Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,721,8780AC38.7% 19.9 / 11.9 31G95G (GGT→GGG) CJ019_01617hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (16/3);  new base C (1/11);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 8.83e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGG‑CGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGT  >  ACB122/1721743‑1722012
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gcgGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCAc                                                                                                                                        <  2:218183/137‑1 (MQ=255)
gcgGGGAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCAc                                                                                                                                        <  1:164261/137‑1 (MQ=255)
    gTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCgc                                                                                                                                    >  7:323205/1‑136 (MQ=255)
        aCCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGTACCCAGCGGTCAGCATCTTCTTACCCGTCTTCCTCGACCCAGCCACCCcg‑cgcg                                                                                                                               >  8:162495/1‑137 (MQ=255)
        aCCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGg‑cgcg                                                                                                                               >  8:240427/1‑137 (MQ=255)
               ccTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGG‑Cgcgcagcgca                                                                                                                        >  5:336978/1‑137 (MQ=255)
                 tACAGAAACGACCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATTCGGACCCAGCGGTCAGCATCTTCTACCCCGGCTTCCTAGCCCCAGCCACCCGG‑CGCCCCGCGCAca                                                                                                                      >  7:240180/1‑136 (MQ=255)
                                                     gCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGG‑CGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAg                                                                                  >  7:123214/1‑137 (MQ=255)
                                                     ccgcggTTATTTCCTGTACCCCGGGGGCGATTATCCGCCCCCAGGGGTAATCATCTTATACCCCGTGTTCCTCCACCCCCCCCCCCGG‑GGCCCCGCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAg                                                                                  <  4:28080/133‑1 (MQ=255)
                                                       ccggTGATTTCCTCTACACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTCCCCCGTCTTCCTCGACCCAGCCCCCCGG‑CGCCCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGc                                                                                <  6:132956/135‑1 (MQ=255)
                                                       cAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGG‑CGCGCAGTGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGc                                                                                >  3:123465/1‑137 (MQ=255)
                                                               ttCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCCCCCGG‑CGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCACCGTCAt                                                                        >  1:100752/1‑137 (MQ=255)
                                                                  ctctTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGG‑CGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCgg                                                                     >  2:220352/1‑137 (MQ=255)
                                                                       cACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGg‑c                                                                                                                                  <  8:123438/71‑1 (MQ=255)
                                                                       cACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGg‑c                                                                                                                                  >  7:123438/1‑71 (MQ=255)
                                                                                     tggcgcACCCCGCGGTCACAATGTTCTTCCCCGTCTTCCTCCCCCCCCCCCCCCGG‑CGCGCCGCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCt                                                  <  8:25416/135‑1 (MQ=255)
                                                                                     tggCGGACCCCGGGGTCAGCATCTGCTGCCCGGGCTTCCGCGCCCCACCCCCCGGG‑GGCCCCGCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCt                                                  <  7:156336/135‑1 (MQ=255)
                                                                                             ccAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGG‑CGCGCAGCGCACTTCCACCCCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCGTTCCAGAt                                          >  5:183830/1‑137 (MQ=255)
                                                                                             ccAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGG‑CGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGGGGCTTGGCAGAt                                          >  1:84507/1‑137 (MQ=255)
                                                                                               cgCGGTCAGAATCTTCTACCCCGGCTTCCCCCACCCCCCCCCCCGG‑CGCCCCGCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATAc                                        <  5:309646/136‑1 (MQ=255)
                                                                                               cgCGGACAGCATCTTCTTCCCCGCGTCCCCCCCCCCACCCCCCCGG‑CGCCCCGCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATAc                                        <  4:169586/136‑1 (MQ=255)
                                                                                                ggggTCAGCATCTTCTCCCCCGTCTACCCCCCCCCCCCCCCCGGG‑CGCCCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACa                                       <  5:234215/135‑1 (MQ=255)
                                                                                                  ggTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGG‑CGCGCAGCGCACTTCCCCACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAgg                                     >  8:226652/1‑137 (MQ=255)
                                                                                                  ggTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGG‑CGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAgg                                     >  3:92775/1‑137 (MQ=255)
                                                                                                       tcATCTTCTTCCCCGTCTTCCTCCACCCACCCCCCCGG‑CGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGtt                                <  3:186747/136‑1 (MQ=255)
                                                                                                        aatgttcttcCCCTCCATCCACCCCCCACCCCCCGGG‑CGCGCCGCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTc                               <  4:313151/133‑1 (MQ=255)
                                                                                                        aaTCTTCTCCCCCGTCTACCCCCCCCCCGCCCCCCGG‑CGCGCACCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTc                               <  5:71692/136‑1 (MQ=255)
                                                                                                                     gTCTTCCTCGACCCAGCCACCCGG‑CGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAg                  >  7:205226/1‑137 (MQ=255)
                                                                                                                                cccAGCCACCCGG‑CGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAAc       >  4:266399/1‑137 (MQ=255)
                                                                                                                                 ccAGCCCCCCGGCCGCGCGCCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGTCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTACATTTCCCTACAGCCTTGGGTAAc       <  2:281974/137‑1 (MQ=255)
                                                                                                                                     ccACCCGG‑CGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGt  >  8:27381/1‑137 (MQ=255)
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GCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGG‑CGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGT  >  ACB122/1721743‑1722012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑