Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,359,633 C→T 6.1% R10H (CGC→CAC)  CJ019_04105 ← Beta‑peptidyl aminopeptidase BapA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,359,6330CT 6.1% 202.5 / 3.3 65R10H (CGC→CAC) CJ019_04105Beta‑peptidyl aminopeptidase BapA
Reads supporting (aligned to +/- strand):  ref base C (27/34);  new base T (2/2);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01

ACCCCAGTCAGGATGTCGCGGCCGTTGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCAATTAATTGCCGGTCTTGACCGTGTTCCACGCA  >  ACB122/4359501‑4359765
                                                                                                                                    |                                                                                                                                    
aCCCCAGTCAGGATGTCGCGGCCGTTGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGCCCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCgg                                                                                                                                    >  1:335572/1‑135 (MQ=255)
 ccccAGTCAGGATGTCGCGGCCGTTGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCgg                                                                                                                                    <  6:68594/134‑1 (MQ=255)
  cccAGTCAGGATGTCGCGGCCGTTGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGc                                                                                                                                  <  4:155841/135‑1 (MQ=255)
         aGGATGTCGCGGCCGTTGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGc                                                                                                                           >  8:164094/1‑135 (MQ=255)
                cgcgGCCGTTGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATc                                                                                                                     <  8:7676/134‑1 (MQ=255)
                cgcgGCCGTTGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCg                                                                                                                    <  2:78993/135‑1 (MQ=255)
                cgcgGCCGTTGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCg                                                                                                                    <  2:61583/135‑1 (MQ=255)
                   ggCCGTTGGCGGTGCGTCCTCGCACATTTCTGTGCCCGACCCGCACGCCCGGCACATCGGTGATGGCATTGAGGGGCCCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGGGGCGTCCTCgtgg                                                                                                                 >  4:13161/1‑134 (MQ=255)
                   ggCCGTTGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCgtgt                                                                                                                 >  4:253484/1‑135 (MQ=255)
                        ttGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTc                                                                                                            >  4:181337/1‑135 (MQ=255)
                        ttGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTc                                                                                                            >  4:111527/1‑135 (MQ=255)
                              gTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGAtc                                                                                                      <  1:340076/135‑1 (MQ=255)
                                  gTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGAtctct                                                                                                   <  4:123534/134‑1 (MQ=255)
                                  gTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGAtctct                                                                                                   <  4:306171/134‑1 (MQ=255)
                                   tCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCg                                                                                                 <  7:400834/135‑1 (MQ=255)
                                   tCCTCGAACTTTGCTGTGACAGACCAGAACGCCCGGCTCATCGGTTAGTGATTTGTGTGGACCGGGTTGCTTTTTGGGGTTCTTATTATTCAGGTCGCGGGCACGTGGCTTCATCTTGTCTTTCATGATCTCTCg                                                                                                 <  6:460140/135‑1 (MQ=255)
                                        gCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCtt                                                                                            >  1:366076/1‑135 (MQ=255)
                                              tGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTg                                                                                      <  3:253484/135‑1 (MQ=255)
                                                 tgtgACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCCCGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGgtt                                                                                   >  5:172352/1‑135 (MQ=255)
                                                 tgtgACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGgtt                                                                                   >  8:232068/1‑135 (MQ=255)
                                                 tgtgACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGgtt                                                                                   >  8:450456/1‑135 (MQ=255)
                                                 tgtgACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCGTTCATGATCTCTCGCTCTTGTTTTGgtt                                                                                   >  8:452365/1‑135 (MQ=255)
                                                 tgtgACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGCCCGAACTGAATATTCAGGTCGCGGGCACGTGGGTTCAGCGTGTCTTTCCTGATCTCGCGCTCTTGGTATGgtt                                                                                   >  6:404457/1‑135 (MQ=255)
                                                   tgACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGgtttg                                                                                 >  2:102204/1‑135 (MQ=255)
                                                    gaccgaccCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGgtttgt                                                                                <  3:396377/135‑1 (MQ=255)
                                                     accgaccCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGgtttgtt                                                                               <  8:349235/135‑1 (MQ=255)
                                                         accCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGTCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTTTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCAt                                                                           <  7:20924/135‑1 (MQ=255)
                                                          cccGAACGCCCGGGACATGGGTGTTGGCATTGTGGGGAGCGGGTTGCAGTTGGGAGAACTGAATATTGAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATg                                                                          <  3:6578/135‑1 (MQ=255)
                                                             gAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGCCt                                                                       >  3:154338/1‑135 (MQ=255)
                                                               aCGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTgg                                                                     <  1:102204/135‑1 (MQ=255)
                                                                    cGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGTCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGttt                                                                <  1:405645/135‑1 (MQ=255)
                                                                    cGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGttt                                                                <  7:142947/135‑1 (MQ=255)
                                                                      gCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTgg                                                              >  3:269678/1‑135 (MQ=255)
                                                                      gCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTgg                                                              <  3:111527/135‑1 (MQ=255)
                                                                               tGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAatca                                                       >  6:163532/1‑133 (MQ=255)
                                                                               tGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGGTTGTTTCATGAATTGGGTTTGGTCAatca                                                       >  3:80777/1‑133 (MQ=255)
                                                                                    gCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATcagca                                                 >  7:244823/1‑134 (MQ=255)
                                                                                    gCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATcagca                                                 >  3:223145/1‑134 (MQ=255)
                                                                                    gCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATcagca                                                 >  2:418030/1‑134 (MQ=255)
                                                                                     cATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATcagcag                                                <  3:181337/134‑1 (MQ=255)
                                                                                     cATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATcagcag                                                <  5:112613/134‑1 (MQ=255)
                                                                                       ttGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAAc                                             >  3:217748/1‑135 (MQ=255)
                                                                                          aGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCGATCATCAGCAGAACCg                                           <  7:203156/134‑1 (MQ=255)
                                                                                             ggACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTc                                       <  4:280759/135‑1 (MQ=255)
                                                                                                  gggTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCa                                  >  1:264450/1‑135 (MQ=255)
                                                                                                  gggTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGAAGAACAGGGTCTGACa                                  >  4:227923/1‑135 (MQ=255)
                                                                                                      tGCAGTTGGCCGAACTGAATATTCAGGTCGCTGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGttt                                                                <  3:301565/101‑1 (MQ=255)
                                                                                                      tGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGttt                                                                >  4:301565/1‑101 (MQ=255)
                                                                                                      tGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCaatta                              >  4:101206/1‑135 (MQ=255)
                                                                                                       gCAGTTGGCCGAACTGAATATTCAGGTCGTGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTgg                                                              >  1:285962/1‑102 (MQ=255)
                                                                                                       gCAGTTGGCCGAACTGAATATTCAGGTCGTGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTgg                                                              <  2:285962/102‑1 (MQ=255)
                                                                                                       gCAGTTGGCCGAACTGAATATTCAGGTCGTGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTgg                                                              >  1:292721/1‑102 (MQ=255)
                                                                                                       gCAGTTGGCCGAACTGAATATTCAGGTCGTGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTgg                                                              <  2:292721/102‑1 (MQ=255)
                                                                                                        cAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCaattaat                            <  4:401911/135‑1 (MQ=255)
                                                                                                        cAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCaattaa                             <  7:232068/134‑1 (MQ=255)
                                                                                                        cAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCaattaa                             <  5:4616/134‑1 (MQ=255)
                                                                                                        cAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCaattaa                             <  8:158031/134‑1 (MQ=255)
                                                                                                        cAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCT‑CCaattaat                            <  8:54292/134‑1 (MQ=255)
                                                                                                           ttGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCAATTAATTGc                         >  5:420279/1‑135 (MQ=255)
                                                                                                               ccGAACTGAATATTCAGGTCGCGGGGACGTGGCTTCATCGTGTCTTTGATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTGAATCATCAGCAGAACCGGGTCTGCGAATTTATTGCCgg                      <  7:368747/134‑1 (MQ=255)
                                                                                                                cGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCAATTAATTGCCGGTc                    >  4:411082/1‑135 (MQ=255)
                                                                                                                     tGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCAATTAATTGCCGGTCTTGAc               <  8:37118/135‑1 (MQ=255)
                                                                                                                               gggcgcgGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCAATTAATTGCCGGTCTTGACCGTGTTCcac     <  7:128499/132‑1 (MQ=255)
                                                                                                                               ggTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCAATTAATTGCCGGTCTTGACCGTGTTCcac     <  8:452554/135‑1 (MQ=255)
                                                                                                                                gTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCAATTAATTGCCGGTCTTGACCGTGTTCcacg    <  7:450456/135‑1 (MQ=255)
                                                                                                                                gTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCAATTAATTGCCGGTCTTGACCGTGTTCcacg    <  5:163532/135‑1 (MQ=255)
                                                                                                                                  cgcgGGCACGTGGCTTCAACGTGGCTTTCATGATCTCTCGCTCTTGTTTAGGTTAGTTTCATGACTGCGGTTAGGTCAATCATCAGCAGAACCCTCACTGCCAATTAATTTCCGGTCATGACCGTTTTCaacgcc  >  2:206007/1‑134 (MQ=255)
                                                                                                                                    |                                                                                                                                    
ACCCCAGTCAGGATGTCGCGGCCGTTGGCGGTGCGTCCTCGCACATTGCTGTGACCGACCCGAACGCCCGGCACATCGGTGATGGCATTGAGGGGACCGGGTTGCAGTTGGCCGAACTGAATATTCAGGTCGCGGGCACGTGGCTTCATCGTGTCTTTCATGATCTCTCGCTCTTGTTTTGGTTTGTTTCATGACTGGGGTTTGGTCAATCATCAGCAGAACCGGGTCTGCCAATTAATTGCCGGTCTTGACCGTGTTCCACGCA  >  ACB122/4359501‑4359765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑