Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	5,533,095	G→A	R132C (CGC→TGC)	CJ019_05210 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,533,095	0	G	A	96.1%	256.2 / ‑0.8	77	R132C (CGC→TGC)	CJ019_05210	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (30/44);  total (32/45)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGTTGGACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCGGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCATCGTCG  >  ACB122/5532964‑5533228                                                                                                                                    |                                                                                                                                      cccGTTGGACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCa                                                                                                                                  >  4:179793/1‑137 (MQ=255) cccGTTGGACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGAGGGCGATTCAGCGCa                                                                                                                                  >  1:65892/1‑137 (MQ=255) cccGTTGGACAGGCGCTCTGCCACCTCGACCACCGCACGCTCGGCGGTGCAGAACAGCAGGATGTCTGCGCTGTCATCGATCATGATAGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCa                                                                                                                                  <  1:136394/137‑1 (MQ=255)       ggACAGGCGCTGGGC‑‑CCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGcc                                                                                                                          >  3:30686/1‑137 (MQ=255)            ggCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCg                                                                                                                       <  3:265557/137‑1 (MQ=255)                cTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATgc                                                                                                                   >  6:123157/1‑137 (MQ=255)                     ccACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGGTGCGGCGCAGCGAAGCCTCGATGccgccg                                                                                                              >  1:320847/1‑137 (MQ=255)                     ccACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGccgccg                                                                                                              >  3:32966/1‑137 (MQ=255)                      cACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGa                                                                                                             <  4:32250/137‑1 (MQ=255)                         cTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCa                                                                                                          >  1:306531/1‑137 (MQ=255)                          tCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                         <  1:137102/137‑1 (MQ=255)                          tCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                         <  4:304380/137‑1 (MQ=255)                           cGACCCCCCCACGCTAGGCGTTTCCGAACAGCAGGCTGTCGGCGCTGGCATCGATCAGGATAGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAcc                                                                                                        >  4:297121/1‑136 (MQ=255)                               caccGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCg                                                                                                    <  1:135284/137‑1 (MQ=255)                                    cACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGcaca                                                                                               <  6:288452/137‑1 (MQ=255)                                    cACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGcaca                                                                                               <  8:115718/137‑1 (MQ=255)                                     aCGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACAt                                                                                              >  6:118153/1‑137 (MQ=255)                                      cGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATg                                                                                             >  8:165627/1‑137 (MQ=255)                                           ggCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                        >  8:122301/1‑137 (MQ=255)                                           ggCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                        <  5:265432/137‑1 (MQ=255)                                           ggCGTTGCCGAACAGCAGGATGTCGGAGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                        <  6:132639/137‑1 (MQ=255)                                           ggCGTTGACGATCATCAGGATGTCGGCGCTGGAATGGATCAGGATCGAGTGACGAAACTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                        <  1:286958/137‑1 (MQ=255)                                            gCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTa                                                                                       <  4:12665/137‑1 (MQ=255)                                              ggtGCCGAACAGCAGGATGTCTGCGCTGGCATCGATCATGATGGAGTGACGAACCTTGTCCTGCCTGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAgg                                                                                     <  6:158414/135‑1 (MQ=255)                                               ttGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                        <  4:224001/133‑1 (MQ=255)                                               ttGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                        >  3:224001/1‑133 (MQ=255)                                                       cagcagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGc                                                                            <  3:32561/137‑1 (MQ=255)                                                         gcagTATGTCGGCGCTGTCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGTCACATGCTTGTAGGCCTCCTTGCAg                                                                          <  5:104174/137‑1 (MQ=255)                                                         gcagGATGTCGGCGCTGTCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                          <  5:95805/137‑1 (MQ=255)                                                         gcagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                          <  3:80616/137‑1 (MQ=255)                                                         gcagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                          <  5:68101/137‑1 (MQ=255)                                                         gcagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                          <  6:108834/137‑1 (MQ=255)                                                         gcagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                          <  7:216801/137‑1 (MQ=255)                                                          cagGATTTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTTGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                         <  8:67554/137‑1 (MQ=255)                                                          cagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                         <  4:140693/137‑1 (MQ=255)                                                          cagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                         >  7:225376/1‑137 (MQ=255)                                                          cagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                         <  3:179793/137‑1 (MQ=255)                                                          cagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                          >  4:308119/1‑136 (MQ=255)                                                          cagGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                          <  3:308119/136‑1 (MQ=255)                                                                  cGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCta                                                                 >  7:282674/1‑137 (MQ=255)                                                                    gcgcTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCGGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCtata                                                               <  3:127947/137‑1 (MQ=255)                                                                    gcgcTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCtata                                                               <  6:313316/137‑1 (MQ=255)                                                                     cgcTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                         <  7:250751/126‑1 (MQ=255)                                                                     cgcTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                         >  8:250751/1‑126 (MQ=255)                                                                          gCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAg                                                         >  1:137544/1‑137 (MQ=255)                                                                            aTCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGc                                                       <  1:187797/137‑1 (MQ=255)                                                                             tCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCGGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCt                                                      >  1:267251/1‑137 (MQ=255)                                                                             tCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCt                                                      <  2:306531/137‑1 (MQ=255)                                                                              cGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTg                                                     <  7:122301/137‑1 (MQ=255)                                                                                   aGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACg                                                <  6:1213/137‑1 (MQ=255)                                                                                        cGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCg                                           <  4:237434/137‑1 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                         <  3:1899/70‑1 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                         >  4:1899/1‑70 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTTTAGGCCTCCTTGCAGCGCTGGCTATACACAAGGCTGGCACGCTCCGGACgg                                      >  5:285366/1‑137 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACgg                                      >  3:116208/1‑137 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACgg                                      >  7:176602/1‑137 (MQ=255)                                                                                             gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACgg                                      >  7:14596/1‑137 (MQ=255)                                                                                                  aCCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGcc                                 >  2:192234/1‑137 (MQ=255)                                                                                                  aCCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGcc                                 >  8:85327/1‑137 (MQ=255)                                                                                                  aCCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGACCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGcc                                 >  6:37821/1‑137 (MQ=255)                                                                                                  aCCTTGTCCTGACAGTAGTCGTAGGGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGcc                                 <  5:341200/137‑1 (MQ=255)                                                                                                     ttGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggc                              <  1:121367/137‑1 (MQ=255)                                                                                                     ttGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggc                              <  4:22049/137‑1 (MQ=255)                                                                                                         ccTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccagg                          >  1:289282/1‑137 (MQ=255)                                                                                                           tGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCtt                                                                                          <  6:35667/71‑1 (MQ=255)                                                                                                           tGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCtt                                                                                          >  5:35667/1‑71 (MQ=255)                                                                                                           tGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcc                        >  8:79421/1‑137 (MQ=255)                                                                                                            gCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcca                       <  7:92251/137‑1 (MQ=255)                                                                                                            gCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcca                       <  5:180541/137‑1 (MQ=255)                                                                                                            gCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcca                       <  4:30686/137‑1 (MQ=255)                                                                                                              cAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGccacc                     <  8:225376/137‑1 (MQ=255)                                                                                                                         gTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAgg          >  6:325274/1‑137 (MQ=255)                                                                                                                         gTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAgg          <  1:225176/137‑1 (MQ=255)                                                                                                                         gTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGATATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAgg          <  7:235234/137‑1 (MQ=255)                                                                                                                          tGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGc         >  4:123973/1‑137 (MQ=255)                                                                                                                            ggCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCAt       <  7:165627/137‑1 (MQ=255)                                                                                                                                 tGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACACGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCAtcgtcg  >  3:184600/1‑137 (MQ=255)                                                                                                                                    |                                                                                                                                      CCCGTTGGACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGGATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCGGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCATCGTCG  >  ACB122/5532964‑5533228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑