Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,840,685 G→T 9.3% L490L (CTG→CTT)  copA_3 → putative copper‑importing P‑type ATPase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,840,6850GT 9.3% 165.9 / 6.8 54L490L (CTG→CTT) copA_3putative copper‑importing P‑type ATPase A
Reads supporting (aligned to +/- strand):  ref base G (37/12);  new base T (2/3);  total (39/15)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

GTGGCACCTGGACCCGACACGGGCGTTCTGGATCGTACTGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAGCCAGGCCGCCGACC  >  ACB122/4840553‑4840818
                                                                                                                                    |                                                                                                                                     
gtggCACCTGGACCCGACACGGGCGTTCTGGATCGTACTGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCctgct                                                                                                                                     <  8:277178/135‑1 (MQ=255)
gtggCACCTGGACCCGACACGGGCGTTCTGGATCGTACTGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCctgct                                                                                                                                     <  5:166013/135‑1 (MQ=255)
   gCACCTGGACCCGACACGGGCGTTCTGGATCGTACTGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGt                                                                                                                                  >  2:41720/1‑135 (MQ=255)
   gCACCTGGACCCGACACGGGCGTTCTGGATCGTACTGGCCATGCACGTGGCCACCTGCCCTTGTGGCCTGTCTTTGGCTTCGCCACCCGCACTGACCGCCCCCACCGGACCCCTGCACAAGCTTGGCCTGCTgg                                                                                                                                   >  3:27655/1‑134 (MQ=255)
        tGGACCCGACACGGGCGTTCTGGATCGTACTGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGAccc                                                                                                                             >  8:37916/1‑135 (MQ=255)
                  acGGGCGTTCTGGATCGTACTGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGt                                                                                                                   >  8:51411/1‑135 (MQ=255)
                   cGGGCGTTCTGGATCGTACTGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGCCCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTg                                                                                                                  >  2:223067/1‑135 (MQ=255)
                   cGGGCGTTCTGGATCGTACTGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCCCggg                                                                                                                  >  4:63973/1‑133 (MQ=255)
                   cGGGCGTTCTGGATCGTACTGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTg                                                                                                                  >  2:223062/1‑135 (MQ=255)
                   cGGGCGTTCTGGATCGTACTGGCCATGCTCGTGGCCACCTGCCCGTGTGCCCGGTCTTTGGCTACGCCAACCGCACTCACCGCCGCCACCGGAACCCAGCCCAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTg                                                                                                                  >  1:317100/1‑135 (MQ=255)
                               aTCGTACTGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTg                                                                                                      <  5:145347/135‑1 (MQ=255)
                                      tGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTCTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAACCTAGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGaa                                                                                                    >  8:260119/1‑130 (MQ=255)
                                           aTGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGac                                                                                          >  6:66344/1‑135 (MQ=255)
                                           aTGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGATGGTGACCCGCGGCCACGTGCTGGAAGGCCTGACCCAGATCGac                                                                                          >  8:163442/1‑135 (MQ=255)
                                            tGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGaca                                                                                         <  4:27655/135‑1 (MQ=255)
                                                cGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGCCCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACgg                                                                                      >  3:181365/1‑134 (MQ=255)
                                                cGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCCCCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGACCCAGATCGACCCgg                                                                                      >  2:240152/1‑134 (MQ=255)
                                                cGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGATCCTGATCGACACGGt                                                                                     >  1:353459/1‑135 (MQ=255)
                                                cGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGt                                                                                     >  5:21187/1‑135 (MQ=255)
                                                     ccACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACTAGATCGACACGGTGATCt                                                                                >  8:319225/1‑135 (MQ=255)
                                                     ccACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCCGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCt                                                                                >  1:8746/1‑135 (MQ=255)
                                                      cACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCtt                                                                               >  4:28491/1‑135 (MQ=255)
                                                       aCCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTc                                                                              <  3:336562/135‑1 (MQ=255)
                                                          tGCCCTTGTGCCCTGTCTTTGGCTACGCAAACCGCACTGACCGCCGCCACCGGCACCCTGCACACGCATGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCg                                                                             >  1:184829/1‑133 (MQ=255)
                                                           gCCCTTGTGCCCTGTCTTTGGATACGCCAACCGCACTGACCGCCGCCACCGGACCCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCCCGTGCTGGAAGGCCTGACCCAGATCGCCACGGTGATCTTCGACa                                                                          >  7:136570/1‑135 (MQ=255)
                                                             ccTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAg                                                                        <  8:359180/135‑1 (MQ=255)
                                                              cTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGa                                                                       >  6:293942/1‑135 (MQ=255)
                                                               ttGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGAc                                                                      <  4:82033/135‑1 (MQ=255)
                                                                 gtgCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCg                                                                    >  3:192644/1‑135 (MQ=255)
                                                                  tgCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCg                                                                    <  2:233170/134‑1 (MQ=255)
                                                                   gCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGc                                                                  <  8:293120/135‑1 (MQ=255)
                                                                    cccTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGca                                                                 >  5:382705/1‑135 (MQ=255)
                                                                          cTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTTCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGa                                                           >  8:199582/1‑135 (MQ=255)
                                                                          cTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTTCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGa                                                           >  8:194200/1‑135 (MQ=255)
                                                                            ttGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGaca                                                                                         <  2:61024/103‑1 (MQ=255)
                                                                            ttGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGaca                                                                                         >  1:61024/1‑103 (MQ=255)
                                                                            ttGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGAcc                                                         >  3:291186/1‑135 (MQ=255)
                                                                              ggCTACGCGAACCGCACTGACCGCCGCCACCGGAACCCTGCACAATCTTGGCCTTCTGGTGACCCGCTGCCACGTGCTGGAAGGCATGAACCAGATCGACACGGTGATCTTCGACTAGACCGGCACACTGAcc                                                         <  2:64564/133‑1 (MQ=255)
                                                                              ggCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTTCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCg                                                        <  7:199582/134‑1 (MQ=255)
                                                                              ggCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTTCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCg                                                        <  7:194200/134‑1 (MQ=255)
                                                                                      cAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGCCCGAGGgccgcc                                               >  1:380371/1‑135 (MQ=255)
                                                                                      cAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGgccgcc                                               >  8:113442/1‑135 (MQ=255)
                                                                                        aCCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGCTCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTg                                             >  6:218076/1‑135 (MQ=255)
                                                                                            cACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCt                                         >  3:140200/1‑135 (MQ=255)
                                                                                                   cgccgcCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCgca                                   >  3:336519/1‑134 (MQ=255)
                                                                                                             ggAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATccgcccg                        <  2:120544/135‑1 (MQ=255)
                                                                                                                       acaAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGCTCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAg                >  3:192561/1‑133 (MQ=255)
                                                                                                                       acaAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAg                >  8:157353/1‑133 (MQ=255)
                                                                                                                       acaAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAg                <  6:261469/133‑1 (MQ=255)
                                                                                                                       acaAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAg                >  5:261469/1‑133 (MQ=255)
                                                                                                                         aaGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGa                                                                            >  6:328606/1‑71 (MQ=255)
                                                                                                                         aaGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGa                                                                            <  5:328606/71‑1 (MQ=255)
                                                                                                                         aaGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAGCCAg            >  4:198697/1‑135 (MQ=255)
                                                                                                                                 cgtgctgGGCACCGGCGGCCAGGTGCTGGAAGGCCTCAACCAGATCGACACGGTGAGCTTCCACAAGACCGGCACACTGACCTAGGGCCGCCTGATCTTGCGCTGCATCCGCCCGCTCGGAAGCCAGGCCGCCGa    <  8:136570/133‑1 (MQ=255)
                                                                                                                                 ccTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAGCCAGGCCGCCGa    <  8:124818/135‑1 (MQ=255)
                                                                                                                                 ccTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAGCCAGGCCGCCGa    >  6:229983/1‑135 (MQ=255)
                                                                                                                                 ccTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAGCCAGGCCGCCGa    >  5:360014/1‑135 (MQ=255)
                                                                                                                                   tgctgGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAGCCAGGCCGCCGAcc  >  3:254045/1‑135 (MQ=255)
                                                                                                                                   tgctgGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAGCCAGGCCGCCGAcc  >  1:124788/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                     
GTGGCACCTGGACCCGACACGGGCGTTCTGGATCGTACTGGCCATGCTCGTGGCCACCTGCCCTTGTGCCCTGTCTTTGGCTACGCCAACCGCACTGACCGCCGCCACCGGAACCCTGCACAAGCTTGGCCTGCTGGTGACCCGCGGCCACGTGCTGGAAGGCCTGAACCAGATCGACACGGTGATCTTCGACAAGACCGGCACACTGACCGAGGGCCGCCTGACCTTGCGCAGCATCCGCCCGCTCGGCAGCCAGGCCGCCGACC  >  ACB122/4840553‑4840818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑