Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	1,806,746	G→C	W15C (TGG→TGC)	yeiG →	S‑formylglutathione hydrolase YeiG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,806,746	0	G	C	100.0%	197.3 / NA	58	W15C (TGG→TGC)	yeiG	S‑formylglutathione hydrolase YeiG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (25/33);  total (25/33)

ACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGC  >  ACB122/1806613‑1806872                                                                                                                                      |                                                                                                                               acGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCca                                                                                                                              >  5:297275/1‑136 (MQ=255)          cTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTa                                                                                                                     >  3:297362/1‑136 (MQ=255)             taaGACATCAACAAGGCCTTCGACCTGATGCATGATGGCTAGAGCATCCGTTGCGTGATCCAGTTCTGTTGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTAcc                                                                                                                   <  6:65428/134‑1 (MQ=255)             gAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCg                                                                                                                  <  6:272075/136‑1 (MQ=255)             gAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCg                                                                                                                  <  6:198638/136‑1 (MQ=255)             gAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCg                                                                                                                  <  5:58388/136‑1 (MQ=255)             gAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCg                                                                                                                  <  7:219183/136‑1 (MQ=255)              aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCg                                                                                                                  <  1:14989/135‑1 (MQ=255)              aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCg                                                                                                                  >  1:291441/1‑135 (MQ=255)               aGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCgg                                                                                                                 <  2:319025/135‑1 (MQ=255)                      aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGcatcatcc                                                                                                         >  5:104869/1‑134 (MQ=255)                      aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCAtt                                                                                                          <  4:103811/135‑1 (MQ=255)                      aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCAtt                                                                                                          <  7:65432/135‑1 (MQ=255)                      aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTc                                                                                                         >  7:181832/1‑136 (MQ=255)                      aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCAGCATCAtt                                                                                                          <  3:259706/135‑1 (MQ=255)                           ggCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAgg                                                                                                    >  4:212196/1‑136 (MQ=255)                           ggCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAgg                                                                                                    >  4:216258/1‑136 (MQ=255)                               ttCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                                >  7:34444/1‑136 (MQ=255)                                 cGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                                <  8:155789/134‑1 (MQ=255)                                    ccTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGtt                                                                                           >  6:144631/1‑136 (MQ=255)                                     cTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGtt                                                                                           <  2:291441/135‑1 (MQ=255)                                        aTGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGa                                                                                       <  4:54645/136‑1 (MQ=255)                                         tGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGa                                                                                       <  2:77571/135‑1 (MQ=255)                                               aaGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgt                                                                                 <  5:25138/135‑1 (MQ=255)                                               aaGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgt                                                                                 <  4:297362/135‑1 (MQ=255)                                                     agagCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCt                                                                          >  8:270391/1‑136 (MQ=255)                                                      gagCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCtg                                                                         >  4:331891/1‑136 (MQ=255)                                                               tAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTgc                                                                >  7:260812/1‑136 (MQ=255)                                                                 gCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTgcc                                                               <  3:216258/135‑1 (MQ=255)                                                                 gCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTgcc                                                               <  3:212196/135‑1 (MQ=255)                                                                         cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGc                                                      >  7:310595/1‑136 (MQ=255)                                                                         cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACAGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTAACTGCCGCCGCAGGc                                                      >  6:106221/1‑136 (MQ=255)                                                                          aCTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGcc                                                     >  8:18437/1‑136 (MQ=255)                                                                              cTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGa                                                   <  5:144631/134‑1 (MQ=255)                                                                              cTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGa                                                   <  3:67477/134‑1 (MQ=255)                                                                                        tCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaa                                         >  3:236546/1‑134 (MQ=255)                                                                                         cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                                >  2:168751/1‑78 (MQ=255)                                                                                         cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                                <  1:168751/78‑1 (MQ=255)                                                                                         cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGc                                                                                         <  7:272280/85‑1 (MQ=255)                                                                                         cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGc                                                                                         >  8:272280/1‑85 (MQ=255)                                                                                         cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAg                                      >  5:222359/1‑136 (MQ=255)                                                                                          aTGAGCCTGGATCACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                                     <  5:265455/136‑1 (MQ=255)                                                                                          aTGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                                     <  8:260812/136‑1 (MQ=255)                                                                                          aTGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                                     <  5:161412/136‑1 (MQ=255)                                                                                           tGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCt                                    >  8:183647/1‑136 (MQ=255)                                                                                               ccTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                                <  4:164178/136‑1 (MQ=255)                                                                                                   gATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGTGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGc                            <  6:39931/136‑1 (MQ=255)                                                                                                    aTAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCt                           <  7:138087/136‑1 (MQ=255)                                                                                                           ctcCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTgg                    >  4:129719/1‑136 (MQ=255)                                                                                                            tcCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                                >  2:225742/1‑59 (MQ=255)                                                                                                            tcCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                                <  1:225742/59‑1 (MQ=255)                                                                                                            tcCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGtt                                                                                           <  2:117588/64‑1 (MQ=255)                                                                                                            tcCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGtt                                                                                           >  1:117588/1‑64 (MQ=255)                                                                                                                 ccAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGc              <  7:18437/136‑1 (MQ=255)                                                                                                                 ccAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTGGCGATATGGTGGTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGc              <  7:237925/136‑1 (MQ=255)                                                                                                                           ttCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCt    >  7:190878/1‑136 (MQ=255)                                                                                                                             cggcggCTGCCACACGCGTTACCGGCATCATTCCAAGGTGCTGGGTGGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCt    <  8:181832/134‑1 (MQ=255)                                                                                                                             cggcggCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCAGGTGCTGTACTGGCTCAGCGGCCTCACCTGc  >  4:204642/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                               ACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGC  >  ACB122/1806613‑1806872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑