Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,986,089 A→G 5.5% Y209H (TAT→CAT)  CJ019_03763 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,986,0890AG 5.5% 233.5 / 3.8 72Y209H (TAT→CAT) CJ019_03763hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (44/24);  new base G (2/2);  total (46/26)
Fisher's exact test for biased strand distribution p-value = 6.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

GACAATCGCCACGCCGGTACCGAAGGCCAGGCCGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAC  >  ACB122/3985964‑3986219
                                                                                                                             |                                                                                                                                  
gACAATCGCCACGCCGGTACCGAAGGCCAGGCCGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATGCACCACTTg                                                                                                                           >  4:160756/1‑135 (MQ=255)
gACAATCGCCACGCCGGTACCGAAGGCCAGGCCGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATGCACCACTTg                                                                                                                           >  4:168163/1‑135 (MQ=255)
gACAATCGCCACGCCGGTACCGAAGGCCAGGCCGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTg                                                                                                                           >  8:49115/1‑135 (MQ=255)
      cGCCACGCCGGTACCGAAGGCCAGGCCGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTc                                                                                                                     <  3:65107/135‑1 (MQ=255)
          aCGCCGGTACCGAAGGCCAGGCCGTCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTcggct                                                                                                                 <  3:314172/135‑2 (MQ=255)
           cGCCGGTACCGAAGGCCAGGCCGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTcggccg                                                                                                                >  6:39861/1‑135 (MQ=255)
            gCCGGTACCGAAGGCCAGGCCGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTcggccgg                                                                                                               <  3:380230/135‑1 (MQ=255)
               ggTACCGAAGGCCAGGCCGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATGCACCACTTGCGGGTCGGCCGGCTc                                                                                                            <  3:168163/135‑1 (MQ=255)
               ggTACCGAAGGCCAGGCCGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATGCACCACTTGCGGGTCGGCCGGCTc                                                                                                            <  3:160756/135‑1 (MQ=255)
                    cGAAGGCCAGGCCGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGGCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGa                                                                                                       <  8:317834/135‑1 (MQ=255)
                         gccaggccGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATATCTGGGCACATACACAACTTGCGGGTCGGCCGGCTCGATGACGAtg                                                                                                  >  8:249356/1‑135 (MQ=255)
                         gccaggccGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGAtg                                                                                                  >  7:149585/1‑135 (MQ=255)
                              gccggccACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGAtggtggt                                                                                             >  8:278329/1‑135 (MQ=255)
                                cggccACCCGCGGGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGAtggtggtgg                                                                                           <  8:328782/135‑1 (MQ=255)
                                             ggAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGTGCGGGATGCTGGc                                                                              >  5:104649/1‑135 (MQ=255)
                                              gcaCCGGGGTAGTACCTGGGCGGCGAGGCCGGGGAGGCCCAGGTGCCATAGGTGGTGGGCGGGTTAAACCTGGGCACCTACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGGGGGCGGGCTGCTGGcc                                                                             <  6:272387/133‑1 (MQ=255)
                                              gacccGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGcc                                                                             <  2:284087/132‑1 (MQ=255)
                                              gAACCGGGGTAGTACCTGGGCGGCGAGGCCGGGGAGGCCCAGGGGCCATAGGGGGGGGGCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGcc                                                                             <  6:277883/135‑1 (MQ=255)
                                               aaCCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCg                                                                            >  4:15524/1‑135 (MQ=255)
                                                 ccGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCt                                                                          >  4:405851/1‑135 (MQ=255)
                                                 ccGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGCTGGTCGGGTTATAGCTGGGCACATACACCACATGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCt                                                                          >  7:378916/1‑135 (MQ=255)
                                                       tagtaCATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCt                                                                    >  7:46698/1‑135 (MQ=255)
                                                            cATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGt                                                               >  8:355415/1‑135 (MQ=255)
                                                            cATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGt                                                               >  7:50199/1‑135 (MQ=255)
                                                                  cggcgAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGtgctgct                                                         >  7:77053/1‑135 (MQ=255)
                                                                  cggcgAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGtgctgct                                                         >  3:142466/1‑135 (MQ=255)
                                                                    gcgAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGtgctgctgc                                                       >  8:385620/1‑135 (MQ=255)
                                                                       aGGCCGGGTAGGCCCAGGTGCCATATGTGGTGGTCGGGTTATAGCTGGGCACATACACAACTTGCGGGTCGGCCGGCTCGATGACTATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCATTGGGTGCTGCTGCCgc                                                    >  7:390291/1‑135 (MQ=255)
                                                                       aGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgc                                                    >  3:317792/1‑135 (MQ=255)
                                                                       aGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgc                                                    >  5:93007/1‑135 (MQ=255)
                                                                       aGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgc                                                    >  5:365495/1‑135 (MQ=255)
                                                                       aGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgc                                                    >  2:112840/1‑135 (MQ=255)
                                                                       aGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgc                                                    >  4:68317/1‑135 (MQ=255)
                                                                       aGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCAgc                                                    >  5:63101/1‑135 (MQ=255)
                                                                       aGGCCGGGTAGGCCCAGGTGCCAGAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgc                                                    >  5:168982/1‑135 (MQ=255)
                                                                         gCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgctg                                                  <  1:112840/135‑1 (MQ=255)
                                                                            gggTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgctggcg                                               <  8:46698/135‑1 (MQ=255)
                                                                                 ggCCCAGGGGCCATCGGGGGGGGTCGGGTTATAGCGGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgctggcggctgg                                          <  5:386519/135‑1 (MQ=255)
                                                                                  gCCAAGGTGCCAAAGGTGGTGGGCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgctggcggctgg                                          <  2:328672/134‑1 (MQ=255)
                                                                                      aGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCgcag                                     <  1:41865/135‑1 (MQ=255)
                                                                                          gCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGc                                                                              >  1:412356/1‑90 (MQ=255)
                                                                                          gCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGc                                                                              <  2:412356/90‑1 (MQ=255)
                                                                                            cATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCgcaggtgca                                <  4:142466/134‑1 (MQ=255)
                                                                                               aGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGcaggtgcaggtg                            >  4:438956/1‑135 (MQ=255)
                                                                                               aGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGcaggtgcaggtg                            >  1:427632/1‑135 (MQ=255)
                                                                                                ggtggtggtCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCaggtgcaggtgc                           >  6:74437/1‑135 (MQ=255)
                                                                                                ggtggtggtCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCaggtgcaggtgc                           >  2:130906/1‑135 (MQ=255)
                                                                                                ggtggtggtCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGACTGGCGCaggtgcaggtgc                           >  3:337336/1‑135 (MQ=255)
                                                                                                     tggtCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCt                      <  7:315677/135‑1 (MQ=255)
                                                                                                       gtCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGc                    >  3:209587/1‑135 (MQ=255)
                                                                                                         cGGGTTATAGCTTGTCAAATACACCACTTGCGGGGCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCAc                  <  2:147508/135‑1 (MQ=255)
                                                                                                         cGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCCGGTGCGGTCTGCAc                  >  8:225447/1‑135 (MQ=255)
                                                                                                          gggTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACg                 >  7:282775/1‑135 (MQ=255)
                                                                                                          gggTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACg                 >  5:293271/1‑135 (MQ=255)
                                                                                                           ggTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACgg                <  1:332005/135‑1 (MQ=255)
                                                                                                           ggTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGGGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACgg                <  4:112192/135‑1 (MQ=255)
                                                                                                             ttATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGtt              >  5:445770/1‑135 (MQ=255)
                                                                                                              tataGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGtt              >  5:418999/1‑134 (MQ=255)
                                                                                                                taGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTAc            <  1:269630/134‑1 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAc  >  7:208072/1‑135 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAc  <  7:178272/135‑1 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAc  >  5:424938/1‑135 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAc  <  5:39861/135‑1 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAc  >  3:404225/1‑135 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAc  >  3:262296/1‑135 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAc  >  3:223274/1‑135 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGCTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAc  >  4:181652/1‑135 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCACTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCTGTCTGCACGGTTACATTCTGGTAc  >  3:20015/1‑135 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGGCCGGCTCGATGACCATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgctggcggctgg                                          <  5:331481/95‑1 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGGCCGGCTCGATGACAATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCgctggcggctgg                                          >  6:331481/1‑95 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGTCGCCCGGCTCCATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAc  >  5:180248/1‑135 (MQ=255)
                                                                                                                         acaTACACCACTTGCGGGGCGGCCGGCGCGAGGACGATGGTGGGGGGCGGGCTGCTGGCCGCTGCCCCTTGGGGGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAc  <  5:266050/135‑1 (MQ=255)
                                                                                                                             |                                                                                                                                  
GACAATCGCCACGCCGGTACCGAAGGCCAGGCCGGCCACCAGCGCGGAACCGGGGTAGTACATGGGCGGCGAGGCCGGGTAGGCCCAGGTGCCATAGGTGGTGGTCGGGTTATAGCTGGGCACATACACCACTTGCGGGTCGGCCGGCTCGATGACGATGGTGGTGGGCGGGCTGCTGGCCGCTGCCCCTTGGGTGCTGCTGCCGCTGGCGGCTGGCGCAGGTGCAGGTGCGGTCTGCACGGTTACATTCTGGTAC  >  ACB122/3985964‑3986219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑