| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB122 | 1,836,147 | A→C | V568G (GTG→GGG) | barA ← | Signal transduction histidine‑protein kinaseBarA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,836,147 | 0 | A | C | 95.0% | 51.3 / NA | 21 | V568G (GTG→GGG) | barA | Signal transduction histidine‑protein kinaseBarA |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (17/2); total (19/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGG > ACB122/1836028‑1836277 | ggCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCCGGCCGGCCTGGCTGGCGGCCTGC‑CCCCCTGCAGCAACTgg > 6:159568/1‑136 (MQ=255) cAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCCGGGCGGCCAGGCTGGCGGCCGGCCCCCCCAGCCGCaagcgg > 1:330886/1‑131 (MQ=255) cAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCCGGCCGGCCTGGCTGGCGGCCTGCCCCCCCTGCAGCAACTgg > 2:96637/1‑135 (MQ=255) cAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCCGGCCCGCCAGGCTGGCGGCCTGCCCCCCCAGCAGCAACTGGt > 4:209604/1‑136 (MQ=255) cAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCAGCACCCCCTGCAGCAACAGGt > 6:83614/1‑136 (MQ=255) tCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCCGCCTGGCAGGCGGCCTGACCCCCCAGCAGCAACAGGTCCTAGGAGGCg > 6:139197/1‑135 (MQ=255) tgctgATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCCGGCCGGCCGGGCTGGCGGCCGGCCCCCCCCGCCGCCACTGGGCGGAGGGGGCGCACCGGc > 7:308948/1‑136 (MQ=255) atgatAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCCCCCCCTGGCGCCACTGGTCGTAGGAGGCGCACAGGCTCACg > 5:141907/1‑136 (MQ=255) ggCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCAGGCTGGCGGCCTGCCCCCCCAGCCGCCCCTGGGCGTAGGCGGCGAGCAGGCGAACGCTGCgggc > 5:259634/1‑134 (MQ=255) cccAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACAGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCCCCCCCAGCAGCAACAGGGCGAAGGAGGCGAACAGGCGCACCCTGCcgccgc > 4:74578/1‑136 (MQ=255) ttGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCCGGCCCGCCGGGGTGGCGGCCAGCCCCCCCCGCTGCAAAGGGGCGTTGGCGGCGGACCGGCTCACCCGGCCGCCGCCGTcg > 5:183076/1‑135 (MQ=255) gcgcCAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCCGGCCGGCCTGGCTGGCGGCCGGCCCCCCCTGCAGCAACGGGTCGGAGGAGGCGAACAGGCGCACGCCGAGGCCGCCGGCGTCCAGcgg > 7:66041/1‑134 (MQ=255) gCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGACGGGCAGGCCGGCCTGGCTGGCGGCCTGCCCCCCCAGCCGCAACGGGTCGGAGGAGGCGCACAGGCTCACGCTGAGGCCGCCGTCTTCCAGcgg > 3:159760/1‑134 (MQ=255) aCCGCGAACTCGAAGGGCCGGCCCGCCTGGCTGGCGGCCTGCCCCCCCGGCCGCCACGGGGAGGAGGAGGCGCACACGCGCACCCTGAGGCCGCAGTCTTCCAGCGGGTTCTCCACTGCCACGCGCCCCACTTc > 4:256009/1‑134 (MQ=255) aCCGCGAACTCGAAGGGCAGGCCGGCCTGGGTGGCGGCCTGCCCCCCCTCCAGCAACCTGGCGTAGGAGGCGAACACGCTAACGCGGAGGCCGCAGTCATCCAGCTGGTGCTCCACTGCCTGGCCCGCCAGttac > 2:190502/1‑133 (MQ=255) cgcccggcgGGGCGGGCGGCCCCCCCCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATggcg < 2:23833/132‑1 (MQ=255) cgggcTGGCTGGCGGCCTGCCCCCCCAGCAGCAAATGGTCGTAGGAGGCGCAAACGCTCACCCTGAGGCCGCAGTCTTCCAGCTGGTGCTCAAGTGCCTGGCGCGCCCCTTCGTGGCCATCGACAATGGCGGCAcg > 6:92722/1‑136 (MQ=255) ccTGCCCCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAgg < 7:96237/53‑1 (MQ=255) ccTGCCCCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAgg > 8:96237/1‑53 (MQ=255) ccTGCCCCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATgg > 3:238513/1‑136 (MQ=255) cTGCCCCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACACTGGCGGCACGGCGGCCCAGCAATgg > 1:293620/1‑135 (MQ=255) | GGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGG > ACB122/1836028‑1836277 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |