Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB122 | 5,931,671 | 0 | A | C | 32.8% | 92.1 / 10.7 | 62 | T526P (ACC→CCC) | rbbA | Ribosome‑associated ATPase |
Reads supporting (aligned to +/- strand): ref base A (12/29); new base C (20/0); total (32/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.78e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
GACACGCCCGATGCCCTGCAGCACCAGTACCAGGGCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTGGGCGCAGTGTTCATGAT > ACB122/5931536‑5931795 | gACACGCCCGATGCCCTGCAGCACCAGTACCAGGGCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTccaccccca < 6:50243/136‑1 (MQ=255) gATGCCCTGCAGCACCAGTACCAGGGCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCTACCGc > 6:91766/1‑136 (MQ=255) gATGCCCTGCAGCACCAGTACCAGGGCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCACCCTACCg > 2:249361/1‑135 (MQ=255) cccTGCAGCACCAGTACCAGGGCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCCCCCTACCGcgccg > 8:143282/1‑132 (MQ=255) cccTGCAGCACCAGTACCAGGGCGACACGCTGGAAGACGCGGTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACg < 7:31816/136‑1 (MQ=255) cagcaCCAGTACCAGGGCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCggg < 5:260639/136‑1 (MQ=255) ccAGGGCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCTACCGCTACGCCGGGGCTTCAGccag > 6:87245/1‑134 (MQ=255) ccAGGGCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCACCCGACCGCTACGCCCGGGGGTCAGCCTg > 6:281753/1‑136 (MQ=255) ccAGGGCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACACGCGTCCACCCCCCCCCGACCGCTACGCCGGGGCTTCAGccat > 3:17238/1‑134 (MQ=255) ccAGGGCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACAGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCAACCGCTACGCCCGGGCTACAGCCTg > 5:88941/1‑136 (MQ=255) gCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCTACCGCTACGCCGGGGCGTCAGCCTGcgccg > 8:111979/1‑136 (MQ=255) cGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCACCGTGCTGGAACAGGCGTCCACCCCCCCCCCACCGCAACCCCCGGGCTTCAGCCAGcgccgc > 4:208621/1‑136 (MQ=255) cGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGcgccgc < 8:66454/136‑1 (MQ=255) cGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGcgccgc > 8:74994/1‑136 (MQ=255) acacGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCTACCGCTCCGCCGGGGCTTCCGCCTGCGCCGCct > 3:193835/1‑136 (MQ=255) acacGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCGACCGCTACGCCGGGGCGTCAGCCTGCgccgccg > 3:295961/1‑135 (MQ=255) acGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCGACCGCGACCCCGGGGCCTCCGCCAGCGCCGCcggc > 5:314712/1‑136 (MQ=255) cGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCACCCTACCGCTACGCCGGGGCGTGCGCCTGCGCCGCctgct > 3:235658/1‑136 (MQ=255) cGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACCGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCCCCCAACCCCTACGCCGGGGCGTCAGCCGGCGCCCCctgc > 2:186858/1‑135 (MQ=255) gCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCctgctg < 3:306848/136‑1 (MQ=255) gAAGCCGCGTGCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGc < 5:34461/134‑1 (MQ=255) gAAGACGCGGTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGc < 8:257811/134‑1 (MQ=255) aaGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCTACCGCGACGCCCGGGCTTCCGCCTGCGCCGCCTGCTGGCCGt > 6:215340/1‑136 (MQ=255) aaGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCGACCCCGACGCCGGGGGGTCAGCCGGCGCCGCCGGCGGGccgg > 6:153357/1‑133 (MQ=255) aaGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCGTAAGCCAGCGCCGCCTGCTGGCAGt > 6:97852/1‑136 (MQ=255) aaGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGAGTGCCCCGCCACCCTACCGCAACCCCGGGGGTTCAGCCTGACCCGCCTGCTGGCAGt > 8:267938/1‑136 (MQ=255) ttCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGccgtcc < 7:30402/136‑1 (MQ=255) gcggccGCTGCCTGGAACCGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGGCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGccgtcc < 2:176409/131‑1 (MQ=255) cGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAgg < 6:95293/136‑1 (MQ=255) ccAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTgcgc < 6:254739/136‑1 (MQ=255) cAGGTACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCCCCCTACCGCTACGCCGGGGCGTCCGCCTGCGCCGCCTGCTGGCAGTGGCCGGCCGGGAGGGCAAGGAGTTGCTGcgcg > 7:185977/1‑136 (MQ=255) cAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCGACCGCGACGCCGGGGCTTCAGCCTGCGCCGCCGGCGGGCAGTGGCCGTCCGGGCGGGCAAGGAGTTGCTgcgc > 1:209320/1‑135 (MQ=255) cAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCAACCCCTACGCCCGGGCTTCCGCCTGCGCCCCCTGCTGGCAGTGGCCGTGCGGGAGGGCAAGGACTTGCTGcgcg > 4:150164/1‑136 (MQ=255) cAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCATCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGcgcg > 6:30646/1‑136 (MQ=255) cAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCCCCCTACCGCTACGCCGGGGCTTCAGCCAGCGCCGCCTGCTGGCCGTGGCCGTCCGTGAGGGCAACGAGTTGCTtcgc > 2:210136/1‑135 (MQ=255) cAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCCACCAACCCCTACGCCGGGGCATAAGCCTGCGCCGCCAGCTTGCAGTGGCCGTCCGGGAGGGCAAGGAGTtgcatcgc > 2:287359/1‑130 (MQ=255) cAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGGGAGGGCAAGGAGTTGCTGcgcg > 3:190228/1‑136 (MQ=255) ggAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCTACCGCTACGCCGGGGCGTCAGCCTGCGCCCCCTGCTGGCAGTGGCCGGCCGGGAGGGCAAGGAGTTGCTGCGCGa > 4:135346/1‑135 (MQ=255) aaCTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCTACCGCAACCCCGGGGCGTCCGCCAGCGCCCCCAGCTGGCAGGGGCCGTGCGTGAGGGCCAGGAGTTGCTGcgcgccca > 8:1877/1‑132 (MQ=255) gcgcCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTgc < 8:112076/136‑1 (MQ=255) gcgcCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTgc < 5:153357/136‑1 (MQ=255) gcgcCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTgc < 3:291367/136‑1 (MQ=255) cgcCAGCCGCCCCCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCCCCCGACCCCAACCCCCGGGCGTCCGCCTTCGCCCCCGGCTGGGCGTGGCCCGACGTGCGGGGCAGGAGTTGCTGCCCCACAAAGTgcg > 4:186955/1‑136 (MQ=255) cgcCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCCCCTGCTGGCAGTGGCCGTCCGTGTGGGGAAGGAGTTGCTTCGCGACAAGGGgcg > 5:230455/1‑136 (MQ=255) gcCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTGCCCCCCCACCCAACCCCATCGCCGGGGCGTCAGCCTGCGCCCCCTGCAGGCAGTGGCCGGCCGGGCGGGCAAGGAGTTTCTTCGCGGCAATGTgcgc > 8:9551/1‑136 (MQ=255) ccAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCCCCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCGGCGGGGAGGGGCCGTCCGGGAGGGCCAGGAGTTGCTGCGCGCCAAGGTGCGcc > 5:82588/1‑136 (MQ=255) gccgccACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTgg < 7:74994/136‑1 (MQ=255) gccACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTCCGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCt < 6:16877/136‑1 (MQ=255) gccACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCt < 3:77337/136‑1 (MQ=255) gccACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCt < 7:111979/136‑1 (MQ=255) aCAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCtt < 5:97852/136‑1 (MQ=255) aGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTAc < 4:261365/136‑1 (MQ=255) aaCGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTgg < 7:240301/136‑1 (MQ=255) aaCGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTgg < 7:267938/136‑1 (MQ=255) aCGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTgg < 1:249361/135‑1 (MQ=255) cGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTGGgc < 5:281753/136‑1 (MQ=255) cGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTGGgc < 6:168967/136‑1 (MQ=255) tGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGTAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTGGgcgc < 5:215340/136‑1 (MQ=255) gCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTGGgcgc < 2:251647/135‑1 (MQ=255) gAACAGGCGTCCACCCCCTCCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTGGGCGCAgtgt < 6:88941/136‑1 (MQ=255) gAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTGGGCGCAgtgt < 3:208621/136‑1 (MQ=255) aaCAGGCGTCCACCCCCACCCTACCGCTACGCCTGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTGGGCGCAGTGtt < 4:17238/136‑1 (MQ=255) cGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTGGGCGCAGTGTTCatgat < 6:314712/136‑1 (MQ=255) | GACACGCCCGATGCCCTGCAGCACCAGTACCAGGGCGACACGCTGGAAGACGCGTTCGTCCGCTGCCTGGAACAGGCCCAGGAACTGGCGCCAGCCGCCACCGACAGCAACGTGCTGGAACAGGCGTCCACCCCCACCCTACCGCTACGCCGGGGCTTCAGCCTGCGCCGCCTGCTGGCAGTGGCCGTCCGTGAGGGCAAGGAGTTGCTGCGCGACAAGGTGCGCCTGGCCTTCGCCTTACTGGGCGCAGTGTTCATGAT > ACB122/5931536‑5931795 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |