| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,287,814 | T→G | 55.0% | intergenic (+119/‑106) | CJ019_04021 → / → gloA | hypothetical protein/Lactoylglutathione lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,287,814 | 0 | T | G | 55.0% | ‑2.1 / 9.5 | 25 | intergenic (+119/‑106) | CJ019_04021/gloA | hypothetical protein/Lactoylglutathione lyase |
| Reads supporting (aligned to +/- strand): ref base T (3/5); new base G (7/6); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-01 | |||||||||||
GTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCG‑CTTTCATTTGCGCAATCGGCGCACTGCCGTATCA‑TGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGC > ACB122/4287687‑4287929 | gTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTg > 8:297663/1‑135 (MQ=255) aCGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCGGCCGTTGCa > 3:106163/1‑135 (MQ=255) gatgaAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGGGCCCTGCCTTTTCAGGgcg > 6:264660/1‑135 (MQ=255) aTAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCGGCCTTTGGAGGGCG‑CGGTGATTTgcgga > 8:367297/1‑133 (MQ=255) aTAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCGGCCGTTGCAGGGCG‑CGTTCATTTgcgc > 2:31159/1‑134 (MQ=255) tacacaCTTTTTTCACACGCACGGGCCCAGAATCTAAACACAAAACGCACACGCGACCCCATCCCGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCG‑CTTTCATTTGCGCAATCGgc < 3:309432/133‑1 (MQ=255) acTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCAGCCGTTGGAGGGGG‑CGGTAATTTTCGCAATCGgcgccca > 1:216532/1‑132 (MQ=255) cacgcaCTGGCTCATTAACTAAACCCCAAAAGCCCACGCGACCCCATCCCGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCG‑CTTTCATTTGCGCAATCGGCGCACTGCCGTATCA‑t < 7:367297/130‑1 (MQ=255) cGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCGGGCGTTGGAGGGCG‑GGTTGAGTTTCGGAATAGGCGCACGGGCGGTTCA‑TGGGCAGCCGGTCTGgttt > 7:83214/1‑135 (MQ=255) cGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCGGCCGTTGGAGGGCG‑CGTTAATTTTGGCCATAGGCGCACTGCCGTATCA‑TGGGCAGCCGGTGGGtttt > 1:298522/1‑135 (MQ=255) cAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCCGCCGGTTCAGGGGGCCTTTCATTTGGGCACTCGG‑GCCCCGCCCGATCA‑TGGGCACCCCGAGTGtttttgcgg > 2:344892/1‑130 (MQ=255) cAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCAGCCTTTGCAGGGCG‑CTTTCATTTGCGCAATCGGCGCACTGCCCGATCA‑TGGCCCGCCTGTCTGTTTTGcccg > 4:54159/1‑133 (MQ=255) cTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCGGGCGTTGCAGGGGG‑CGTTGAATTTGGCCATCGGCGCCCGGCCGGTTCA‑TGGGCCGCCGGGGGGTTTTTCCGgcgc > 3:135698/1‑135 (MQ=255) aaaGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCAGCCGTTGCAGGGCG‑CGTTCATTTGCGCCAAAGGCGCCCGGCCGGTTAA‑TGGGCCGCCGGTCTGTTTTTCCGGCGccc > 6:200684/1‑135 (MQ=255) cTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCG‑CTTTCATTTGCGCAATCGGCGCACTGCCGTATCA‑TGGCCAGCCTGGCTGTTTTGCCGGCGCCCTGCGcc > 6:362425/1‑135 (MQ=255) acgccaccccaccccCCGCCCCCACAGGCGGGCCTATAATTTCAGCGCCCTGCCTTTGCAGGGCG‑CTTTCATTTGCGCAATCGGCGCACTGCCGTATCA‑TGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGccc < 7:297663/130‑1 (MQ=255) gcgcCCCCAGCCCGCGCCCGCACAGGGGGGCCTAAAATTTCAGCGCCCGCCCTTTGCTGGGCG‑CTTTCATTTGCGCAATCGGCGCACTGCCGTATCA‑TGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCgg < 6:167725/135‑1 (MQ=255) gcgcCCCCAACCCGCCCCCCCACAGGCGGGCCTCTAATTTCCGCCCCCGCCCTTTGCGGGGCG‑CTTTCATTTGCGCAATCGGCGCACTGCCGTATCA‑TGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCgg < 2:23902/135‑1 (MQ=255) gACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCGGCCGTTGCAGGGCG‑CGTTCATTTGCGCAATCGGCGCACGGCCGGATCAGTGGCC‑GCCTGGCTGGTTTTGCGGCGCCCGGCGCCCGGcc > 5:167725/1‑135 (MQ=255) ccATCCCGCCCCCCCACATGCGGGCCTCTAATTCCCGCCCCCGGCCTTGCCGGGGCG‑CTTTCATTTGCGCAATCGGCGCACTGCCGTATCA‑TGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGt < 7:112734/135‑1 (MQ=255) cccgcgCCCGCACATGCGGGCCTAAATTTACCGCGCCCTGCGTTTGCCGGGCG‑CTTTCATTTGCGCAATCGGCGCACTGCCGTATCA‑TGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCa < 6:368638/133‑1 (MQ=255) cccgccCCCGCACTGGGGGGCCTATAATTCCCGCCCCGGGCCTTTGCGGGGCG‑CTTTCATTTGCGCAAGCGGCGCACTGCCGTATCA‑TGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCa < 1:31159/133‑1 (MQ=255) aCGCGCCCGAACTGGGGGGCCTATAATTTCCGCGCCCGGCGTTGGCTGGGGG‑ATTTCATTTGCGCAAGCGGCGCACTGCCTTATCA‑TGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAg < 4:57584/135‑1 (MQ=255) gCCTCTATTTGCCCCGCCCGGCGTTTGCTGGGCG‑CTTTCATTTGCGCAAGCGGCGCACTGCCGTATCA‑TGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGc < 2:121649/135‑1 (MQ=255) gCCTCTAATTTCAGCGCCCTGCCTTTGCTGGGCG‑CTTTCATTTGCGCAATCGGCGCACTGCCGTATCA‑TGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGc < 5:362425/135‑1 (MQ=255) | GTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCG‑CTTTCATTTGCGCAATCGGCGCACTGCCGTATCA‑TGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGC > ACB122/4287687‑4287929 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |