Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	ACB122	4,257,351	+CGCGGCCG	100%	intergenic (‑280/+8)	CJ019_03991 ← / ← CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	ACB122	= 4257351	1 (0.020)	49 (0.790) +CGCGGCCG	32/216	NT	98.1%	intergenic (‑280/+8)	CJ019_03991/CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein
	?	ACB122	4257352 =	1 (0.020)					intergenic (‑281/+7)	CJ019_03991/CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein

CGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB122/4257218‑4257351 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGGCG  >  ACB122/4257352‑4257481                                                                                                                                       ||||||||                                                                                                                                   CGCCCCATCAATGCATCCACTAAAACCCGAAAATCCCACAGAATCCGCACCTACCGAACAAAATGCATTGCCCTACCACGCCGCCCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCC                                                                                                                                           <  1:110363/135‑1       agCAATGTATCAAATAAAAACCGATACTCGCACATAATCCGCACCTACCGAACAAATTGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTATTTAAAAACATAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                     <  8:41708/133‑1       ATCAATGGATCCACTAAAACCCGAAAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                     <  1:291116/135‑1       ATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCC                                                                                                                                     <  8:441200/135‑1            TGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCC                                                                                                                                >  7:312514/1‑135               ATCCACTAAAAACCCATAACCGCACAGAAACCGCACCAACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGT                                                                                                                              <  1:385481/134‑1                 CCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTT                                                                                                                           >  6:213631/1‑135                 CCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCAATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCCTCT                                                                                                                            >  6:253396/1‑134                  CACTAACAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCCCGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTA                                                                                                                          <  8:312514/135‑1                   ACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTAC                                                                                                                         <  8:343363/135‑1                   ACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGGCTTAC                                                                                                                         >  1:248173/1‑135                      AAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTTACCTCCCACGCCGACCGCAGCCTTAATGGATCAATTAAATAAAAACAAACCCCGGAGAGCCTCCCGCGGCAGCCCCCTCATAagca                                                                                                                      >  8:177406/1‑131                       AAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACt                                                                                                                     >  7:41708/1‑134                             GATAATCGCACAGACTCCGCACCAACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGAGCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCA                                                                                                               <  6:157124/135‑1                                        AGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTT                                                                                                     >  1:343299/1‑134                                          acgCCGCACCTACCGAAAAACATGCCTTGACCTACCACGCCGACCGCATCCTTAATGGATCCATTAAATAAACACAAACCCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCAT                                                                                                  <  7:236448/132‑1                                          AATCCGCACCTACCCAACAAAATGCATTGACCTACCCCGCCGACCGCAGCCTTAATGGATCCTTAAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCAT                                                                                                  <  4:433350/135‑1                                               GCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCG                                                                                             >  2:39693/1‑135                                                       CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCCTGCCCGCGTCTGCC                                                                                     >  6:457402/1‑135                                                       CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCC                                                                                     >  3:92032/1‑135                                                       CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCCTCTGCC                                                                                     >  2:304386/1‑135                                                       CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCCTCTTACGACGAGGGCAGGTTGACTTACAAGCCCGCGTCatcc                                                                                     >  4:420104/1‑131                                                         AACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGC                                                                                   <  4:370746/135‑1                                                         AACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCC                                                                                     >  3:370746/1‑133                                                         AACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCC                                                                                     >  3:139719/1‑133                                                                TGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCG                                                                            >  4:89873/1‑135                                                                   ATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCC                                                                          <  1:39693/134‑1                                                                   ATTCACCTCCCACGCCGACCGCAGCCTTAATGGATCCATAAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCC                                                                          <  1:307190/134‑1                                                                      GACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCACCCGCGGCCGCCCCCTCTTACGACGAGGGCACGATGACTTAAATGCCCGCGTATCCCCCCCCCCCCCCAAAc                                                                      >  2:191843/1‑134                                                                            CCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                                <  4:158263/135‑1                                                                              ACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGC                                                              >  6:124580/1‑135                                                                               CGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGC                                                              >  7:394080/1‑134                                                                                      CGCCGCCTTAATGGATCCATAAAATAAAAACAAACCCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                      <  6:72837/135‑1                                                                                      CGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                      <  8:394080/135‑1                                                                                      CGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                      <  8:126256/135‑1                                                                                      CGCAGCCTTAATGGAACCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                      <  3:90667/135‑1                                                                                      CGCAGCCTTAAGGGATCCATTAAATAAAACCACAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                      <  2:263200/135‑1                                                                                              TAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCA                                              >  3:410186/1‑135                                                                                                    ccCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGATGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGC                                         <  5:454917/132‑1                                                                                                      aaataAAAAAAAACCAAAACCCGCAGCGCCGCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGT                                       <  5:253396/129‑1                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATTCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCT                                    >  6:119045/1‑135                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATTCCCGCGTCTGCCGCAGCCCCCCCAAAGGTTTCGGCATAGCGCAAGTTGGCATTGGCGTG                                      >  1:49263/1‑133                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCT                                    >  2:124818/1‑135                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCT                                    >  8:350593/1‑135                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCT                                    >  1:265262/1‑135                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCCTCGTACGACGAGGGGAGGATGACTTTAATTCCCGCGTCAGCCGCCGCCCCCCCCAAGGTTTCGGCCTTGCGCAAGGGTGCATTGGCGTGCT                                    >  6:12013/1‑135                                                                                                          TAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCG                                  >  8:226302/1‑135                                                                                                             ATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCCTAGCGCAAGGTGGCCTTGGCGTGCTCGC                                 >  4:104190/1‑133                                                                                                                  AACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGAT                          >  3:228823/1‑135                                                                                                                    CAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                                <  8:113160/95‑1                                                                                                                    CAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCG                                                                >  7:113160/1‑95                                                                                                                           CGGAGAGCCTCCCGCGGCCGCCCCGTGTTACGACGAGGGCAGGATGACTTTAATGCCCGCGTCTGCCGCAGCACCCCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGAGTGCTCGCGCATGATCGCTTCGtc                 >  1:316139/1‑133                                                                                                                           CGGAGAGCCTCCCGCGGCCGCCCCGGCTTCCGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTGCGGC                 <  6:447764/135‑1                                                                                                                             GAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGC               <  4:92032/135‑1                                                                                                                                       CGCGGCCGCCCCGTCTTAAGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCCGCCCCGCCAAACGTTTCGGCATAGCGCAACGTGGCATTGGCGTGCTCGCGCATGATAGCTACGGGGCGCGCACCCag     >  8:402021/1‑133                                                                                                                                          cggCGCCCCGTCCTCCGACGAGGGCAGGATGACTTTCATGCCGGCGTCAGCCGGAGCACCGCCAAAGGTTGCGGCATAGCGCAATGTGGCCTTGGCGTGCTGGAGCATGATCGCTTCGGCGCGGGCACCCTGGCG  <  7:228821/132‑1                                                                                                                                       ||||||||                                                                                                                                   CGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB122/4257218‑4257351 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGGCG  >  ACB122/4257352‑4257481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.