Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,138,3670TG39.5% 43.9 / 12.4 38F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
Reads supporting (aligned to +/- strand):  ref base T (4/19);  new base G (14/1);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 5.01e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTACACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGG  >  ACB122/3138233‑3138499
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aGTACACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGtt                                                                                                                                     <  8:267486/136‑1 (MQ=255)
          aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                           <  8:120287/136‑1 (MQ=255)
          aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTggggg                                                                                                                           >  4:274008/1‑134 (MQ=255)
          aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTggggg                                                                                                                           >  7:6329/1‑134 (MQ=255)
          aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGTTtggg                                                                                                                            >  1:243399/1‑134 (MQ=255)
          aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGTTcgggg                                                                                                                           >  4:223333/1‑134 (MQ=255)
           ttCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                           <  2:292913/135‑1 (MQ=255)
           gtCGGACTGCGTCTGTACGCCATCGGAGACTACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGGTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                           <  2:297114/134‑1 (MQ=255)
               gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTgggcgggggg                                                                                                                      >  5:156785/1‑132 (MQ=255)
               gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTcggcgggcgg                                                                                                                      >  4:54803/1‑132 (MQ=255)
               gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGTTggggggggg                                                                                                                       >  1:249821/1‑132 (MQ=255)
               gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTATGTGCGGCAGCCTGGCCGCGTGTTGTTCGGGGGccg                                                                                                                       >  2:45388/1‑134 (MQ=255)
               gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGAGGTCTGTGCGGCAGCCTGGCCGCGTTGTGTTaggggggccg                                                                                                                      >  8:268152/1‑132 (MQ=255)
                     gTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTc                                                                                                                  <  2:218334/134‑1 (MQ=255)
                       cTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGc                                                                                                               <  2:184713/135‑1 (MQ=255)
                             gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTTTTCGGCGGCCGGGGTCGGCGgccgcc                                                                                                        >  8:76591/1‑135 (MQ=255)
                              ccATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGcac                                                                                                       <  3:274008/136‑1 (MQ=255)
                                  cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGGGGCCGGGTTCAGCGGCAGcccccc                                                                                                    >  2:199901/1‑133 (MQ=255)
                                  cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCAGCGTTTTTTTAGTGCGCCATGTTTAGCAGCAGcaccaca                                                                                                   >  6:336955/1‑135 (MQ=255)
                                       aaTACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTc                                                                                              <  4:243838/136‑1 (MQ=255)
                                       aaTACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGGTCGGCCGCCGGGGTGAGCGGCCGCACCACCgcgtg                                                                                              >  7:173775/1‑132 (MQ=255)
                                                  gcggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGTTCAGCGGCCGCCCCACCCGGTCCGGCGAGAtgc                                                                                   >  4:96936/1‑136 (MQ=255)
                                                   cggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCGGGTTCAGCGGCAGCACCACTGGTTCCGGCGAGAtgct                                                                                  <  7:319492/136‑1 (MQ=255)
                                                      cTAAGGCCGCTGGCCTGTCGGTGCGTGGCTACGTGGTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCTGCAGCACCACCGGTTCCGGCGAGAtgctgc                                                                                <  2:63115/135‑1 (MQ=255)
                                                                   ccTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGGCGGGGTGCGCGGCAGCCCCCCCGGGTGCGGGGGGCTGCTGCGGTGCGgggtggg                                                                   >  1:201641/1‑134 (MQ=255)
                                                                       tCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGAGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCAt                                                                <  5:134355/134‑1 (MQ=255)
                                                                          gTGCGTGGCTACGTGTTCTCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGACGCGGGTTGTTAGGCGGCCAGGTTCAGCGGCCGCTCCACCCGGTGCCGCCAGGAGCTGCGGGCCGAGGGGGCCCTCGCtg                                                           >  8:284231/1‑135 (MQ=255)
                                                                                 gCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTgg                                                    <  3:68526/136‑1 (MQ=255)
                                                                                 gCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTgg                                                    <  6:98665/136‑1 (MQ=255)
                                                                                     cGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTg                                                <  5:36895/136‑1 (MQ=255)
                                                                                     cGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTg                                                <  5:32551/136‑1 (MQ=255)
                                                                                     cGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTg                                                <  5:199731/136‑1 (MQ=255)
                                                                                        gtTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGGTGCGGCGAGATGCTGCTGTCCGTGGTGGCCCTCGCGTTTGTTGGGGTGTtc                                             >  4:213759/1‑136 (MQ=255)
                                                                                        gtTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCGGGTTGCGCGGCCGCCCCACCCGGTGCGGCGAGCTGCTGCTGTGCGTGGTGGGCCTCGGGTTTGTTggggggctg                                             >  8:36712/1‑131 (MQ=255)
                                                                                                        aTCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTg                              <  4:167817/135‑1 (MQ=255)
                                                                                                                    tgCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCAt                   <  3:67087/134‑1 (MQ=255)
                                                                                                                     gCGGCAGCCTGGCCGCGGTTTGTGCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCAt                   <  1:342289/133‑1 (MQ=255)
                                                                                                                        gCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATcggcg              <  2:243399/135‑1 (MQ=255)
                                                                                                                                   gcgTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCCTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTgg  <  5:149529/136‑1 (MQ=255)
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AGTACACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGG  >  ACB122/3138233‑3138499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑