Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	5,533,095	G→A	R132C (CGC→TGC)	CJ019_05210 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,533,095	0	G	A	98.5%	236.6 / ‑6.3	68	R132C (CGC→TGC)	CJ019_05210	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (27/40);  total (28/40)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGTTGGACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCGGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCATCGT  >  ACB122/5532964‑5533226                                                                                                                                    |                                                                                                                                    cccGTTGGACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATgcagcg                                                                                                                                  >  7:44257/1‑135 (MQ=255) cccGTTGGACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATgcagc                                                                                                                                   >  2:93700/1‑134 (MQ=255)       ggACAGGCGCTGGGCCACCTCGACCACAGCACGCTCCGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGa                                                                                                                            >  1:73326/1‑135 (MQ=255)        gACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAg                                                                                                                          <  8:44257/136‑1 (MQ=255)                cTGGGCCACCTCACCCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCGGCGCAGCGACGCCTCGAt                                                                                                                   >  1:231939/1‑135 (MQ=255)                      cACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCTATGCAGCGCAGCGAAGCCTCGATGccgccg                                                                                                            <  5:139677/136‑1 (MQ=255)                      cACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATgccgcc                                                                                                             <  2:148182/135‑1 (MQ=255)                           cGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCa                                                                                                        >  2:142890/1‑135 (MQ=255)                            gACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                       <  1:218200/135‑1 (MQ=255)                            gACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                       <  4:29810/135‑1 (MQ=255)                            gACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                       <  7:53756/135‑1 (MQ=255)                            gACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGGCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCa                                                                                                        <  6:295310/134‑1 (MQ=255)                            gACCACCGCACGCTCGGCGGTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGGCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                       <  1:139461/135‑1 (MQ=255)                            gACCACCGCACGCGCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCAGCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                       <  1:291870/135‑1 (MQ=255)                                accGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCg                                                                                                  <  6:336/136‑1 (MQ=255)                                 ccGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCCGCGAAGCCTCGATGCCGCCGAGCACGATCgg                                                                                                 >  5:236790/1‑136 (MQ=255)                                 ccGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCg                                                                                                  <  1:93700/135‑1 (MQ=255)                                   gCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGc                                                                                                <  2:42745/135‑1 (MQ=255)                                   gCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGc                                                                                                <  2:204197/135‑1 (MQ=255)                                   gCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGc                                                                                                <  2:153397/135‑1 (MQ=255)                                      cGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGCGCACGATCGGcaca                                                                                             >  2:68422/1‑135 (MQ=255)                                            gCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                      <  3:41604/136‑1 (MQ=255)                                              gTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTa                                                                                     >  2:47284/1‑135 (MQ=255)                                              gTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCGGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAg                                                                                    >  3:6681/1‑136 (MQ=255)                                                tGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAgg                                                                                   <  1:47284/135‑1 (MQ=255)                                                        agcagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCtt                                                                            >  1:209461/1‑134 (MQ=255)                                                          cagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                        <  3:597/136‑1 (MQ=255)                                                          cagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                        <  5:317351/136‑1 (MQ=255)                                                           aggaggTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       <  5:6448/136‑1 (MQ=255)                                                           agGATGTCGGCGCTGGCATCGATCAGGCTCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                        <  1:309490/135‑1 (MQ=255)                                                            ggATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       <  1:206472/135‑1 (MQ=255)                                                             gATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgcgc                                                                     >  6:278082/1‑136 (MQ=255)                                                               tGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGTGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCt                                                                    >  2:132427/1‑135 (MQ=255)                                                               tGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCt                                                                    >  2:132429/1‑135 (MQ=255)                                                                gTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTg                                                                   >  2:229992/1‑135 (MQ=255)                                                                 tCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTgg                                                                  <  7:15956/135‑1 (MQ=255)                                                                     cgcTGGCATCGATCAGGATCGAGTGACGTACCTTGTCCTGCCAGTAGACGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCtata                                                             <  7:229239/136‑1 (MQ=255)                                                                     cgcTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCa                                                                                                                                <  6:173458/69‑1 (MQ=255)                                                                     cgcTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCa                                                                                                                                >  5:173458/1‑69 (MQ=255)                                                                      gcTGGCATCGATCTGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCtata                                                             <  1:31539/135‑1 (MQ=255)                                                                               gATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTg                                                   <  8:123882/136‑1 (MQ=255)                                                                               gATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGGAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCATGCTg                                                   <  7:321127/136‑1 (MQ=255)                                                                                aTCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTg                                                   <  2:209461/135‑1 (MQ=255)                                                                                 tCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCAGATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTg                                                   <  4:161246/134‑1 (MQ=255)                                                                                 tCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGc                                                 <  3:138249/136‑1 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGAt                                                                                                    <  2:273189/73‑1 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGAt                                                                                                    >  1:273189/1‑73 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       <  4:241692/102‑1 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       >  3:241692/1‑102 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGAc                                      >  1:209103/1‑135 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGAc                                      >  1:186687/1‑135 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGAGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACg                                     >  6:286200/1‑136 (MQ=255)                                                                                                  aCCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCGTCGATGCCGCCGAGCACGATCGGCACGGGCTTGTAGGCCTCCTTGCAGCGGTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTg                                 >  2:36339/1‑135 (MQ=255)                                                                                                  aCCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       <  1:238545/97‑1 (MQ=255)                                                                                                  aCCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       >  2:238545/1‑97 (MQ=255)                                                                                                  aCCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGc                                >  7:238567/1‑136 (MQ=255)                                                                                                   cgttGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGcc                               <  4:107928/134‑1 (MQ=255)                                                                                                   ccTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGcc                               >  6:248569/1‑136 (MQ=255)                                                                                                        tCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggc                            <  2:294804/134‑1 (MQ=255)                                                                                                              cAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGccac                    <  5:248569/136‑1 (MQ=255)                                                                                                               agtagtCGTTGTGGGCGTTGCAGCGCAGCGAAGCCGCGATGAGGCCGAGCACGATCGGCACATGCTTGGAGGCCTCCTTGCAGCGCTGGCTTTACACCAGGCTGGCAGGGTCCGGACGGCTGCCGGCCAGGccac                    <  1:132427/135‑1 (MQ=255)                                                                                                               agtagtCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                      <  2:72147/69‑1 (MQ=255)                                                                                                               agtagtCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                      >  1:72147/1‑69 (MQ=255)                                                                                                               agtagtCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTGGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGccac                    <  1:132429/135‑1 (MQ=255)                                                                                                                gtagtCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGccacca                  >  6:248701/1‑136 (MQ=255)                                                                                                                      gTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTgg                                                                  <  7:17312/82‑1 (MQ=255)                                                                                                                      gTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTgg                                                                  >  8:17312/1‑82 (MQ=255)                                                                                                                          tGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAgg        >  7:201008/1‑136 (MQ=255)                                                                                                                          tGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAgg        >  3:62390/1‑136 (MQ=255)                                                                                                                                 tGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCAtcgt  >  2:4324/1‑135 (MQ=255)                                                                                                                                    |                                                                                                                                    CCCGTTGGACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCGGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCATCGT  >  ACB122/5532964‑5533226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑