| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,135,607 | A→C | 48.9% | Q32P (CAG→CCG) | tgnC → | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,135,607 | 0 | A | C | 48.9% | ‑0.8 / 15.4 | 26 | Q32P (CAG→CCG) | tgnC | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (5/7); new base C (9/4); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
GCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTG > ACB122/4135479‑4135740 | gCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCTg > 4:373517/1‑135 (MQ=255)gCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGAACCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAAACCGGCCACCCCCCAGGCCTg > 7:190459/1‑135 (MQ=255) ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCATCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGATCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCGGCCAGGgcg > 5:185915/1‑135 (MQ=255) cTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCCGCCAGGgcgc > 7:89872/1‑135 (MQ=255) cTCACCGAATACGCCAAGGGGATTGTAGCATGACCCTCGTTCGTTTCCAGATGTGCATCGAAGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCCCCCCCCAGGCCTGGgcg > 2:47632/1‑134 (MQ=255) cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACCGCCTCAACCCGGCCACCGCCCCGGCCGGGGGGCCAcgg > 6:220273/1‑133 (MQ=255) tCCGCCAAGGGTTTTGTTGCAGGACCCTGGTTCGTTTACAGATGTGCATCGACGGCCAATGGCGCGATGCACAGTGCTGCAAGACCTTCGACAGCCTAAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCg < 8:177514/135‑1 (MQ=255) gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCTGGGCGCCACTGCccgccg > 2:237323/1‑135 (MQ=255) ccAAGGGTTTTGTTGCTTGACCCTCGTTCTTTTCAAGATGTCCATCGACGGCAAATGGCGCGATGCCCAGAGCGGAAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGc < 2:94703/135‑1 (MQ=255) cAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCAGGGCGCCACTGCCCgccgcc > 6:24521/1‑135 (MQ=255) cAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCGGCCAGCGCGCAACGGCCCCAccc > 4:374049/1‑132 (MQ=255) tttgttgCATCACCCGCGTTCGTTTCCAGATGTGCAGCGACGCCCAAGGGCGCGCTGCCCAGAGCGGAAACACCTTCGCCAGCCTCAACCCGCCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAg < 8:241233/134‑1 (MQ=255) gttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCCCCAGCCCGACGCCGCTGAAGc > 5:342207/1‑133 (MQ=255) gCATCCCCGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGc < 4:165782/135‑1 (MQ=255) aTCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCCGCGCGCCGCGGGCCGACGCCGAGCCAGCCGAGGTGGGCCTGGCCGGTCACGCCGCCCCGcca > 5:324754/1‑133 (MQ=255) cGACGGCCAATGGCGCGATGCCCAGAGCGGCGAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGCTGCAGCCGCTGTGGAGCTGGCCGTTCAGGCCGCCCCGCGTg > 8:130886/1‑134 (MQ=255) cGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGGAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGc > 2:285739/1‑135 (MQ=255) ccAAGGGCGCGATCCCCGTAGCGCCCAGACCTGCGCCCCCCCCACCCCGCCCACCGCCCTGGCGTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGa < 6:337504/135‑1 (MQ=255) tccccAGCGCGCCAACCCCTCCGACCCCCCCACCCCGCCCCCCCCCCCGGCCGGGCCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 7:30252/133‑1 (MQ=255) cgCCCAGCGCGCCAACCCCTTCGACCGCCCCCCCCCGCCCCCCCCCCCGCCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 5:135354/134‑1 (MQ=255) ccgagCGGAAACACCTTCGCCCGCCACAACCCCCCCCCCGCCCAGCCCTGGCCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGgcgc < 3:373517/133‑1 (MQ=255) gCAAGACCTTCTACAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCa < 8:190459/135‑1 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACCCCCCCCGCCGGGGGGCCCCTCGCCGCCGCCCAGGAACGCGCTCTTGAGCAGGCCCGTCAGGCCGCCCCGCGTGCCGTCGCCAGCAAGGCGGGGCGCCGCCTccaccc > 2:175752/1‑130 (MQ=255) gccaaCCCCCCCGCCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTGAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct < 5:34651/131‑1 (MQ=255) cACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCtgctgct < 1:101055/135‑2 (MQ=255) cAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTg < 4:304553/135‑1 (MQ=255) | GCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTG > ACB122/4135479‑4135740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |