Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,363,536 C→T 6.5% intergenic (‑507/‑144) CJ019_04109 ← / → CJ019_04110 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,363,5360CT 6.5% 198.2 / 3.3 62intergenic (‑507/‑144)CJ019_04109/CJ019_04110hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (17/40);  new base T (2/2);  total (20/42)
Fisher's exact test for biased strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

CTGATTTTCGGGCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCGAGAAGATGGCACCGAGCCATCAAACCATTTAATCTGCACGCTTCGATGCGAATGTG  >  ACB122/4363402‑4363670
                                                                                                                                      |                                                                                                                                      
cTGATTTTCGGGCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGAc                                                                                                                                        <  3:350334/135‑1 (MQ=255)
     tttCGGGCCGTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAg                                                                                                                                   <  5:112443/135‑1 (MQ=255)
          ggCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCTGAGTCTTCATCTCATAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              <  8:339707/135‑1 (MQ=255)
          ggCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              <  1:151624/135‑1 (MQ=255)
          ggCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              <  7:355702/135‑1 (MQ=255)
          ggCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              <  7:342964/135‑1 (MQ=255)
          ggCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              <  5:128485/135‑1 (MQ=255)
          ggCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              <  4:358067/135‑1 (MQ=255)
          ggCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              <  3:168289/135‑1 (MQ=255)
          ggCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              <  2:6816/135‑1 (MQ=255)
           gCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCg                                                                                                                             <  7:147172/135‑1 (MQ=255)
           gCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCg                                                                                                                             <  5:350329/135‑1 (MQ=255)
           gCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCg                                                                                                                             <  3:2505/135‑1 (MQ=255)
            ccTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGa                                                                                                                            <  4:298414/135‑1 (MQ=255)
            ccTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGa                                                                                                                            <  4:294667/135‑1 (MQ=255)
                   tGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAggg                                                                                                                      <  1:43471/134‑1 (MQ=255)
                     cccACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCTCTAAAAGAGACTGAAGCTTTCGAGCAGGGtt                                                                                                                    <  3:142338/134‑1 (MQ=255)
                     cccACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGtt                                                                                                                    <  3:214479/134‑1 (MQ=255)
                     cccACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGtt                                                                                                                    <  6:367368/134‑1 (MQ=255)
                       cACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTtata                                                                                                                 <  4:81397/135‑1 (MQ=255)
                              cAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCt                                                                                                            <  8:256827/133‑1 (MQ=255)
                              cAGTACTGCAGCAGTAGCGTGAAAAGGGAGGCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCtt                                                                                                           <  7:365967/134‑1 (MQ=255)
                               aGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTca                                                                                                         <  2:17511/135‑1 (MQ=255)
                               aGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTc                                                                                                          <  3:354013/134‑1 (MQ=255)
                               aGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTc                                                                                                          <  4:323821/134‑1 (MQ=255)
                               aGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTc                                                                                                          <  1:298289/134‑1 (MQ=255)
                                    tGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACAccc                                                                                                    <  7:229409/135‑1 (MQ=255)
                                       agcagTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCt                                                                                                  >  7:260348/1‑134 (MQ=255)
                                         cagTAGCGTGAAAATGGATTCTCGGGCTGCGCCACATCCGGCCTGAGCACCTCACTCGGAGTCGTGAGCTCCTAATTGATACACAAAAAAAAAAAGAAGCATTAGCGAAAGGTTTTACCACATCACACaccaccc                                                                                               >  2:298289/1‑134 (MQ=255)
                                         cagTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTGCCCACCAAGGGCATGCACATAAATAGACAGCAGCTGTGGCACCGGGTTGTACCCCGAAACAccccac                                                                                                 >  8:136593/1‑131 (MQ=255)
                                         cagTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGGTATACCCCTTCACACCCCTCCa                                                                                               >  8:112411/1‑135 (MQ=255)
                                          agTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCAt                                                                                              <  4:329866/135‑1 (MQ=255)
                                            tAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTGTTGCACTAAAAAAGACTGAAGCGTTCGCGCAGGGTTTTTCCCCGTCACACCCCCCCCTGc                                                                                            >  2:114444/1‑135 (MQ=255)
                                              gCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCCGCGCCTAGGTGTTCCACGAAAATACACAGAAGCTTTAGCGCCGGGTTCTTCCCCTGCCCCCCCCGCCAAGc                                                                                            >  7:157621/1‑133 (MQ=255)
                                              gCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTGTTGCACTAAAAGAGACGGAAGCTTTCGAGCAGGGGTATTACCCGTCACACCCCACCAAGc                                                                                            >  5:184261/1‑133 (MQ=255)
                                                 tGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACGAAAATAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACCCCCCGCCATGCTTatg                                                                                       >  8:291048/1‑133 (MQ=255)
                                                 tGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCGAGGTCTTACACAAAAAGAGACAGAAGCTTTCGAGCAGGGTTATTCCCCATCACACCCCTCCATGCTTAGc                                                                                       >  8:38903/1‑135 (MQ=255)
                                                   aaaaTGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCCGCTCCTAGGGCTTCCACGAAAAGACACTGAAGCGTTCGAGCCCGGGTATACCCCGTCACACCCCACCAAGCGTAGccc                                                                                     >  1:100653/1‑134 (MQ=255)
                                                    aaaTGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              >  7:315300/1‑93 (MQ=255)
                                                    aaaTGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              <  8:315300/93‑1 (MQ=255)
                                                    aaaTGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAg                                                                                    <  5:307498/135‑1 (MQ=255)
                                                    aaaTGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAg                                                                                    <  5:325387/135‑1 (MQ=255)
                                                    aaaTGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAg                                                                                    <  3:268471/135‑1 (MQ=255)
                                                          aTTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCAtct                                                                               <  7:259270/134‑1 (MQ=255)
                                                                            aGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTAtt                                                            <  2:309549/135‑1 (MQ=255)
                                                                              ccGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCg                                                          <  1:163956/135‑1 (MQ=255)
                                                                                       gCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTACACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCGAGAAGAt                                                   <  4:167492/133‑1 (MQ=255)
                                                                                         aCCTCACTCGGAGTCGTGAGCGGCCAGGTGGTCCACAAAAAAAGACAGAAGCTTTAGAGCCGGGGTATAACCCCTAACACACCTCCATGCGTTGCCCGCAACACCATAAAAAAATATCTATAAGAGAAGATGGCa                                               >  2:163956/1‑135 (MQ=255)
                                                                                         aCCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              >  2:217747/1‑56 (MQ=255)
                                                                                         aCCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTc                                                                                                                              <  1:217747/56‑1 (MQ=255)
                                                                                                   gAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCGAGAAGATGGCACCGAGCCAt                                      <  5:254334/134‑1 (MQ=255)
                                                                                                                   ggTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCtt                                                                                          <  3:272230/66‑1 (MQ=255)
                                                                                                                   ggTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCtt                                                                                          >  4:272230/1‑66 (MQ=255)
                                                                                                                   ggTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCGAGAAGATGGCACCGAGCCATCAAACAATTTAATCTGc                     >  4:298512/1‑135 (MQ=255)
                                                                                                                     tCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCGAGAAGATGGCACCGAGCCATCAAACCATTTAATCTGCAc                   >  3:87843/1‑135 (MQ=255)
                                                                                                                           aCTAAAAGAGATTGAAGCTTTCGAGCAGGGTTATACCCCTTc                                                                                                          >  3:19895/1‑42 (MQ=255)
                                                                                                                           aCTAAAAGAGATTGAAGCTTTCGAGCAGGGTTATACCCCTTc                                                                                                          <  4:15178/42‑1 (MQ=255)
                                                                                                                           aCTAAAAGAGATTGAAGCTTTCGAGCAGGGTTATACCCCTTc                                                                                                          >  3:15178/1‑42 (MQ=255)
                                                                                                                           aCTAAAAGAGATTGAAGCTTTCGAGCAGGGTTATACCCCTTc                                                                                                          <  4:19895/42‑1 (MQ=255)
                                                                                                                           aCTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCGAGAAGATGGCACCGAGCCATCAAACCATTTAATCTGCACGCTTCg             >  8:72366/1‑135 (MQ=255)
                                                                                                                           aCTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCGAGAAGATGGCACCGAGCCATCAAACCATTTAATCTGCACGCTTCg             <  8:260348/135‑1 (MQ=255)
                                                                                                                           aCTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCGAGAAGATGGCACCGAGCCATCAAACCATTTAATCTGCACGCTTCg             <  2:100653/135‑1 (MQ=255)
                                                                                                                                aaGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCGAGAAGATGGCACCGAGCCATCAAACCATTTAATCTGCACGCTTCGATGc         >  4:110176/1‑134 (MQ=255)
                                                                                                                                      cTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCGAGAAGATGGCACCGAGCCATCAAACCATTTAATCTGCACGCTTCGATGCGAAtgtg  <  1:282245/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CTGATTTTCGGGCCTTTTTTGCCCACCTTCCAGTACTGCAGCAGTAGCGTGAAAATGGATTCTCGGGCTGCGCCACAGCCGGCCTGAGCACCTCACTCGGAGTCGTCAGCTCCTAGGTCTTCCACTAAAAGAGACTGAAGCTTTCGAGCAGGGTTATACCCCTTCACACCCCTCCATGCTTAGCCAGCATCTCCTTAAATCAGTATCTATTCGAGAAGATGGCACCGAGCCATCAAACCATTTAATCTGCACGCTTCGATGCGAATGTG  >  ACB122/4363402‑4363670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑