| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,135,592 | A→C | 24.3% | N27T (AAC→ACC) | tgnC → | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,135,592 | 0 | A | C | 24.3% | 100.1 / 3.6 | 61 | N27T (AAC→ACC) | tgnC | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (22/22); new base C (3/14); total (25/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAA > ACB122/4135458‑4135724 | tgGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCaa > 3:229113/1‑135 (MQ=255) ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGCCCTTCGACAGCCTCAACCCgg > 7:55339/1‑135 (MQ=255) ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 5:133758/135‑1 (MQ=255) ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 3:233630/135‑1 (MQ=255) ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 2:311264/135‑1 (MQ=255) ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 6:49928/135‑1 (MQ=255) cACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCAcc > 1:151982/1‑135 (MQ=255) cACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc > 4:451613/1‑135 (MQ=255) cACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACAGCCAATGGCGCGATGCCCAGAGCGGCAAGCCCTTCGACAGCCTCAACCCGGCCAcc > 2:148946/1‑135 (MQ=255) cccAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc < 4:40876/133‑1 (MQ=255) cccAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc < 3:333216/133‑1 (MQ=255) acgCCCGCACCCTCCCCGAATCCGCCACGGGGATTGTTGCATGACCCGCGGGCGTTGCCAGATGTGCATCGACGGCCAAGGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa < 4:462334/133‑1 (MQ=255) aaGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCAGCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa < 7:389927/135‑1 (MQ=255) gCCCGCACCCTCACCGAATCCGCCAAGTGGATTGTTGCATGACGCTCGTTCGTTTCCAGATGTGCATCCACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCAACCGCCCAgg < 6:129235/135‑1 (MQ=255) cccGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCAGGCCACCGCCCAGGc > 2:166346/1‑135 (MQ=255) cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGCCAGCCTCACCCCGGCCCCCGCCCCGGccg > 1:366792/1‑134 (MQ=255) cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCt > 2:345488/1‑135 (MQ=255) ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGgc > 4:109068/1‑134 (MQ=255) cACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCGGGgcgcc > 8:112346/1‑133 (MQ=255) ccGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCGGGGCGCCAcg > 5:165529/1‑134 (MQ=255) gAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 1:330368/108‑1 (MQ=255) gAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg > 2:330368/1‑108 (MQ=255) gAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCaccagc > 3:328606/1‑130 (MQ=255) aaTCGGCCAAGGGGATTGTTGCATGACCGTGGTTGTTTTCCAGATGTGAAACGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCGGGGGCGCAACTGcc < 8:246271/135‑1 (MQ=255) tCCGCCACGGGGTTTTTTGAATGACCGTCGTGCGTTTCCAGTTGTGCAGCGACGGCCAATGGGGCGATCCCCAGAGCGCCAAGACCTTCGACAGCCACACCCCGCCCCCCGCCCAGGCCGGGGCGCAACTGCCCg < 4:229113/135‑1 (MQ=255) gccGCCCAGGGGATTGTTGCATGACCCTCGTGCGTTGCCAGATGGGCATCGACGGCCAATGGCGCGAGGCCCAGAGCGCCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCg < 3:324778/134‑1 (MQ=255) ccAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCAGCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGc < 4:36011/135‑1 (MQ=255) cAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCgccgcc > 3:310725/1‑135 (MQ=255) cggggATTGTTGCTGGCCCCTCGTTCGTTTCCAGATGTGCATCGCCGCCCACTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGa < 8:327020/134‑1 (MQ=255) aTTGTTGCATGACCCTCGTTCTTTTCCTCATGTGCATCTACGGCCTAGGGGGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTAAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAg < 4:283858/135‑1 (MQ=255) gttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCCACTGCCCGCCGCCGCTGAAGCCg > 5:152978/1‑135 (MQ=255) ttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa > 8:183678/1‑99 (MQ=255) ttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa < 7:183678/99‑1 (MQ=255) cATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGAtgt > 4:40792/1‑135 (MQ=255) gACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCCCGCCAGGGCGCAACAGCCCGACGCCGAGGAACCCGCAGTGGa > 8:290341/1‑135 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAg < 8:450377/134‑1 (MQ=255) tgcccgATGTGCAGCGCCGGCCACTGGCGCGAGGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAg < 8:159193/130‑1 (MQ=255) ccctttgtcCATCGCCGGCCAATGGCGCGAGGCCAAGAGCGCCAAGACCTTCGACCGCCCCACCCCGCCCACCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAgg < 3:109068/129‑1 (MQ=255) cAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCAGGCCAGGGCGCAACTGCCCCCCGCCGAATAAGACGAATTGGAGCTGGCCGGTAAGgcc > 7:408462/1‑135 (MQ=255) cATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg > 1:165781/1‑59 (MQ=255) cATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 2:165781/59‑1 (MQ=255) cATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGGCCCTGCCCGCCGCCGCGGGAGCCGCGGTGGGGCTGGCCGGTGAGGCCGccccccg > 7:271907/1‑131 (MQ=255) aTCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACGGCCCGCCGCCGAGGCAGCCGCTGTGGAGCTGGCCGGTCAGGCCGCCCCGCGt > 1:86476/1‑135 (MQ=255) aTCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCACGCCGCCCAGCGt > 7:155546/1‑135 (MQ=255) cgccgcccACTGGCGCGATGCCCCGAGCGGCACCCCCTCCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGc < 5:238837/129‑1 (MQ=255) aCGCCCACTGGCGCGAGGCCCCGAGCGGAACGCCCTTCGACCGCCTCACCCCGCCCCCCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGc < 2:172302/133‑1 (MQ=255) cGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCCCCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGGCCGGTCAGGCCGCCCACCGGGccgt > 2:203105/1‑133 (MQ=255) aaTGGCGCGAGGCCCAGAGCGGCAAGACCTGCGACAGCCTCAACCCGGCCCCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa < 6:464864/135‑1 (MQ=255) cgcgATGCCCAGAGCGGCAAGACCTTCGACAGCCTCCACCCGGCCACCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAg < 7:328316/135‑1 (MQ=255) ggCCCAGCGCGGCAAGACCTTCGACAGCCGCACCCCGGCCCCCCCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 6:192886/134‑1 (MQ=255) cAGCGCCGCAACACCTTCGACAGCCTCACCCCGCCCCCCGCCCCGGCGGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGgcgc < 8:60432/135‑1 (MQ=255) cAGAGCGGCAAGACCTGCGACAGCCTCACCCCGGCCACCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGGGCCTTCGACAGCAAGGCGTGgcgc < 6:170843/135‑1 (MQ=255) cAGAGCGGCAAGACCTCCGACAGCCTCACCCCGGCCACCGCCCTGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGgcgc < 6:163055/135‑1 (MQ=255) cGGCAAGACCTGCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCAt < 4:328606/135‑1 (MQ=255) agcccTTCGACAGCCCCACCCCGGCCCCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCg < 2:151982/132‑1 (MQ=255) ttCGACCGCCTCACCCCGCCCACCCCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAcc < 1:82660/135‑1 (MQ=255) ttCGACAGCCGCACCCCGGCCACCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAcc < 2:40996/135‑1 (MQ=255) gCCTCACCCCGGCCCCCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 5:287772/135‑1 (MQ=255) gCCTCACCCCGGCCCCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 1:148946/135‑1 (MQ=255) cccccAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 2:150318/131‑1 (MQ=255) cAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGCTGTGGAGCTGGCCGTTGAGGCCCCCCCGCGTGCCGTCGCCCGCAAGGCGTGGCGCAGCATCCCCGCGACCGCCCCCGGCaa > 8:189562/1‑135 (MQ=255) cAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCaa > 4:321367/1‑135 (MQ=255) | TGGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAA > ACB122/4135458‑4135724 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |