Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	365,722	A→C	22.9%	intergenic (+92/‑87)	stcD_1 → / → CJ019_00339	putative N‑methylproline demethylase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	365,722	0	A	C	22.9%	95.1 / 4.7	67	intergenic (+92/‑87)	stcD_1/CJ019_00339	putative N‑methylproline demethylase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/19);  new base C (3/14);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 4.02e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTACCCATCCTGCTGTTCGCTGCCCTTGGCCTG  >  ACB122/365591‑365856                                                                                                                                     |                                                                                                                                        ggcgATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGCCCGGACGc                                                                                                                                      >  2:285259/1‑135 (MQ=255)  gcgATCTACGACGCCTTGCGCCTGGGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGATTACCCGCGAATGCGGCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCa                                                                                                                                     <  2:81490/135‑1 (MQ=255)   cgATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCCTCAGCACTGACAACAC‑TGTTGCCTGACCGGCCGCAt                                                                                                                                    >  2:443879/1‑135 (MQ=255)           gacgCCTTGCGCCTGTGCAAGGACTTCTGATCGTTACCAGTCGCCGCCAGCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCTAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGCCGCATT‑CGCGGGt                                                                                                                           <  8:360441/135‑1 (MQ=255)             cgCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGa                                                                                                                         <  8:12542/135‑1 (MQ=255)             cgCCTTGCGCCTGTGCAAGGACTGCTGCGCGTTAACCGTCGCCCCCATCGGGCGCGATCCCTGTAGGAGCGGGTTAACCCGCGAATGCGGCCGCACTCACAACAC‑TGTTGCCTCCCCGGCCGCATT‑CGCGGGTGa                                                                                                                         <  6:346041/135‑1 (MQ=255)             cgCCTTGCGCCTGGGCAAGGACTGCTGAGCGTTAACCGGCGCCGCCATCGGGCGCGATCCCTGAAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTGGCCTGACCGGACGCATT‑CGCGGGTGa                                                                                                                         <  3:333049/135‑1 (MQ=255)                 ttGCGCCTGTGCAAGGAATTCTGATCGTAAACAGGCGCCGCCATCGGGCGCTATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTTCCTGACCGGACGCATT‑CGCGGGTTAAccc                                                                                                                     <  6:362178/135‑1 (MQ=37)                   gcgcCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCCCTGACAACAC‑TGTTGCCTGACCGGACGCATT‑AGCGGGGGACCCCGc                                                                                                                   >  4:391872/1‑135 (MQ=255)                   gcgcCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑AGCGGGTGAACCCGc                                                                                                                   >  5:369079/1‑135 (MQ=255)                     gcCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCCCTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGCACCCGctc                                                                                                                 >  6:214630/1‑135 (MQ=255)                     gcCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCCCTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGctg                                                                                                                 >  6:138079/1‑134 (MQ=255)                             aaGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGCACCCGCTCTTACATgg                                                                                                         >  3:176323/1‑135 (MQ=255)                             aaGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATgg                                                                                                         >  1:128077/1‑135 (MQ=255)                             aaGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCCAATGCGTCAGCACTGACAACAC‑AGTTGCCTGACCGGCCGCAAT‑AGCCGGGGAACCCCCTCTTAAAttg                                                                                                         >  6:40161/1‑133 (MQ=255)                             aaGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACACGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCCTT‑CGCGGGTGAACCCGCTCTTACATgg                                                                                                         >  1:82494/1‑135 (MQ=255)                                  cTTCTGATCGTTAACAGTCGCCGCCATCGGGCTCGATCCCTGTAGTAGCGGGTTTACACGCGAATTCGTCAGCACTGACAACAC‑TGTTTCCTGACCGGACGCATT‑CGCGTGTGAACCCGATCTTACATGGGGCCa                                                                                                    >  4:267993/1‑135 (MQ=255)                                    tCTGATCGTAAACCGGCGCCGCCATCGGGCGCGATCCCTGTAGGCGCGGGTTTCCCCGCTAAGGCGGCACCACTGACACCAC‑TGTTGCCTGCCCGGACCCATG‑GGCGGGTGAACCCGCTCTTACATGGGGCCAGt                                                                                                  <  6:179830/135‑1 (MQ=255)                                       gATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCCCTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGtctc                                                                                               >  7:235212/1‑135 (MQ=255)                                       gATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATTGGGCCAGtcta                                                                                               >  2:308403/1‑134 (MQ=255)                                               acggTCGCCGCCAGCGGGCGCTATCCCTGATGGCGGGGGTATACCCGCGAATGCGGCAGCACTGACAACAC‑TGTTGCCTCACCGGCCCCATG‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCctct                                                                                        <  2:82494/131‑1 (MQ=255)                                                cAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACCCCAC‑TGTTGCCTGACCGGACGCAAT‑CGCCGGTGAACCCGCTCTTACATGGGGCCCGTCTCGCCctct                                                                                        >  4:134944/1‑133 (MQ=39)                                                cAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGGCGCATT‑AGCCGGTGCACCCCCGCATAAAAGGGGCCCGGGTCGCCCACTgg                                                                                      >  1:407746/1‑135 (MQ=37)                                                    cgccgcCTGCGGGCGCGACCCCGTTCGGCGCGGGTTTACCCGCTAATGCGTCAGCACTGACAACAC‑TGTGGCCTCACCGGCCGCTTG‑CGGGGGTTAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCgg                                                                                  <  3:375774/135‑1 (MQ=255)                                                        gccATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGCCCGGACGCATT‑CGCCGGGGAACCCGCTGTTTCCCCGGGCCAGTCTCGCCCTGTGGGCGGGGCa                                                                              >  2:154611/1‑135 (MQ=37)                                                        gccATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGgcg                                                                                >  7:123831/1‑133 (MQ=255)                                                              gggCGCGATCCCTGTAGGAGCGGGTTTACCCGCTAATGCGTCAGCACTGACAACAC‑TGTGGCCTGCCCGGACGCATG‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATc                                                                        <  3:137348/135‑1 (MQ=255)                                                                        ccTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACCC‑TGTTGCCTGCCCGGCCGCCTT‑CGCGGGGGAACCCGCTCGTCCATGGGGGCAGTCGGGCCCTCTGGTCGGCGCCAGGATCCCGCTGGTtg                                                              >  1:280307/1‑135 (MQ=25)                                                                          tgacgcaGCGGGTATCCCCGCGAAGGCGGCACCACTGACAACAC‑TGTTGCCTCCCCGCCCCCTTG‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTtgtg                                                            <  1:285259/129‑1 (MQ=255)                                                                          tGTCGGCGCGGGTTCCCCCGCTATTGCGACAGCACTCACACCAG‑TGTGGCCGGACC‑GCCCCATTGCGGGGGTAAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTtgtg                                                            <  5:316571/135‑1 (MQ=255)                                                                          tGTCGGAGCGGGTTTACCGGCGAATGCGTCAGAACTGACAACAC‑TGTTGCCTGCCCGGACGCATT‑GGCGGGTTAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTtgtg                                                            <  6:187332/135‑1 (MQ=255)                                                                          tGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACCC‑TGTTGCCTGACCGGACGCATT‑CGGGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTtgtg                                                            <  3:389479/135‑1 (MQ=255)                                                                          tGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTGGCCTGACCGGACGCATG‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTtgtg                                                            <  4:351903/135‑1 (MQ=255)                                                                                 gCGGGTTTCCCCGCTATGGCGTCCGCACTGACAACAC‑TGTGGCCTGCCGGGCCGCATT‑CGCGGGTTAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCt                                                     <  5:195476/135‑1 (MQ=255)                                                                                 gCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCt                                                     <  2:91348/135‑1 (MQ=255)                                                                                     gTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTTGGAGCCTCCCa                                                 >  5:319763/1‑135 (MQ=255)                                                                                       ttACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGCACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAAGCCCGCTGTTTGTGGGGGCCTCCCCTg                                               >  1:9106/1‑135 (MQ=38)                                                                                       ttACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑AGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCGCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATg                                               >  6:383610/1‑135 (MQ=255)                                                                                         aCCCGCGAATGCGTCAGCACTGCCCACAC‑TGTTGCCTGCCCGGACGCATT‑CGCGGGGGCACCCGCGCGTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAAGCCCGCGGGTGGTGGGCGCCTCCCagggt                                             >  4:47346/1‑131 (MQ=255)                                                                                          ccggcgATTGCGTCACCACTCACACCCC‑TGTGGCCTCCCGGCCCGCATG‑CGCGGGTTAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGtt                                             <  7:238451/131‑1 (MQ=255)                                                                                          cccGCTAATGCGTCCGCAGTGACAACAC‑TGGTGCCTGCCCGGCCCCATG‑CGGGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGtt                                             <  6:182718/134‑1 (MQ=255)                                                                                          cccGCCAATGCGACAGAACT‑TCAAAACTTGTTGCTTGCCGTGACCCATT‑CGCGGTTCAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGtt                                             <  1:289554/134‑1 (MQ=255)                                                                                           cgtggAAGGCGTCATCAATGACAACAT‑TGTTCCCTGCCCGGCCGCATT‑CGGGGGTTAACCAGCTCTTACATGTGGCCAGTCTCGCCATCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTg                                            <  4:334484/130‑1 (MQ=255)                                                                                           ccGCTAATGCGTCAGCACTGACAACAC‑GGTGGCGGGCCCGGACGCATT‑GGGGGTTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGa                                           <  2:115864/135‑1 (MQ=255)                                                                                             gcgAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCGCCCATGTTGAac                                         >  6:228024/1‑135 (MQ=255)                                                                                             gcgAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGGTTGTGGGAGCCTCCCATGTTGAac                                         >  4:76054/1‑135 (MQ=255)                                                                                               tattgggTCCGCACTGACACCAC‑TGTGGCGTCCCCGTCCCCTTT‑CGCGGGTTACCCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAacac                                       <  4:427857/129‑1 (MQ=255)                                                                                                  tGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCt                                    <  8:123831/135‑1 (MQ=255)                                                                                                     gtaaGCACTGACAACAC‑TGTTGCCTCCCCGGACGCATG‑GGGGGGGGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTc                                  <  4:40751/131‑1 (MQ=255)                                                                                                       cAGCACTGACAACAC‑TGTTGCCTGACCGGCCGCAGG‑CGCGGGTGAACCGGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTAc                               <  7:461503/135‑1 (MQ=255)                                                                                                         gCAGTGACAACAC‑TGTTGCCGGACCGGAGGCATT‑CGGGGGTGAACCGGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTAccc                             <  4:176323/135‑1 (MQ=255)                                                                                                                caacaC‑TGTTGCCTGACCGGACGCATT‑AGCGGGTGAACCCGCTCTTACATGGGGGCACTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGTGCCTCCCATGTTGAAGACCCTTCGACCcctcct                        >  2:430966/1‑133 (MQ=255)                                                                                                                  acac‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTACCCATCctgctg                    >  7:260200/1‑135 (MQ=255)                                                                                                                  acac‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCAGGTTGAACACCCTTCTACCCATCctgctg                    >  1:324348/1‑135 (MQ=255)                                                                                                                  acac‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGGTTTTGGGTGGCTGCCATGTTGAACACCCTTCTTCCCATCctgcgg                    >  7:167975/1‑133 (MQ=255)                                                                                                                         ttGCCTGCCCGGCCGCTTG‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTACCCATCCTGCTGTTCGCt              <  7:296174/135‑1 (MQ=255)                                                                                                                           gCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTtgtg                                                            >  6:318127/1‑87 (MQ=255)                                                                                                                           gCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTtgtg                                                            <  5:318127/87‑1 (MQ=255)                                                                                                                           gCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCGGTTTGTGGGCGCCTCCCATGTTGAACACCCTTCTACCCATCCTGCTGTTCGCTGc            >  8:224534/1‑135 (MQ=255)                                                                                                                            cctccccGGCCGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGGCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCATCTACCCATCGTGCTGTTCGCTGc            <  4:440253/129‑1 (MQ=255)                                                                                                                            ccTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTACCCATCCTGCTGTTCGCTGc            <  4:234920/134‑1 (MQ=255)                                                                                                                             cTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTACCCATCCTGCTGTTCGCTGccc          >  3:392303/1‑135 (MQ=255)                                                                                                                               gACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCa                                                                      <  8:382441/73‑1 (MQ=255)                                                                                                                               gACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCa                                                                      >  7:382441/1‑73 (MQ=255)                                                                                                                                 ccGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTACCCATCCTGCTGTTCGCTGCCCTTgg      >  3:326717/1‑135 (MQ=255)                                                                                                                                   ggACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTAccc                             <  3:107166/110‑1 (MQ=255)                                                                                                                                   ggACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTAccc                             >  4:107166/1‑110 (MQ=255)                                                                                                                                     aCGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTACCCATCCTGCTGTTCGCTGCCCTTGGCCTg  <  6:369079/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        GGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACAC‑TGTTGCCTGACCGGACGCATT‑CGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAACACCCTTCTACCCATCCTGCTGTTCGCTGCCCTTGGCCTG  >  ACB122/365591‑365856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑