Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,500,206 A→C 36.3% R63R (CGT→CGG)  cysG_1 ← Siroheme synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,500,2060AC36.3% 23.0 / 2.4 31R63R (CGT→CGG) cysG_1Siroheme synthase
Reads supporting (aligned to +/- strand):  ref base A (13/6);  new base C (10/2);  total (23/8)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGGCGCATCCACCACATTGACCGGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCAAGGCGATCTCAC  >  ACB122/4500075‑4500333
                                                                                                                                   |                                                                                                                               
gCCGGCGCATCCACCACATTGACCGGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCACCCGACCAGGTCGGCTGCCTGATAGCCACGc                                                                                                                              >  8:190758/1‑135 (MQ=255)
 ccGGCGCATCCACCACATTGACCGGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGCTCACCAGCCGGCAACCGACCAGGTCGGCGGCCTGATAGCCACGcc                                                                                                                             >  1:307431/1‑134 (MQ=255)
  cGGCGCATCCACCACATTGACCGGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCa                                                                                                                             >  6:156286/1‑134 (MQ=255)
  cGGCGCATCCACCACATTGACCGGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCCGCTGCCTGATAGCCCCGccc                                                                                                                            >  7:299803/1‑133 (MQ=255)
  cGGCGCATCCACCACATTGACCGGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCACCCCGCCAACCCCCAGCTCGGCAGCCTGATATCCACGccc                                                                                                                            >  1:106876/1‑133 (MQ=255)
   ggCGCATCCACCACATTGACCGGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCCCGCAcc                                                                                                                           >  5:164196/1‑135 (MQ=255)
     cgcATCCACCACATTGACCGGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCACCAg                                                                                                                         <  3:241532/135‑1 (MQ=255)
       cATCCACCACATTGACCGGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCACCAGCa                                                                                                                       <  6:153278/135‑1 (MQ=255)
            accacATTGACCGGCAGGCTAAGGGCCTGCTCATCGGCCGACACCAGGGCAATCAGCCCAGGCTCGTAAGTCGCCGCCATCACCAGCCCGCAACCGACCCGCTCGGCAGCCCGATAGCCACCCCCCAGCAccaaa                                                                                                                  >  3:254212/1‑132 (MQ=255)
                caTTGACCGGCATGCTAAGGGCCTGCTCAGGGGCCGACACCTGGGCATACAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCTCCAGCACCTCACCGc                                                                                                              <  7:31822/135‑1 (MQ=255)
                          cAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGAATTCAGCCCAGCGTCGTCAGTCGCCGCGATCACAAGCCGTCAACCCAACAGTTCGGCTGCCTGATAGCCACGCACCACCCCCACACCCACCCCATCCCg                                                                                                    >  8:31822/1‑135 (MQ=255)
                           aGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGAACACCAGCCGGCAACCCACCAGGGCGGCTGCCTGGTTGCCACGCACCAGCCCCTCACCGCCCCCGTccccg                                                                                                   >  5:78702/1‑133 (MQ=255)
                             gCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGCCCAGGTCGGCTGCCGGATAGCCCCGCACCAGCCCCTCACCGCCCCCGTCCCGGGc                                                                                                 >  5:340940/1‑135 (MQ=255)
                                  gggCCTGCGCATCGGCCGCCACCTGGGCATTCAGCCCAGGGTCGGCAGTCGCCGCGATCACCAGCCGGCCCCCGCCCCGGGCGGCGGGCGGGGAGCCCCGCCCCCGCCCCGCCCCCCCACCCTCCCGGGcccccg                                                                                            >  7:377738/1‑131 (MQ=255)
                                          gcATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCCCCAGCCGGCAACCGACCAGGTCGGCTGCCTGCTAGCCCCGCCCCAGCCCCTCCCCGCCCCCGTCCCGGGCCACCCCCGCCa                                                                                      >  4:287228/1‑133 (MQ=255)
                                          gcATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCCCCCGCCCAGGTCGGCTGCCTGATAGCCCCGCCCCCGCCCCGCACCGCCCCCGGCCCCGGCCCACGCGGccccc                                                                                    >  3:245975/1‑132 (MQ=255)
                                                 ccGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATGACCAGCCGGCCACCGGCCAGGTCGGCTGGCTGATAGCCCCGCCCCAGCACCTCCCCGCCCCCGTCCCGGGCCAACGCGGCCAACAGGCCgg                                                                             >  3:48550/1‑134 (MQ=255)
                                                 ccGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACg                                                                                                                               >  8:174637/1‑85 (MQ=255)
                                                 ccGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACg                                                                                                                               <  7:174637/85‑1 (MQ=255)
                                                              aTTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGCCCAGGTCGGCTGCCTGATAGCCACGCACCAGCCCCGCCCCGCCCCCGTCCCGGGCCAACGCGGCCCACTGGCCGTCGCCGTCCGgcgc                                                                >  8:20021/1‑135 (MQ=255)
                                                              aTTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCCCGCACCAGCACCTCACCGCCACCGTCCCGGGCCCACGCGGCCAACTGGCCGTCGCCGTCCGgcgc                                                                >  1:77868/1‑135 (MQ=255)
                                                               ttCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGcc                                                               <  8:55130/135‑1 (MQ=255)
                                                                  aGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCAGATAGACACGCACCAGCCCCTCACCCCCCCCTTCCCCGGCCAACCCGGCCAACAGGCCCTCGACGTACGGCGccacc                                                            >  4:218162/1‑135 (MQ=255)
                                                                   gCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCCACCGCCCAGGTCGGCTGCCGGATAGCCCCGCACCCGCCCCTCCCCGCCCCCGTCCCGGGCCCACGCGGCCAACAGGCCGGCGCCGTCCCGCCccaccc                                                           >  2:341329/1‑134 (MQ=255)
                                                                     ccAGGGTCGTCAGTAGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCCCGCCCCAGCACCACCCCGCCCCCTTCCCGGGCCCACGCGGCCAACTGGCCGTCGCCCTCCGGCGCCACCCcg                                                         >  1:57356/1‑135 (MQ=255)
                                                                                 gTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGGCGACGTCCGGCGCCACCACCCGCACCAcgccc                                             >  2:235716/1‑134 (MQ=255)
                                                                                                 gCCGGCAACCGACCAGGTCGGCGGCCTGATCGCCCCGCACCAGCACCGCACCCCCACATACCCGGCCCACCGCGGCAAACTGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCcagca                             <  8:331433/135‑1 (MQ=255)
                                                                                                          cGACCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCACCt                                                                                                         >  4:303162/1‑50 (MQ=255)
                                                                                                          cGACCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCACCt                                                                                                         <  3:303162/50‑1 (MQ=255)
                                                                                                                         ccTGATAGCCACGCACCAGCACCTCACCGCCACCTTCCCGGGCCAACCCGGCCAACTGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCCGCAACGGCCAGCAGGCGCGCCTTGCGCCAGCCGctct     >  1:337531/1‑135 (MQ=255)
                                                                                                                            gATAGCCCCGAACCAGCACCTCAGGGCAACCTACCGGGACCAACGCGGCAAAGTGGCGGTCGACGTCCGGCGCCACCACGCGCTGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGGGCAAGGCGATCTCAc  <  4:254212/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                               
GCCGGCGCATCCACCACATTGACCGGCAGGCTAAGGGCCTGCGCATCGGCCGACACCTGGGCATTCAGCCCAGGGTCGTCAGTCGCCGCGATCACCAGCCGGCAACCGACCAGGTCGGCTGCCTGATAGCCACGCACCAGCACCTCACCGCCACCTTCCCGGGCCAACGCGGCCAACTGGCCGTCGACGTCCGGCGCCACCACGCGCAGCACGCCGCCGGCATCGGCCAGCAGGCGCGCCTTGCGCAAGGCGATCTCAC  >  ACB122/4500075‑4500333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑