Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,747,255 T→G 30.2% S294A (TCG→GCG)  macB → Macrolide export ATP‑binding/permease proteinMacB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,747,2550TG30.2% 43.1 / 7.1 46S294A (TCG→GCG) macBMacrolide export ATP‑binding/permease proteinMacB
Reads supporting (aligned to +/- strand):  ref base T (16/15);  new base G (9/6);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGACCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGC  >  ACB122/4747121‑4747381
                                                                                                                                      |                                                                                                                               
tGGACCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGt                                                                                                                                 <  1:71089/135‑1 (MQ=255)
gggACCGTGGTGCAACCCAGCACGGGGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGt                                                                                                                                 <  1:183898/134‑1 (MQ=255)
 ggACCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGt                                                                                                                                 >  1:264827/1‑134 (MQ=255)
 ggACCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGt                                                                                                                                 <  2:264827/134‑1 (MQ=255)
   aCCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCgg                                                                                                                              <  8:324596/135‑1 (MQ=255)
     cGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGTCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggtg                                                                                                                            <  4:335144/135‑1 (MQ=255)
     cGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggtg                                                                                                                            <  3:355662/135‑1 (MQ=255)
         gtgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATAGCTGCAGGCGGCCTGGCGAGTGATGTGGAGCAACCGCTTCCGCACGGCACTGACACTGCTCGGAATCATCATCGGAGTCGCGGCgggggtgg                                                                                                                        >  8:196921/1‑135 (MQ=255)
          tgaaaCACAGCACGGTCCCTGGAAAGTTGAAATCCTCGAATAGCTGCAGACGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAAACATCATCGGAGTCGCGTCggtggtgg                                                                                                                        >  4:76045/4‑134 (MQ=255)
          tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGTGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggtggtggt                                                                                                                       >  8:229972/1‑135 (MQ=255)
          tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGACTCATCATCGGAGTCGCGGCgggggtggt                                                                                                                       >  4:282131/1‑135 (MQ=255)
           gCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGTGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGgtggtggtg                                                                                                                      <  7:229972/135‑1 (MQ=255)
                  aGCCCGGTGCCTGGAAAGGTGAACTTCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTAGGCATCATCATCTGAGTCGCGGCTGTGGTTGTGTATGTTg                                                                                                               >  6:215699/1‑135 (MQ=255)
                  aGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGGGGTGGGGTGG‑AAGTTgg                                                                                                              >  5:271440/1‑135 (MQ=255)
                       ggTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGGTGTc                                                                                                         >  5:275450/1‑135 (MQ=255)
                       ggTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGGGGGGGTG‑ATGGTGGgtgtg                                                                                                         >  1:258088/1‑134 (MQ=255)
                            cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGt                                                                                                      >  4:201562/1‑133 (MQ=255)
                            cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGGGGTGGGG‑GTGTTTGCTGTGGGt                                                                                                      >  5:16017/1‑133 (MQ=255)
                                 aaGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCACCCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGgca                                                                                               <  1:217883/135‑1 (MQ=255)
                                 aaGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGgca                                                                                               >  4:372021/1‑135 (MQ=255)
                                      gAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGTGTGGCGGTGGAGGCAGCaaa                                                                                          >  1:243931/1‑135 (MQ=255)
                                        aCTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACg                                                                                        <  4:190510/135‑1 (MQ=255)
                                        aCTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTACCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGGGGTG‑ATGTTGGGAGTCGGTGAAGGGAGCAAACg                                                                                        >  3:307207/1‑135 (MQ=255)
                                            cTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAg                                                                                    >  8:250/1‑135 (MQ=255)
                                               gAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACCCCAGGTa                                                                                 >  3:189533/1‑135 (MQ=255)
                                                   cGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGTAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATgg                                                                             <  8:16811/135‑1 (MQ=255)
                                                                         gATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGGCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTc                                                       <  6:114879/135‑1 (MQ=255)
                                                                         gATGTGGAGCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTc                                                       <  8:132594/135‑1 (MQ=255)
                                                                           tggcgATCAACCGCTTCCGCACGGCACTGACCCTGCGCGGTATCATCATCGGAGTCGCGGCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCa                                                     <  7:59434/131‑1 (MQ=255)
                                                                               gacaacccGCTGCCGCACGGCACGGACCCGGCTCGGAATCATCATCGGAGTCGCGGCGGTGGGGGTG‑ATGTTGGCTGGCGGTCAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACAt                                                 <  4:2905/129‑1 (MQ=255)
                                                                                aTCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGAAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATc                                                <  7:364754/135‑1 (MQ=255)
                                                                                   aaCCGCTTCCGCACGCCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCCGTGCGGGTG‑AAGTTGGCTGTCGGTGAAGGCACCAAACGCCAGGTTATGGCACACATGGCCGCCTTCGGATCCAACATTCt                                              >  8:59434/1‑134 (MQ=255)
                                                                                            cGCACGGCACTGACCCTGCTCGGAATCATCCTCGGAGTCGCGGCGGTGGTGGTG‑ATGTTGGCTGGCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAAt                                    <  6:315705/135‑1 (MQ=255)
                                                                                            cGCACGGCACTGACCCTGCGCGGAATCATCATCGGAGGCGCGTCGGTGGGGGTG‑ATGTTGGCTGGCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAAt                                    <  7:294367/135‑1 (MQ=255)
                                                                                             ggaCGTCACTGACCCTGATATGAATAATCATTCGAGTCGAGTGGGTGGTGGTG‑ATGTTGGCTGTGGGAGAAGGCAGAAAACGCCATGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAAt                                    <  8:150172/132‑1 (MQ=255)
                                                                                             gCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAAt                                    <  7:250/134‑1 (MQ=255)
                                                                                             gCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGGGGTG‑ATGTTGGCGGTCGGTGAAGGCAGCAAAAGCCAGGGAATGGCCCAGATGGCCGCCTTCTGCTCCAACATCCTATACCTCaaag                                   >  2:300534/1‑133 (MQ=255)
                                                                                              cACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTTAAGGACGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATgg                                  <  6:271440/135‑1 (MQ=255)
                                                                                              cACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGGGGTT‑ATGTTGGCTGGCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATgg                                  <  2:283743/135‑1 (MQ=255)
                                                                                              cACGGCACTGACCCTGCTCGGAATCATCAGCGGAGTCGCGTCGGTGGTGGTT‑ATGTGGGCGGGCGGGGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATgg                                  <  3:282131/135‑1 (MQ=255)
                                                                                                      tGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGGGGGGGTG‑GAGTTGGCTGGGGGTGCACGCACCAAACGCCACGGAATTGGCCAGATTGCCGCCGTCGGCTCCCACCACCAATACCACAAGAGCCGccccc                          >  2:37714/1‑135 (MQ=255)
                                                                                                         cccTGCTCGGAATCATCATCGGAGTCGCGTCGGGGGGGGTG‑ATGTTTGGTGTGGGTGGAGGCAGCAAACGCCAGGTTATGGCACAGAATGCCGCCGTCGGCTGCAACATACAATACCTCCATGGCAGcccccccc                       >  8:182158/1‑133 (MQ=255)
                                                                                                                cGGAATCATCATCGGAGTCGCGTGGGTGGTGGTG‑TTGTTTGCTGTGGGTGAAGGCAGCCAACGCCAGGTATTGGCACAGCTGGCCGCCTTCGGCGCCACCATCCTATACCGCAATGGCAGCCCCCCCACCCAgcg                >  4:273854/1‑135 (MQ=255)
                                                                                                                cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTT‑ATGTTGGCGGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTAAAAGGCAGCCCCCCCACCCAgcg                >  5:115092/1‑135 (MQ=255)
                                                                                                                cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTgcg                >  3:314748/1‑135 (MQ=255)
                                                                                                                cGGAATCATCATCGGAGTCGCGTCGGGGGTGGGG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGGAATGGCACAGATTGCCCCCGTCGGCGCCAACATCCTATACCGCAAAGGCAGCCCCCCCCCCCAgcg                >  2:273136/1‑135 (MQ=255)
                                                                                                                cGGAATCATCATCGGAGTCGCGGAGGTGGTGGTG‑ATGTTGGGTGTAGGTGAAGGCAGCAAACGCCACGTAATTTCACAGATGGCCGCCTGCGGCACCAACCTCCTTTAACACAATTGCAGCCCCCCGCCCCTgcg                >  4:260298/1‑135 (MQ=255)
                                                                                                                              gcggggcggcgGTGGGGGTG‑ATGTGGGCTGGCGGTGAAGGCCGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgc  <  6:16017/130‑1 (MQ=255)
                                                                                                                              gAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgc  <  4:348483/135‑1 (MQ=255)
                                                                                                                              gAGTCGCGGCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgc  <  3:201562/135‑1 (MQ=255)
                                                                                                                                gTCGCGTCGGTGGGGGTG‑ATGTTTGCTGTCGGGGAAGGCCGCAAACGCCAGGTTATGGCACAGATGGCCGCCTTCGGCTCCAACATACGATACCTCAATGGCCGCCCCCCGACCCGGCGCGACCCGGCGGGgc  >  8:298607/1‑133 (MQ=255)
                                                                                                                                      |                                                                                                                               
TGGACCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTG‑ATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGC  >  ACB122/4747121‑4747381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑