Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	4,151,792	G→A	5.3%	G53S (GGT→AGT)	CJ019_03909 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	4,151,792	0	G	A	5.3%	252.2 / 2.9	75	G53S (GGT→AGT)	CJ019_03909	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (41/30);  new base A (2/2);  total (43/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

CGCCTTTGCCCCTACCCTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTACGCATTGCTGTGGCATTTTTTGGCCTGCGGGTAAGCCT  >  ACB122/4151659‑4151925                                                                                                                                      |                                                                                                                                      cGCCTTTGCCCCTACCCTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCCTCAGCCCGCTGAGCGTgg                                                                                                                                       >  1:422743/1‑134 (MQ=255) cGCCTTTGCCCCTACCCTCTCCACCCGAGGGCGGCTCAATGGGATTCTATTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCAAACCTGGTCATCAGCCCGCTGATCGTgg                                                                                                                                       >  6:220824/1‑134 (MQ=255)          cccTACCCTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTAtcgtcg                                                                                                                              <  7:362721/134‑1 (MQ=255)          cccTACCCTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTAtcgtcg                                                                                                                              <  7:8485/134‑1 (MQ=255)          cccTACCCTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGc                                                                                                                             <  4:277373/135‑1 (MQ=255)          cccTACCCTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGGCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTAtcgtcg                                                                                                                              <  2:325930/134‑1 (MQ=255)            cTACCCTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAg                                                                                                                           <  4:164191/135‑1 (MQ=255)                ccTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGt                                                                                                                         <  7:164468/133‑1 (MQ=255)                ccTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGt                                                                                                                         <  6:40488/133‑1 (MQ=255)                ccTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGt                                                                                                                         <  7:75586/133‑1 (MQ=255)                ccTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGc                                                                                                                       >  1:209404/1‑135 (MQ=255)                 ctctCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGcc                                                                                                                      >  4:71867/1‑135 (MQ=255)                 ctctCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGcc                                                                                                                      >  3:226643/1‑135 (MQ=255)                     ccACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCtt                                                                                                                    <  2:243319/133‑1 (MQ=255)                      cACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGACACCCTGGGCATCAGCCCGCTGATCGTGGTTATCGTCGCAGGTGCCTTgga                                                                                                                 >  4:372894/1‑133 (MQ=255)                       ccccGAGGGCGGCTCAATGGGATTCTCTTCATCGCGCTGTTCGCGGTCGCAGTCCCGCAACTGGCGGCCAAGCCCGCTATCGCCACCCTGGGCATCAGCCCGCTGATCGTGGCTTTCGTCGCACGGGCCTAGTAt                                                                                                                >  2:207060/2‑135 (MQ=255)                       ccccGAGGGCGGCTCAATGGGATTATCTTCGTCGCGCTGTTCGAGGTCGCAGTCACGCAACTGGCCCCCATGCCCGCAATCGACACCCTGGGCATCAGCACGCTGATGGTGGGTAACGTCGCAGGTGGCTTGTAt                                                                                                                >  4:199450/2‑135 (MQ=255)                       aCCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGCTCGTGGGTATCGTCGCAGGTGCCTTGTAt                                                                                                                >  4:90238/1‑135 (MQ=255)                       aCCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTAt                                                                                                                >  3:202324/1‑135 (MQ=255)                       aCCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTAt                                                                                                                >  4:347287/1‑135 (MQ=255)                       aCCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTAt                                                                                                                >  7:430223/1‑135 (MQ=255)                       aCCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTAt                                                                                                                >  6:318509/1‑135 (MQ=255)                       aCCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCGTGTAt                                                                                                                >  6:5196/1‑135 (MQ=255)                       aCCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGAGTATCGTCGCAGGTGCCTTGTAt                                                                                                                >  5:321420/1‑135 (MQ=255)                       aCCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGAGTATCGTCGCAGGTGCCTTGTAt                                                                                                                >  5:315374/1‑135 (MQ=255)                       aCCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGAGCGGGGGTATCGTCGCTGGTGCGTTGTAt                                                                                                                >  1:462317/1‑135 (MQ=255)                            aGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAg                                                                                                                           <  7:23508/119‑1 (MQ=255)                            aGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAg                                                                                                                           >  8:23508/1‑119 (MQ=255)                            aGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCaa                                                                                                           >  3:422513/1‑135 (MQ=255)                             gggCGGCTCAATGGGCTTCTCGTCGTCGCGCTGGTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAAc                                                                                                          <  6:311493/135‑1 (MQ=255)                             gggCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCACCCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAAc                                                                                                          >  6:82240/1‑135 (MQ=255)                             gggCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAAc                                                                                                          <  7:373241/135‑1 (MQ=255)                             gggCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAAc                                                                                                          <  8:232628/135‑1 (MQ=255)                             gggCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAAc                                                                                                          <  1:151255/135‑1 (MQ=255)                              ggcggcTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAAcg                                                                                                         >  6:84068/1‑135 (MQ=255)                                   cTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTg                                                                                                    >  1:214058/1‑135 (MQ=255)                                   cTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTg                                                                                                    >  7:443031/1‑135 (MQ=255)                                     cAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCg                                                                                                  >  7:445026/1‑135 (MQ=255)                                      aaTGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCTACTCGCGGCCATGCCCCCTATCGCCAACCTGGGCTTCAGCCCCCTGATCCGGGGTATCGTCGCACGTGCGTTGTATGGCAACGCGCTCCg                                                                                                  >  8:192912/1‑134 (MQ=255)                                      aaTGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGt                                                                                                 >  6:450878/1‑135 (MQ=255)                                       aTGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTg                                                                                                <  2:142532/135‑1 (MQ=255)                                                tctTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTAc                                                                                       >  7:292445/1‑135 (MQ=255)                                                tctTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTAc                                                                                       >  7:85813/1‑135 (MQ=255)                                                     gtcgCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAAc                                                                                                          <  7:121981/111‑1 (MQ=255)                                                     gtcgCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAAc                                                                                                          >  8:121981/1‑111 (MQ=255)                                                     gtcgCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCgg                                                                                    >  3:425387/1‑133 (MQ=255)                                                     gtcgCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGAGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGc                                                                                   <  6:315374/134‑1 (MQ=255)                                                     gtcgCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGAGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGc                                                                                   <  6:321420/134‑1 (MQ=255)                                                        gcgcTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGc                                                                               >  4:158178/1‑135 (MQ=255)                                                             gTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGggc                                                                          >  4:454093/1‑135 (MQ=255)                                                               tCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGggcg                                                                         >  1:279914/1‑134 (MQ=255)                                                               tCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGggc                                                                          <  4:177946/133‑1 (MQ=255)                                                                 gcgGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCggca                                                                      <  2:188462/135‑1 (MQ=255)                                                                 gcgGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCggca                                                                      >  3:395586/1‑135 (MQ=255)                                                                  cgGTCGCAGTCACGCAACTGGCGGCCATGCCCGCGATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGGATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCgtca                                                                      <  8:342727/134‑1 (MQ=255)                                                                                  aCTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGAAGGCATCAATTTTACCGc                                                     >  8:236893/1‑135 (MQ=255)                                                                                    tGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTc                                                   <  7:116945/135‑1 (MQ=255)                                                                                    tGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTc                                                   <  3:347287/135‑1 (MQ=255)                                                                                    tGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTc                                                   <  3:221328/135‑1 (MQ=255)                                                                                      gcggcCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCGGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTcgc                                                 >  1:54934/1‑135 (MQ=255)                                                                                      gcggcCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTcgc                                                 <  6:307374/135‑1 (MQ=255)                                                                                         gcCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGc                                              <  5:84068/135‑1 (MQ=255)                                                                                         gcCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGc                                              <  5:318509/135‑1 (MQ=255)                                                                                          ccATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGcc                                             >  5:123961/1‑135 (MQ=255)                                                                                           cATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCt                                            <  4:425387/135‑1 (MQ=255)                                                                                              gCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGtt                                         >  1:330280/1‑135 (MQ=255)                                                                                                ccGCTATCGCCAACCTTGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGAGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTAc                                       <  3:372894/135‑1 (MQ=255)                                                                                                ccGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTAc                                       >  4:293494/1‑135 (MQ=255)                                                                                                         ccAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTACGCATTGCtg                              <  4:68206/135‑1 (MQ=255)                                                                                                          cAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTACGCATTGCtgt                             <  8:79939/135‑1 (MQ=255)                                                                                                               tGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTACGCATTGCTGTGGCAt                        >  5:352344/1‑135 (MQ=255)                                                                                                                   cATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCCGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTACGCATTGCTGTGGCAttttt                    >  2:382997/1‑135 (MQ=255)                                                                                                                    aTCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTACGCATTGCTGTGGCAtttttt                   >  2:415119/1‑135 (MQ=255)                                                                                                                          ccGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCCGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTACGCATTGCTGTGGCATTTTTTgg                 <  1:382997/131‑1 (MQ=255)                                                                                                                           cGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTACGCATTGCTGTGGCTTTTTTTGGCCTGc            >  5:367325/1‑135 (MQ=255)                                                                                                                           cGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTACGCATTGCTGTGGCATTTTTTGGCCTGc            >  4:58829/1‑135 (MQ=255)                                                                                                                                aTCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCACTTTTTCCGCTCGCGGCCTGTTACGCATTGCTGTGGCATTTTTTGGCCTGCggg         >  5:170309/1‑133 (MQ=255)                                                                                                                                      ggTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTACGCATTGCTGTGGCATTTTTTGGCCTGCGGGTAAGCCt  >  1:383296/1‑134 (MQ=255)                                                                                                                                      |                                                                                                                                      CGCCTTTGCCCCTACCCTCTCCACCCGAGGGCGGCTCAATGGGATTCTCTTCGTCGCGCTGTTCGCGGTCGCAGTCACGCAACTGGCGGCCATGCCCGCTATCGCCAACCTGGGCATCAGCCCGCTGATCGTGGGTATCGTCGCAGGTGCCTTGTATGGCAACGCGCTGCGTGACGGCGTACCGGCAAGCTGGGCGGCAGGCATCAATTTTTCCGCTCGCGGCCTGTTACGCATTGCTGTGGCATTTTTTGGCCTGCGGGTAAGCCT  >  ACB122/4151659‑4151925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑