Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	4,111,471	A→C	16.5%	T172T (ACA→ACC)	iorB_2 →	Isoquinoline 1‑oxidoreductase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	4,111,471	0	A	C	16.5%	110.5 / 2.8	66	T172T (ACA→ACC)	iorB_2	Isoquinoline 1‑oxidoreductase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (26/27);  new base C (6/7);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCACCGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTG  >  ACB122/4111339‑4111604                                                                                                                                     |                                                                                                                                         cagcACCGGGGGCAGCATGGCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑cc                                                                                                                                       <  6:315275/132‑1 (MQ=255) cATCACCGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑cc                                                                                                                                       <  6:5699/135‑1 (MQ=255) cATCACCGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑cc                                                                                                                                       <  7:236267/135‑1 (MQ=255) cATCACCGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑cc                                                                                                                                       <  5:334896/135‑1 (MQ=255) cATCACCGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑cc                                                                                                                                       <  4:212474/135‑1 (MQ=255)   tCACCGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑cc                                                                                                                                       <  4:2890/133‑1 (MQ=255)   tCACCGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑cc                                                                                                                                       >  3:2890/1‑133 (MQ=255)   tCACCGGGGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCgg                                                                                                                                     <  8:221600/135‑1 (MQ=255)      ccGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTa                                                                                                                                  <  4:263891/135‑1 (MQ=255)      ccGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTa                                                                                                                                  <  5:188100/135‑1 (MQ=255)      ccGGGGGCAGCAGGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTa                                                                                                                                  <  2:206434/135‑1 (MQ=255)       cGGTGGCAGCATGTCGGTGCGCATGTGCTACCCCACCATGCGCCGTCTTGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGGACCGGTCGATGAGCTTTCTACCACA‑CCGGGTaa                                                                                                                                 <  4:23602/135‑1 (MQ=255)       cGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCCCC‑CCGGGTaa                                                                                                                                 >  7:163137/1‑135 (MQ=255)       cGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTaa                                                                                                                                 <  6:9004/135‑1 (MQ=255)       cGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCGTTAGAACAAA‑CCgggg                                                                                                                                   >  3:250118/1‑132 (MQ=255)       cGGTGGCAGCATGTCGGTGAGCATGAGCTACGCCACGATGAGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCATGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTaa                                                                                                                                 <  3:70050/135‑1 (MQ=255)        ggTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACC‑CCGGGTAAc                                                                                                                                >  3:362929/1‑135 (MQ=255)        ggTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCCGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGACGATGAGCTTTGAACCACA‑CCGGGGAAc                                                                                                                                >  5:169852/1‑135 (MQ=255)           ggCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑gta                                                                                                                            <  7:141780/135‑1 (MQ=255)            gcagcaTGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCGACCACA‑CCGGGTAAC‑gta                                                                                                                            >  7:329333/1‑134 (MQ=255)            gcagcaTGTAGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑cc                                                                                                                                       >  1:104432/1‑124 (MQ=255)            gcagcaTGTAGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑cc                                                                                                                                       <  2:104432/124‑1 (MQ=255)                 aTGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGCCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACC‑CCGGGTAAC‑GTAGTACAt                                                                                                                      >  1:250867/1‑135 (MQ=255)                     cGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATAcc                                                                                                                   <  5:90727/134‑1 (MQ=255)                         gcgcATGAGCTACCCCACCATGCGCGGTCTGGGCGCGCGCGCCCGGGGCATGCTGCTGCTGGCGGGCGCCGAACACCTGGGTGTCCCGGGCGATGAGCTTTCTACCACA‑CCGTGAAAC‑GTAGTACAAACCACGAc                                                                                                              <  2:60699/135‑1 (MQ=255)                            cATGAGGTACCCCACCATGCGCGGGCTGGGCGCGCTCGCCCGGGGCATGGTGCGGCAGGCGGGCGCCGAACACCGGGGTGAACGGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCgg                                                                                                           <  7:349447/135‑1 (MQ=255)                             aTGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACC‑CCCGGTAAC‑GTAGTACATACCCCGACCggg                                                                                                          >  4:378034/1‑134 (MQ=255)                               gAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATTCTCGTTCTACCCCA‑CCGGGTAAC‑GTAGTCCCGAACACGCCCGGAcc                                                                                                        >  8:357837/1‑134 (MQ=255)                                acccaCCCCACCATGCGCCGGCTGGGCGCGCTCGCCCGGGGCATGCTGCGGCAGGCGGGCGCCGAACCCGTGGGTGTCCCGGGCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGAc                                                                                                         <  8:398393/129‑1 (MQ=255)                                             tGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTAACACA‑CCGGGTAAC‑GTAGTACAATACACGCCCGGACGGTCCCTACCCGACg                                                                                          >  2:138332/1‑135 (MQ=255)                                             tGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTATAACCCA‑CCGCGTAAC‑GTAATACAAACCACGAACGGACGTTCCGTACCCAACg                                                                                          >  8:300290/1‑135 (MQ=255)                                              gcgcCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGAGGGGCGTTCTACCACA‑CCGGGTAAC‑GTAGTAAATACCACGACCGGCCCTTCCCTACCCTACgg                                                                                         >  1:105965/1‑135 (MQ=255)                                                gcCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTATACCACACCCGGTTAAC‑TTA‑TACATACCACGACCGGCCGTTCCGTACCCTACgg                                                                                         >  5:86352/1‑133 (MQ=255)                                                  cGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑cc                                                                                                                                       >  7:159953/1‑86 (MQ=255)                                                  cGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑cc                                                                                                                                       <  8:159953/86‑1 (MQ=255)                                                  cGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGAc                                                                                                         <  5:150546/115‑1 (MQ=255)                                                  cGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGAc                                                                                                         >  6:150546/1‑115 (MQ=255)                                                   gTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCAACCACC‑CCGGGTAAC‑CTAGTACATACCCCGCCCGGCCGTTCCGTACCCTACGGTgc                                                                                      >  7:337158/1‑132 (MQ=255)                                                       gggCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTATTACCTACCACGCCCGGACGTTCCGTACCCTACGGTGAGCTGG‑c                                                                               >  5:236239/1‑135 (MQ=255)                                                       gggCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCGTTCTACCACA‑CCGGGTAAC‑GTAGTACAGACCACGACCGGACGTTCCGTACCCGACGGTGAGCTGG‑c                                                                               >  7:352016/1‑135 (MQ=255)                                                          cgcgcgCCCCCGGGGCATGCTGCTGCAGGGGGGCCCCGAACACCTGGGTGTACCGGTCGATGAGGTTTCTACCACA‑CCGGGTAAC‑GTAGAAAATCCCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCgg                                                                            <  7:134114/135‑1 (MQ=255)                                                               gcGCCGGGGGCAGGCGGCGGCAGCGGGGCGCCCAACACCTGGGTGAACCGGGCGAGGAGCTTTCTCCCACA‑CCGGGAAAC‑GTAGAACAACCCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTcgcg                                                                       <  2:250867/134‑1 (MQ=255)                                                                 gCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCgcgc                                                                      >  8:80181/1‑134 (MQ=255)                                                                 gCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCCGGcgcggc                                                                     >  6:206437/1‑133 (MQ=255)                                                                    gggggCATGCGGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATg                                                                  <  2:358700/134‑1 (MQ=255)                                                                            gctgctgcAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTAAGGTGAGCTGG‑CCGGTCGCGCCATGGCCCTGcc                                                          >  3:377936/1‑135 (MQ=255)                                                                              tgctgcAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCCCA‑CCGGGTAAC‑GGAGTACATACCCCGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCgg                                                        >  2:250184/1‑135 (MQ=255)                                                                              tgctgcAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACC‑CCGGGTAAC‑GTAGTACATACCCCGCCCGGCCGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCCTGGAccggccgg                                                        >  8:428382/1‑135 (MQ=255)                                                                              tgctgcAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCgg                                                        >  2:283429/1‑135 (MQ=255)                                                                              tgctgcAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTAACACA‑CCGGGTAAA‑GTAGTACATACCACCACCGGACGTTCCGTACCCTACCGTGAGCTGG‑CCCGTCGCGCCCAGGACCTGcccg                                                        >  8:200389/1‑133 (MQ=255)                                                                              tgcggcAGGCGGGCGCCGAACCCGTGGGTGTCCGGGTCGATGCGCTTGGTACCACC‑CCGGGAAAG‑GTAGTACATACCACGACCGGACGTTCCGTAGCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCgg                                                        <  6:313778/135‑1 (MQ=255)                                                                                    aGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGCGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCg                                                  >  1:286255/1‑135 (MQ=255)                                                                                    aGGCGGGCGCCGAACACCTGGGTGTACCGGTCGAGGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGCCCGGACGTTCCGTACCCGACGGTGAGCTGG‑CCGGTCGCGCCAAGGACCGGCCGGTGCCCg                                                  >  5:313778/1‑135 (MQ=255)                                                                                        gggcgCCTAACACGTGGGTGTCGGGGTCCATGCGCTTTCACCCCCC‑CCGGGTACC‑GTCGTACAAACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGAccc                                              <  3:450319/135‑1 (MQ=255)                                                                                        gggcgCCGAACCCCGGGGTGTCCCGGGCGAGGCGCTTTCTACCCCC‑CCGGGTAAC‑GTAGAACATCCCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGAccc                                              <  6:340753/135‑1 (MQ=255)                                                                                        gggcgCCGAACACGTGGGTGAACGGGTCGATCCGCTTTCACCCACA‑CCGGGAAAC‑GTATTAAATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGAccc                                              <  6:86352/135‑1 (MQ=255)                                                                                        gggcgCCGAACACCTGGGTGCACCGGGCGATGCGCTTCCTACCCCC‑CCGGCTACC‑GTAGTACATCCCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGAccc                                              <  7:298237/135‑1 (MQ=255)                                                                                        gggcgCCGAACACCTGGGTGAACCGGTCGATGAGCTTGCTACCACC‑CCGGGTACC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGAccc                                              <  4:149023/135‑1 (MQ=255)                                                                                        gggcgCCCACCCCCTGGGTGTCCCGGCCGATCAGCTTTCACCCACA‑CCGGGTAAC‑GTCGAACAACCCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGAccc                                              <  5:206437/135‑1 (MQ=255)                                                                                           cgcCGAACACCTGGGTGTACCGGTCGATGAGGTTTCTACCACA‑CCGGGGTAC‑GTAGTAAATACCACGACCGGACCTTACCTACCCGACGGTGAGCAGGCCCGGAGCCCCCAGGGACATGCCGGGGCCCCCCCCgc                                            >  6:279570/1‑134 (MQ=255)                                                                                             ccGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCa                                         >  4:110395/1‑135 (MQ=255)                                                                                                       gggTGTCCGGGTCGTTGTGCTTTCT‑CCCCC‑CCGTGTTACGGTATAAAATCCCACGACCGGAGGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCt                               <  1:111939/135‑1 (MQ=255)                                                                                                       gggTGTCCCGGTCGTGGCGCTTGCTACCCCC‑GCGGGAAAC‑GTAGAACATCCCACGACCGGACGTTCCGTCCCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCt                               <  6:433431/135‑1 (MQ=255)                                                                                                                      tGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTTG‑CCCGTCGCGCCCATGCCCGTCCCGTGCCCGCCCCCGCCCCCGTCAACCCGCGCGCCCccacccc                >  4:441385/1‑134 (MQ=255)                                                                                                                          cTTTCAACCACA‑CCGGGAAAG‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGtt             <  3:165368/134‑1 (MQ=255)                                                                                                                               tACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGCCCCGGCCAGCGTCAAGCAGCGCGCCCCCAGCCAGTTTCGCTg       >  2:121399/1‑135 (MQ=255)                                                                                                                                   aca‑cCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGAtt   >  8:421889/1‑135 (MQ=255)                                                                                                                                    ca‑cCGGGAAAC‑GTTCTACACACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTg  <  7:428382/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CATCACCGGTGGCAGCATGTCGGTGCGCATGAGCTACCCCACCATGCGCCGTCTGGGCGCGCTCGCCCGGGGCATGCTGCTGCAGGCGGGCGCCGAACACCTGGGTGTACCGGTCGATGAGCTTTCTACCACA‑CCGGGTAAC‑GTAGTACATACCACGACCGGACGTTCCGTACCCTACGGTGAGCTGG‑CCGGTCGCGCCATGGACCTGCCGGTGCCCGACCCGGCCAGCGTCAAGCTGCGCGACCCCAGCCAGTTTCGCTGGATTG  >  ACB122/4111339‑4111604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑