)
                                                                                       gCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGc                                  <  3:278984/135‑1 (MQ=255)
                                                                                         cccATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGcca                                >  7:14281/1‑135 (MQ=255)
                                                                                           cATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCGCGCCACCACCAGTACCAGGAACCCGGCTGCGCCAACTTTATGGGTGAGTGCGGCGGCGCCGCCTACATACATTGCCCCGACCACGccccc                              >  8:344118/1‑135 (MQ=255)
                                                                                                ccccGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATcc                         <  3:272377/135‑1 (MQ=255)
                                                                                                  ccGTAGTGTTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCAGCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCTATCcac                       <  7:344118/135‑1 (MQ=255)
                                                                                                     tAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCtacat                                                    >  8:221786/1‑103 (MQ=255)
                                                                                                     tAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCtacat                                                    <  7:221786/103‑1 (MQ=255)
                                                                                                        tGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCCCCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCCCCAATCCACAcccac                 >  5:104813/1‑135 (MQ=255)
                                                                                                           tCGGCAAGCACTGCCGTGGTGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCaa              <  5:337787/135‑1 (MQ=255)
                                                                                                           tCGGCAAGCACTGCCGTGGTGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCaa              <  5:332161/135‑1 (MQ=255)
                                                                                                           tCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAAGCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCaa              <  7:138101/135‑1 (MQ=255)
                                                                                                           tCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCaa              <  7:160913/135‑1 (MQ=255)
                                                                                                             ggCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACCTTGCCCCGAGCACGCCACCAATCCACAACCACCAAgg            >  3:281241/1‑135 (MQ=255)
                                                                                                             ggCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCAAgg            >  6:186501/1‑135 (MQ=255)
                                                                                                              gCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCAAgg            <  4:360101/134‑1 (MQ=255)
                                                                                                              gCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCAAGGc           >  2:33806/1‑135 (MQ=255)
                                                                                                              gCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCGACATACATTGCCCCGCGCACGCCACCAATCCACAACCCCCAAGGc           >  6:192407/1‑135 (MQ=255)
                                                                                                                aaGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCAAGGccc         >  3:286700/1‑135 (MQ=255)
                                                                                                                aaGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCAAGGccc         >  2:217458/1‑135 (MQ=255)
                                                                                                                  gCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGACCCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGGTACATACATTGCCCCGAGCACGCCCCCAATCCCCAACCACCAAGGCCCCt       >  3:317610/1‑135 (MQ=255)
                                                                                                                  gCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCAAGGCCCCt       <  1:217458/135‑1 (MQ=255)
                                                                                                                         cGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCAAGGCCCCTGCAgg  <  3:298690/133‑1 (MQ=255)
                                                                                                                         cGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCAAGGCCCCTGCAgg  <  3:117994/133‑1 (MQ=255)
                                                                                                                             |                                                                                                                                
TTGCCCAAGTGCCCTGTACCAGCAGCACTCCGCACAGGATCAGCACCACGCCGGCCACCCTGGCCAGGCTGAGCGGCTTGCCACCCAGCCCCATCACCCCGTAGTGGTCGGCAAGCACTGCCGTGATGATCTGCCCCGCCACCACCAGTACCAGGAACCCTGCTGCGCCAAGTTTAGGGGTGAGTGCGGCGGCGCCGGCTACATACATTGCCCCGAGCACGCCACCAATCCACAACCACCAAGGCCCCTGCAGG  >  ACB122/4094752‑4095005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑