Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,974,886	A→C	21.6%	V154G (GTC→GGC)	CJ019_03752 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,974,886	0	A	C	21.6%	92.6 / 4.0	60	V154G (GTC→GGC)	CJ019_03752	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/13);  new base C (7/8);  total (39/21)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGCCTTCGGCGTCGATCAGGTCGGCCTTGTTCAACACCACCAGGTCGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAATGGCTTGGGCAG  >  ACB122/3974755‑3975016                                                                                                                                    |                                                                                                                                   ccAGGCCTTCGGCGTCGATCAGGTCGGCCTTGTTCAACACCACCAGGTCGGCGCTGGCCAGCTGGTCAGCGCACAGCTCGTGCCGGGGCGATTCATGGTCCAGGGTAGGGTCGAGCTTGCGCTGGGCATCGCCCt                                                                                                                                 >  1:269188/1‑135 (MQ=255)       cTTCGGCGTCGATCAGGTCGGCCTTGTTCAACACCACCAGGTCGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCg                                                                                                                           >  6:382858/1‑135 (MQ=255)                        gcGGCCTTGTTCACCCCCACCCGGGCGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGGCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTg                                                                                                          <  1:409608/134‑1 (MQ=255)                          ggCCTTGTTCAACACCACCAGGTCGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTGCAGGTTAGGGTCGAGCTTGCGCTGGGCAACGACCTGGTGCCGGTTGGCGGCAAAGGTg                                                                                                          >  4:450500/1‑133 (MQ=255)                                         caccaGGTCGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCCTCGACCTGTTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCccggccgg                                                                                         >  6:461065/1‑135 (MQ=255)                                         caccaGGTCGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCgg                                                                                         >  8:381268/1‑135 (MQ=255)                                         caccaGGTCGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCgg                                                                                         >  6:329318/1‑135 (MQ=255)                                         caccaGGTCGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCGGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCgg                                                                                         >  1:384371/1‑135 (MQ=255)                                            cggggcGGCGCGGGCCCGGGGGCCAGCGAACAGCGCGGGCGGGGGCGATTCAGGCGCCTGGTTCGGGGCGCGCTGGCGCGGGCCAGCGCCCGGGGCCGGGTCGGCGCCAACGGGGCCAGCGGCCACGGCCGGGCt                                                                                      <  2:172916/130‑1 (MQ=255)                                              ggTCGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGGGTGCGCTGGGGCTCGCCCGGGTCCGGGGTGGCGGCCAAGGTGCCAGCGGCCACCGCCGGGcggg                                                                                    >  5:282370/1‑132 (MQ=255)                                              ggTCGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGCCCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCCCGGCCGGGCTGt                                                                                    >  1:186345/1‑135 (MQ=255)                                                tCGGGGCGGCCCAGGGGGGCAGCGAACCGCGCGTGCCGGGCCGATCCAGGGCCCAGGTTCGGGGGGGGCTGGCGCGGGGAAGCGCGGTGGTCCGGTTAGGCGGAAAAGGGGCCAGCGGCCACGGCCGGGCTGTCg                                                                                  <  2:154290/135‑1 (MQ=255)                                                tCGGCGCTGGCCAGCTGGTCAGCGAACAGCGCGTGCAGGGGCGATTCATGGGCCAGGTTCGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGGGCCAGCGGCCACGGCCGGGCTGTCg                                                                                  <  7:344261/135‑1 (MQ=255)                                                tCGGCGCGGGCCAGAGGGTCAGCGAACAGCGCGCGCAGGGGCGATTCATGGCCACGGTGAGGGGCGAGCTTGCGCGGGGCACCGACCTGGTCCGGGTCGGCGGAAACGGTGCCAGCGGCCACGGCCGGGCTGTCg                                                                                  <  4:262777/135‑1 (MQ=255)                                                 cGGCGCTGGCCAGCTGGTCAGCGACCAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGCCCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGa                                                                                 >  7:444998/1‑135 (MQ=255)                                                 cGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGGGCAGGGGCGATTCATGGTCCAGGTTAGGGGCGAGCTTGCGCGGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGa                                                                                 <  7:174505/135‑1 (MQ=255)                                                    cgcTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGCCCa                                                                              >  3:169464/1‑135 (MQ=255)                                                      cTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGCCCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCCCGGCCGGGCTGTCGACCACg                                                                            >  3:2590/1‑135 (MQ=255)                                                            aGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGCCCACGGTAATg                                                                      >  5:176653/1‑135 (MQ=255)                                                                 gcctgCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTCGGGTCGAGCTGGCGCGGGGCATCGACCTGGGCCGGGTCGGCGGCAACGGGGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGc                                                                 <  4:169464/131‑1 (MQ=255)                                                                 gTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCGGGGTAGGGGGCAATGGTGCCAGCGGGCACGGCGGGGCTGTCGCCCACGGGTATGagc                                                                   >  7:175093/1‑131 (MQ=255)                                                                 gTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGCCCCCGGTAATGAcc                                                                   >  2:332281/1‑133 (MQ=255)                                                                 gTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGc                                                                 >  1:299924/1‑135 (MQ=255)                                                                   ctgctaaCAGCGCGTGCAGGGGCGATGCAGGGGCCCGGTTCGGGGCGCGCTGGCGCGGGGCAGCGCCCGGGTCCGGGTCGGCGGCAAAGGGGCCCGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCAt                                                               <  2:186345/130‑1 (MQ=255)                                                                   ccgCGAACAGCTCGTGCCGGGGCGTCTCAGGGGCCCGGTCTGGGTCGAGGTTGCGCGGGGCATCCCCCGGGGCCGGGTCGGCGCCAACGGGCCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCAt                                                               <  2:299924/133‑1 (MQ=255)                                                                   ccgCGAACAGCGCGTGCCGGGGCTATTCTGGGCCCCGGTTTGGGCCGCGCTTGCGCGGGCCAGCGACCGGGGCCGGGTCGGCGCCAAAGGGCCCAGCGCCCACGGCCGGGCTGTCGACCACGGTAATGACCGCAt                                                               <  1:334524/133‑1 (MQ=255)                                                                   cAGCGACCTGCTCGGGCAGGGGTGATTCTTGGGCCTGGTTTGGGGCGCGCTTGCGCTGGGCATCGACCGGGTCGGGGTAGGCGGAAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCAt                                                               <  2:269188/135‑1 (MQ=255)                                                                   cAGCGAACCGCTCGGGCCGGGGCGATTCATGGGCCCGGTGCGGGGCGAGCTTGCGCGGGGCAGCGCCCTGGTCCGGGTAGGCGGCAAAGGGGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCAt                                                               <  4:374395/135‑1 (MQ=255)                                                                     gCGAACAGCTCGGGCTCGGGCTAAGCTGGGTCCAGGGGCGGGGCGCGCTGGCGCTGGGCTGCGCCGGGGGCCGGGTCGGCGCCACGGGGGCCAGCGCCCACGGCCGGGCTGTCGACCACGGTAATGACCGCAt                                                               <  2:240028/133‑1 (MQ=255)                                                                               cGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCCCGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCCgg                                                   >  2:84913/1‑135 (MQ=255)                                                                                   ccggggCGTTGCAGGGGCCTGGTTAGGGTCGAGCTTGCGCTGGGCAGCGCCCGGGTCGGGGTCGGCGGCAACGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGtt                                               <  8:99601/133‑1 (MQ=255)                                                                                        gCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGa                                          >  1:8847/1‑135 (MQ=255)                                                                                          gATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGAtt                                        >  7:279516/1‑135 (MQ=255)                                                                                          gATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGAtt                                        >  7:346518/1‑135 (MQ=255)                                                                                          gATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGAtt                                        >  4:211074/1‑135 (MQ=255)                                                                                                   tCCCGGGTAGGGGCGAGCGTGCGCTGGGCATCGACGGGGGCCGGGTCGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGGCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCAc                               <  6:403240/135‑1 (MQ=255)                                                                                                       ggTTAGGGTCGAGCTTGCGCTGGCCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGa                           >  4:166037/1‑135 (MQ=255)                                                                                                        gTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGCCGGTGCAGGCGTTGCGGATTTCCGGCCACTGGaa                          >  7:296462/1‑135 (MQ=255)                                                                                                        gTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCTGATTTCCGGCCACTGGaa                          >  7:106094/1‑135 (MQ=255)                                                                                                          tcggggCGAGCTGGCGCTGGGCATCGACCTGGGCCGGGTAGGCGGCAACGGGGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACg                        <  4:2590/133‑1 (MQ=255)                                                                                                                cgcgCTGGCGCGGGGCATCGACCGGGTCGGGTTAGGCGGCAAAGGGGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTa                  <  7:382045/132‑1 (MQ=255)                                                                                                                cGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGCCGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTa                  >  4:100889/1‑135 (MQ=255)                                                                                                                cGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTa                  >  7:350597/1‑135 (MQ=255)                                                                                                                cGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTa                  >  1:133437/1‑135 (MQ=255)                                                                                                                     ttGCGTGGGTCATCTACCTGGGCGGGGTAGGCGCCCAAGGGGCCAGCGCCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAAt             <  5:461065/135‑1 (MQ=255)                                                                                                                     ttGCGCTGGGCATCGCCCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAAt             >  6:65516/1‑135 (MQ=255)                                                                                                                     ttGCGCTGGGCATCGACCTGGTGCGGGTAGGCGGCAAACGTGCCAGCGGCCACGGCCGGGCTGTCGACCCCGGTAATGACCCCATCGCACGTGCAGGCGTTTCGGATTTCCGGCCACTGGAACGCTTGTACCAAt             >  6:247419/1‑135 (MQ=255)                                                                                                                     ttGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAAt             >  7:382781/1‑135 (MQ=255)                                                                                                                     ttGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAAt             >  1:331928/1‑135 (MQ=255)                                                                                                                     ttGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCATGTACCAAt             >  4:283039/1‑135 (MQ=255)                                                                                                                     ttGCGCGGGGCATCGACCGGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAAt             <  8:455168/135‑1 (MQ=255)                                                                                                                         gcTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAATGGCt         >  7:120885/1‑135 (MQ=255)                                                                                                                          cTGGGCCTCGCCCGGGGCCGGGTAGGCGGAAACGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAATGGCtt        <  7:22958/135‑1 (MQ=255)                                                                                                                           tGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAATGGCTTg       >  6:464065/1‑135 (MQ=255)                                                                                                                               cATCGACCTGGGCCGGGTCGGCGGCAAAGGGGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAATGGCTTGGGCa   <  6:176653/135‑1 (MQ=255)                                                                                                                                aTCGCCCTGGTCCGGGTAGGAGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGTTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGACCGCTAGTACCAATGGCTTGGGCAg  >  2:350272/1‑135 (MQ=255)                                                                                                                                aTCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCCTCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAATGGCTTGGGCAg  >  2:209671/1‑135 (MQ=255)                                                                                                                                aTCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAATGGCTTGGGCAg  >  7:105463/1‑135 (MQ=255)                                                                                                                                aTCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAATGGCTTGGGCAg  >  1:350919/1‑135 (MQ=255)                                                                                                                                aTCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAATGGCTTGGGCAg  >  4:426678/1‑135 (MQ=255)                                                                                                                                    |                                                                                                                                   CCAGGCCTTCGGCGTCGATCAGGTCGGCCTTGTTCAACACCACCAGGTCGGCGCTGGCCAGCTGGTCAGCGAACAGCTCGTGCAGGGGCGATTCATGGTCCAGGTTAGGGTCGAGCTTGCGCTGGGCATCGACCTGGTCCGGGTAGGCGGCAAAGGTGCCAGCGGCCACGGCCGGGCTGTCGACCACGGTAATGACCGCATCGACGGTGCAGGCGTTGCGGATTTCCGGCCACTGGAACGCTTGTACCAATGGCTTGGGCAG  >  ACB122/3974755‑3975016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑