New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	ACB122	5489163 =	86 (1.300)	3 (0.050)	3/212	NT	3.7%	coding (154/1317 nt)	sir_2	Sulfite reductase [ferredoxin]
	?	ACB122	= 5489159	80 (1.330)					coding (158/1317 nt)	sir_2	Sulfite reductase [ferredoxin]
Rejected: Frequency below/above cutoff threshold.

GCCGAAGCCCCCACTGTCATCCCTCGTCCCTCGGCCTGCCCGGGGTTGTGGCGCATCGTCAGTGCCCGTGATGGCGGTATCTGCCGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTGGCGGCGGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB122/5489307‑5489163 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcggcggcggAGCGTTTTGCTGGCGGCGTGATCGAGGCCACCAACCGTGGCAACCTGCAGATTCGTGGCATTGGCAGCGAGCATCGCGCTTTGGTCGAGGCACTGCTCGCAGCCGGCCTGGGACCACGCGATGCCG  <  ACB122/5489159‑5489034                                                                                                                                                                                                                                                                                 GCCGAAGCCCCCACTGTCATCCCTCGTCCCTCGGCCTGCCCGGGGTTGTGGCGCATCGTCAGTGCCCGTGATGGCGGTATCTGCCGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTG                                                                                                                                          >  4:423406/1‑135   CGAAGCCCCCACTGTCATCCCTCGTCCCTCGGCCTGCCCGGGGTTGTGGCGCATCGTCAGTGCCCGTGATGGCGGTATCTGCCGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTGGC                                                                                                                                        >  2:106491/1‑135   CGAAGCCCCCACTGTCATCCCTCGTCCCTCGGCCTGCCCGGGGTTGTGGCGCATCGTCAGTGCCCGTGATGGCGGTATCTGCCGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTGGC                                                                                                                                        >  2:297434/1‑135    GAAGCCCCCACTGTCATCCCTCGTCCCTCGGCCTGCCCGGGGTTGTGGCGCATCGTCAGTGCCCGTGATGGCGGTATCTGCCGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTGGCG                                                                                                                                       >  5:104939/1‑135    GAAGCCCCCACTGTCATCCCTCGTCCCTCGGCCTGCCCGGGGTTGTGGCGCATCGTCAGTGCCCGTGATGGCGGTATCTGCCGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTGGCG                                                                                                                                       >  5:223975/1‑135    GAAGCCCCCACTGTCATCCCTCGTCCCTCGGCCTGCCCGGGGTTGTGGCGCATCGTCAGTGCCCGTGATGGCGGTATCTGCCGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTGGCG                                                                                                                                       >  7:301439/1‑135     AAGCCCCCACTGTCATCCCTCGTCCCTCGGCCTGCCCGGGGTTGTGGCGCATCGTCAGTGCCCGTGATGGCGGTATCTGCCGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTGGCGG                                                                                                                                      <  3:195302/135‑1        CCCCCACTGTCATCCCTCGTCCCTCGGCCTGCCCGGGGTTGTGGCGCATCGTCAGTGCCCGTGATGGCGGTATCTGCCGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTGGCGGCGG                                                                                                                                   >  3:103840/1‑135                                                                                 CTGCCGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTGGCGGCGGCGGAGCGTTTTGCTGGCGGCGTGATCGAGGCCACCAACCGTGGCAACCTGCAGATTCGTGGCATTGGCAGCGA                                                          >  8:52635/1‑135                                                                                     CGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTGGCGGCGGCGGAGCGTTTTGCTGGCGGCGTGATCGAGGCCACCAACCGTGGCAACCTGCAGATTCGTGGCATTGGCAGCGAG                                                         <  7:52635/132‑1                                                                                                                 TGGCCGAGCAGGCTGAAGCCGTGGCGGCGGCGGAGCGTTTTGCTGGCGGCGTGATCGAGGCCACCAACCGTGGCAACCTGCAGATTCGTGGCATTGGCAGCGAGCATCGCGCTTTGGTCGAGGCACTGCTCGC                            <  5:219130/133‑1                                                                                                                                       GGCGGCGGCGGAGCGTTTTGCTGGCGGCGTGATCGAGGCCACCAACCGTGGCAACCTGCAGATTCGTGGCATTGGCAGCGAGCATCGCGCTTTGGTCGAGGCACTGCTCGCAGCCGGCCTGGGACCACGCGATGC    <  1:142856/135‑1                                                                                                                                         CGGCGGCGGAGCGTTTTGCTGGCGGCGTGATCGAGGCCACCAACCGTGGCAACCTGCAGATTCGTGGCATTGGCAGCGAGCATCGCGCTTTGGTCGAGGCACTGCTCGCAGCCGGCCTGGGACCACGCGATGCCG  <  2:277189/135‑1                                                                                                                                                                                                                                                                                 GCCGAAGCCCCCACTGTCATCCCTCGTCCCTCGGCCTGCCCGGGGTTGTGGCGCATCGTCAGTGCCCGTGATGGCGGTATCTGCCGCATCAAGCTGCCGGGTGGCCTGCTGCTGGCCGAGCAGGCTGAAGCCGTGGCGGCGGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB122/5489307‑5489163 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcggcggcggAGCGTTTTGCTGGCGGCGTGATCGAGGCCACCAACCGTGGCAACCTGCAGATTCGTGGCATTGGCAGCGAGCATCGCGCTTTGGTCGAGGCACTGCTCGCAGCCGGCCTGGGACCACGCGATGCCG  <  ACB122/5489159‑5489034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.