Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	4,874,709	A→C	28.8%	intergenic (+13/‑174)	CJ019_04568 → / → uacT_1	hypothetical protein/Uric acid transporter UacT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	4,874,709	0	A	C	28.8%	43.2 / 2.9	47	intergenic (+13/‑174)	CJ019_04568/uacT_1	hypothetical protein/Uric acid transporter UacT
Reads supporting (aligned to +/- strand):  ref base A (12/19);  new base C (10/5);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCC  >  ACB122/4874578‑4874843                                                                                                                                    |                                                                                                                                       gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAgg                                                                                                                                      >  4:137725/1‑134 (MQ=255) gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGGGAACTGACAGCCCCTCCCCACGc                                                                                                                                     >  5:106635/1‑135 (MQ=255)      ccggCGCATGGGGTGGCCCGCCTTAGCTTGCCGCTGGCAAACAGCACCCAGATCACCCCCGATCCCCGCACGGTGGGCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc                                                                                                                                  <  8:68088/131‑1 (MQ=255)      ccggCGCAAGCGGTTGCCAGCCATATCATCCCGCTGGCAAACATCACCCAGATGCCCCCCTAAGAGCGAAAGGTGGTGGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc                                                                                                                                  <  7:15266/131‑1 (MQ=255)               ggggTGGCCAGCCAGATCTTGCCGGGGGCCAACATCACCCCGTTGCCCCCCGACGCGCGCCAGCTGGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCaa                                                                                                                       <  2:152920/133‑1 (MQ=255)               gCGGTTGCCAGCCAGTTCATGCCGCTGGGCAAAAACACCCAGATCCCCACCGAAGAGCGCAAGCGCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCaa                                                                                                                       <  1:44505/135‑1 (MQ=255)                 ggTGGCCCGCCAGAGCATGCCGCGGGGAAACATCACCCAGATCCCCCCCGAAGAGGGCATGCGCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAgg                                                                                                                     <  8:97881/135‑1 (MQ=255)                  gTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCACGCCAGGCAACCCCCCCCCCaaag                                                                                                                     >  7:93607/1‑132 (MQ=255)                            agATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCCCCCCCCCAGGGAATCACCTTc                                                                                                          >  4:294656/1‑135 (MQ=255)                              aTCATGCCGCTGGGAAACATCACCCAGATGACCACCGAAGAGCGCACGCGCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTccc                                                                                                        <  5:139806/135‑1 (MQ=255)                               ccagcccGCGGGCCAAAAACACCCAGATCCCCCCCGACGCGCCCACGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTcccc                                                                                                       <  6:363675/130‑1 (MQ=255)                                cATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTGGCAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGCATCACCTTccccg                                                                                                      >  1:86047/1‑134 (MQ=255)                                      gCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCCGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGAATCACCGTCCCCTGCAggc                                                                                                >  7:109326/1‑134 (MQ=255)                                        tGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAGAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGc                                                                                              <  8:119385/135‑1 (MQ=255)                                           gCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCggg                                                                                           >  8:239908/1‑135 (MQ=255)                                                aTCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAATGCGCCCAGGTCAACTGACAGCACCTACCCAGGCAACCCCCCCCCCAAGGGCATCACCTTCCCCAGCAGGAGCCGGCTTGc                                                                                      >  1:76319/1‑135 (MQ=255)                                                  cACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGccc                                                                                    >  6:165181/1‑135 (MQ=255)                                                       aGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCCCCCCCGGCAAATGAAAGCACCAAGCCCCGCAACCCCCCCCCCCAGCGAATCCCCATCCCCGGGCCGAGCCGGCGTGCCcgcgca                                                                               >  1:267904/1‑133 (MQ=255)                                                            accaccTAAGAGCGCACGCTGGTCGGCTACTGGAGCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGTAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGc                                                                          <  8:794/135‑1 (MQ=255)                                                            accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGc                                                                          >  8:284774/1‑135 (MQ=255)                                                            accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATAACCTTCCCCTGCACGCGCGGGCTTGCCCGCGAAGAGGc                                                                          >  3:372740/1‑135 (MQ=255)                                                                 cGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATa                                                                     >  7:246762/1‑135 (MQ=255)                                                                    agagCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGGGCCCAGGTCAACTGACAGCACCTCCCCCGGCAACCCGCCCCCCCACGGAATCACCATCCCCGGCAGGAGCGCGCGTGCCCGCGGAGCGGCCGCTAGcc                                                                  >  4:266676/1‑135 (MQ=255)                                                                      agCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGGGCCCAGGTCAACAGACAGCACCACGCCAGGCAACCCGCCCCCCACGGGATACCCCATCCCCGGCAGGAGCGGGGTTGCCCGCGCAGACGCCCAAAGcccc                                                                >  5:378365/1‑135 (MQ=255)                                                                      agCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATATcccc                                                                >  6:375437/1‑135 (MQ=255)                                                                          accgcgcgtcgGCGAGGGGCCCCCCACCGGCGCCCCGGACAACTCACCCCCCCCCCCCCGCCACCCCCCCCCCCACGGCAACCACCACCCCCTCCCGGCGCGGGCTGGCCCGCTACGAGGCCGATAGCCCCAGcc                                                            <  6:377901/129‑1 (MQ=255)                                                                           aaGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCCACCCGCCCCCCAAGGGAATCACCTTCCCCTGCCGGAGCGGGCTTGCCCGCGCAGCGGCCGCTAGCCCCCGCCt                                                           >  3:79287/1‑135 (MQ=255)                                                                           aaGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGGCAACTGACAGCACCACGCCCGGCCCCCCCCCCCCCCAGGGGATCACCGTCCCCCGCAGGCGCGGGGGTGCCCGCGCACACGCCGCTAGCCCCCGccg                                                           >  4:215891/1‑134 (MQ=255)                                                                                gttcgGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCCCCAGGCAACCCGCCCCCAAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGc                                                      <  6:152851/133‑1 (MQ=255)                                                                                cgtcgGCGACTGGTTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCGGCAGGAGCGGGCTTGCCCGCGCAGAGGCCGCTAGCCCCCGCCGCACGc                                                      >  2:297034/1‑135 (MQ=255)                                                                                cgggggCGACTGCACCCCAAACGCCGCCCTGTTCACCTGACCGCCCCGCCCCCGCCACCCCCCCCCCCCCGTGACTCCCCTGCCCCGGCCGGCGGGGGGTGGCCCGCGACGAGGCCGATAGCCCCAGCCTCAAGc                                                      <  4:213827/131‑1 (MQ=255)                                                                                  tcgGCGACTGGATCGCCAACGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTc                                                    <  8:100311/135‑1 (MQ=255)                                                                                   cgGCGACTGGATCGCCAACGGCGCCCCGGTCAACTCACAGCACCTCGCCAGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCCGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTcc                                                   <  4:79287/135‑1 (MQ=255)                                                                                             agccccAACGGCGCCCCGGTCAACTCACAGCACCGCGCCCGGCAACCCCCCCCCCATGGTAAGCACCTTCCCGTGCCGGAGCGGGCTTCCCGGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                         <  6:106635/131‑1 (MQ=255)                                                                                             acccccAATGGCGCCCAGGGCACCTGACTGCACCCGGCCTGGCACCCCGCCCCCCAAGGAACTCACGTCCCGCTGCAGGAGCGGGCTGGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                         <  6:286829/131‑1 (MQ=255)                                                                                             aTCGCCAAAGGCGCCCAGGTCAACTGACACAACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGTAGCGGGCTGGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                         <  2:76319/135‑1 (MQ=255)                                                                                                 ccAACGGCGCCCAGGTCAACTGACACCACCTCGCCAGGCAACCCCCCCCCCAAGGTAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac                                     <  2:40542/135‑1 (MQ=255)                                                                                                 caaaaGGCGCCCTGGTCAACTGACTGCACCTCGCCAGGCAACCCGCCCCCAAAGGTAATCACATTCCCGTGCTGGAGGGGGCTTGCCGGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac                                     <  6:378365/133‑1 (MQ=255)                                                                                                        cgcCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAAcaagcaa                               >  7:82709/1‑134 (MQ=255)                                                                                                                tCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                      <  8:82709/135‑1 (MQ=255)                                                                                                                tCAACTGACAGAACCACCCCCGGCAACCCGCCCCCCAAGGTAATCACATTCCCCTGCAGTAGCGGGGTTGCCCGCTAAGAGGCCGATTGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                      <  8:178329/135‑1 (MQ=255)                                                                                                                tCAACTCACCCCACCCCGCCACGCACCCCCCCCCCCACGTAAATCCCCTGCCCGTCCCGGCGCGGGCTTCCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                      <  5:375437/135‑1 (MQ=255)                                                                                                                gaaaCTGACCGCACCCCGCCCGCAAACCCCCCCCCCACGGCAATCACCTCCCCCTGCCGGCGCGGGCTGCCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                      <  1:110325/133‑1 (MQ=255)                                                                                                                ccAACTGACCGCCCCTCGCCAGCCACCCCCCCCCACAAGCTAATCACCTCCCCCCGCAGGCGGGGGGTGCCCCGCCAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                      <  5:354718/134‑1 (MQ=255)                                                                                                                acAACTCACACCACCCCCCCAGCCACCCCCCCCCCCACGGAAAACACCTACCCCTCCCGGCGCGGGCTCGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                      <  1:297034/134‑1 (MQ=255)                                                                                                                  aaCTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGCGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgcgc                    >  2:172241/1‑135 (MQ=255)                                                                                                                               gcgcccgccAACCCCCCCCCCAAGGCAACCCCCTGCCCCTCCCGGCGCGGGCTGCCCCGCCAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc       <  3:27544/134‑1 (MQ=255)                                                                                                                                 gCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCt     >  7:301557/1‑135 (MQ=255)                                                                                                                                    aGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCACCAAGCAAGCATCGAGCGCGCAGGCATGAACCCTGcc  >  1:364752/1‑135 (MQ=255)                                                                                                                                    |                                                                                                                                       GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCC  >  ACB122/4874578‑4874843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑