Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	397,442	G→A	5.2%	D555D (GAC→GAT)	CJ019_00365 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	397,442	0	G	A	5.2%	259.3 / 3.1	78	D555D (GAC→GAT)	CJ019_00365	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (40/33);  new base A (2/2);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

AACGGTGCACAGGCCAGGCCTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCTGGTGGCCAAGGCTGTCGTTGAT  >  ACB122/397320‑397576                                                                                                                           |                                                                                                                                       aaCGGTGCACAGGCCAGGCCTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGagcagc                                                                                                                            >  1:336995/1‑135 (MQ=255)       gctcagggcagtcCTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGTGTGCACAACAACTGTTGAGCAACTCCTTGGCCATCTTCAAGGCTGGTGTCGTCTAGCAGCACGGCg                                                                                                                      <  7:218542/132‑1 (MQ=255)       gCACAGGCCAGGCCTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCg                                                                                                                      <  2:263784/135‑1 (MQ=255)       gCACAGGCCAGGCCTACCGAGGCGGGGACGTTGATCAGCTGCTTGTCGACGAACATCGGCTTGTCGAGGGGGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCg                                                                                                                      <  7:7321/135‑1 (MQ=255)         acaGGCCAGGCCTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGaa                                                                                                                    >  6:446120/1‑135 (MQ=255)         acaGGCCAGGCCTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGaa                                                                                                                    >  7:251463/1‑135 (MQ=255)           aggccaggccTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCGTCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACt                                                                                                                  >  3:337880/1‑135 (MQ=255)           aggccaggccTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACt                                                                                                                  >  3:199879/1‑135 (MQ=255)              ccaggccTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCg                                                                                                                >  5:177043/1‑134 (MQ=255)                aggccTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTa                                                                                                             >  3:81422/1‑135 (MQ=255)                   ccTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTg                                                                                                          >  8:73972/1‑135 (MQ=255)                   ccTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTAGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCCGCACGGCGAACTCGTTACTg                                                                                                          >  2:206003/1‑135 (MQ=255)                     tACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGCGCAGCACGGCGAACTCCTTACTgg                                                                                                         >  3:6659/1‑134 (MQ=255)                     tACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTgg                                                                                                         >  5:311833/1‑134 (MQ=255)                     tACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTgg                                                                                                         >  1:339168/1‑134 (MQ=255)                     tACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCGTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTgg                                                                                                         >  2:376812/1‑134 (MQ=255)                          aGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGCACTCGTTACTGGCAAAgc                                                                                                   >  4:98772/1‑135 (MQ=255)                          aGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgc                                                                                                   >  7:379494/1‑135 (MQ=255)                          aGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgc                                                                                                   >  5:177804/1‑135 (MQ=255)                          aGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgc                                                                                                   >  6:315900/1‑135 (MQ=255)                          aGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgc                                                                                                   >  6:340007/1‑135 (MQ=255)                          aGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgc                                                                                                   >  6:407370/1‑135 (MQ=255)                          aGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgc                                                                                                   >  6:56423/1‑135 (MQ=255)                          aGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgc                                                                                                   >  2:190236/1‑135 (MQ=255)                          aGGCGCTGACGTTGATCAGCTGGTTGTCGACCAACAACGGCTTGTCGAGCGTGCACAACAACTGTTGAGCAACGCCTAGGCCATCGTCAAGTCTGCTGTCGTCGAGCAGCACGGCGAACACGTTACTGGCAAAgc                                                                                                   >  5:40614/1‑135 (MQ=255)                              ggTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgcgcgc                                                                                               <  4:199879/135‑1 (MQ=255)                              ggTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgcgcgc                                                                                               <  6:123894/135‑1 (MQ=255)                               gTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgcgcg                                                                                                >  8:134097/1‑133 (MQ=255)                               gTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgcgcg                                                                                                >  7:435132/1‑133 (MQ=255)                                tGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAgcgcgc                                                                                               <  8:435132/133‑1 (MQ=255)                                  aCGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAgg                                                                                           >  4:196941/1‑135 (MQ=255)                                   cGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGa                                                                                          >  7:33573/1‑135 (MQ=255)                                        aTCAGGTGGTTGGCGACGAACATCGGGTTGTCGCTGGTGCAAAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGGCGGCGAGCAGCACGGCTAACTCGTTACTGGCAACGCGCGCCAGGATGCCa                                                                                     <  1:206003/135‑1 (MQ=255)                                        aTCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTCCACAACAACTGTTGAGCAACGCCTTGGCCAGCTTCACGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAACGCGCGCCAGGATGcc                                                                                      <  5:453523/134‑1 (MQ=255)                                        aTCAGCTGGTAGTCGACGAACAGCGGCTTGTCGCGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCAGCTTCAAGGCTGGGGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGcc                                                                                      <  2:153253/134‑1 (MQ=255)                                        aTCAGCGGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCACGGCTGGTGTGGTCGCGCAGCACGGCGAACTCGTTACTGGCAACGCGCGCCAGGATGCCa                                                                                     <  7:207951/135‑1 (MQ=255)                                         tCAGGTGGTTGTCGACGTACTTCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCGATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCa                                                                                     <  8:144426/134‑1 (MQ=255)                                         tCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGTGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGGTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCAc                                                                                    <  1:263517/135‑1 (MQ=255)                                         tCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCa                                                                                     <  8:58194/134‑1 (MQ=255)                                         tCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCa                                                                                     <  8:207371/134‑1 (MQ=255)                                         tCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCAc                                                                                    <  5:446120/135‑1 (MQ=255)                                                 ttGTCGCCGAACATCGGCTTGTCGAGGGTGCACAACAACTGGTGAGCAACGCCTTGGCCATCTTCACGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGc                                                                            <  6:14667/135‑1 (MQ=255)                                                 ttGTCGACGAACATCGTCTTGTCGAGGGTGCACAACAACTGTGGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGc                                                                            <  3:284867/135‑1 (MQ=255)                                                     cgacgaACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGc                                                                        <  6:358678/135‑1 (MQ=255)                                                                 gCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCaaa                                                             <  4:81422/134‑1 (MQ=255)                                                                         aGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGcc                                                    <  2:336995/135‑1 (MQ=255)                                                                         aGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTATCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTgg                                                      >  4:340503/1‑133 (MQ=255)                                                                         aGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTATCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTgg                                                      >  4:340499/1‑133 (MQ=255)                                                                              gCACAACAACTGGTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGGCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCt                                               <  8:232903/135‑1 (MQ=255)                                                                                 caacaaCTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGAt                                            <  2:434464/135‑1 (MQ=255)                                                                                  aacaacTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGa                                             >  1:700/1‑133 (MQ=255)                                                                                      acTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATcagca                                       <  6:447656/135‑1 (MQ=255)                                                                                       cTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCa                                                                                     >  7:435850/1‑88 (MQ=255)                                                                                       cTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCa                                                                                     <  8:435850/88‑1 (MQ=255)                                                                                       cTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATcagcag                                      >  7:109790/1‑135 (MQ=255)                                                                                          ttGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCtt                                   >  3:74180/1‑135 (MQ=255)                                                                                          ttGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCtt                                   >  8:37821/1‑135 (MQ=255)                                                                                          ttGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCtt                                   >  1:457984/1‑135 (MQ=255)                                                                                          ttGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCtt                                   >  5:450029/1‑135 (MQ=255)                                                                                            gAGCAACGCCTTGGCCATCTTCAAGGCTGGTTTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTg                                  <  6:413836/134‑1 (MQ=255)                                                                                             aGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGt                                 <  6:450029/134‑1 (MQ=255)                                                                                             aGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGt                                 <  3:190770/134‑1 (MQ=255)                                                                                             aGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTc                                >  3:47735/1‑135 (MQ=255)                                                                                             aGCAACGCCTTGGCCATCTTCAAGGCTGGTATCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCTCCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGt                                 <  3:340503/134‑1 (MQ=255)                                                                                             aGCAACGCCTTGGCCATCTTCAAGGCTGGTATCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGt                                 <  3:340499/134‑1 (MQ=255)                                                                                              gCAAGGCGTTGGCCATCTTCAAGGGTGGTGGCGTCGAGCTGCACGGCTAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCg                               <  6:283973/135‑1 (MQ=255)                                                                                                  cGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCgg                           >  7:420310/1‑135 (MQ=255)                                                                                                   gCCTTGGCCATCTTCAAGGCGGGGGGCGTCGAGCAGAACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGt                          <  1:376812/135‑1 (MQ=255)                                                                                                     cTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGATCTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCGGATCAGCAGCTTGTCGCCGGTCt                        >  2:418021/1‑135 (MQ=255)                                                                                                       tGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCtgg                      >  7:281727/1‑135 (MQ=255)                                                                                                          ccATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCtggtgg                   <  4:175200/135‑1 (MQ=255)                                                                                                          ccATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCtggtg                    <  7:134097/134‑1 (MQ=255)                                                                                                                 cAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCTGGTGGCCAAGGc            >  7:57739/1‑135 (MQ=255)                                                                                                                 cAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCTGGTGGCCAAGGc            >  7:342181/1‑135 (MQ=255)                                                                                                                 cAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCTGGTGGCCAAGGc            >  5:141870/1‑135 (MQ=255)                                                                                                                       tGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCTGGTGGCCAAGGCTGTCGt      <  8:251463/135‑1 (MQ=255)                                                                                                                       tGGGGTCGGCGAGCAGCACGGCTAACTCGTAACTGGCAACGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCTGGTGGCCAAGGCTGTCGt      <  6:311833/135‑1 (MQ=255)                                                                                                                       gggTGTCGGCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGAGGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCTGGTGGCCAAGGCTGTCGt      <  4:6659/134‑1 (MQ=255)                                                                                                                           gtcgtcGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCTGGTGGCCAAGGCTGTCGTTGag  >  2:167824/1‑134 (MQ=255)                                                                                                                           |                                                                                                                                       AACGGTGCACAGGCCAGGCCTACCGAGGCGGTGACGTTGATCAGCTGGTTGTCGACGAACATCGGCTTGTCGAGGGTGCACAACAACTGTTGAGCAACGCCTTGGCCATCTTCAAGGCTGGTGTCGTCGAGCAGCACGGCGAACTCGTTACTGGCAAAGCGCGCCAGGATGCCACCGCTGTGCAGGCTGTTGCGCAAACGCCTGGCCAGGCTGATCAGCAGCTTGTCGCCGGTCTGGTGGCCAAGGCTGTCGTTGAT  >  ACB122/397320‑397576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑