Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	4,373,169	G→A	7.3%	A412V (GCG→GTG)	CJ019_04121 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	4,373,169	0	G	A	7.3%	168.0 / 3.7	55	A412V (GCG→GTG)	CJ019_04121	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/22);  new base A (2/2);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

TATGCCAGTACCGCGCTCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGAAAACGCCTTCTTCAAACAGCGGCAGGCCATTGGC  >  ACB122/4373036‑4373299                                                                                                                                      |                                                                                                                                   tATGCCAGTACCGCGCTCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgc                                                                                                                                   >  1:218003/1‑135 (MQ=255)    gCCAGTACCGCGCTCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgcccc                                                                                                                                <  4:233852/135‑1 (MQ=255)      cAGTACCGCGCTCGTTCAGGTAGCGGTTCATGCCATTGGCAACGCCCGACATGGGCGTTTCACCCCGCAGGCCCCCTTCGCAGCGTTCTTGCCGCGCCTCCAAATCAGTGTAGGCACACGCGGGCAccgccccgc                                                                                                                              >  5:281207/1‑135 (MQ=255)      cAGTACCGCGCTCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgccccgc                                                                                                                              >  1:242349/1‑135 (MQ=255)      cAGTACCGCGCTCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgccccgc                                                                                                                              >  2:288445/1‑135 (MQ=255)      cAGTACCGCGCTCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgccccgc                                                                                                                              >  8:301812/1‑135 (MQ=255)      cAGTACCGCGCTCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgccccgc                                                                                                                              >  2:227617/1‑135 (MQ=255)            cgcgCTCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGacac                                                                                                                        >  3:458550/1‑135 (MQ=255)            cgcgCTCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGacac                                                                                                                        >  7:74115/1‑135 (MQ=255)            cgcgCTCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTACACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGAGTAGGCACTCGCTGGAACCGCCCCGCCGacac                                                                                                                        >  8:66332/1‑135 (MQ=255)                 tCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgccccgc                                                                                                                              <  7:305630/124‑1 (MQ=255)                 tCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgccccgc                                                                                                                              <  7:312981/124‑1 (MQ=255)                 tCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgccccgc                                                                                                                              >  8:312981/1‑124 (MQ=255)                 tCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgccccgc                                                                                                                              >  8:305630/1‑124 (MQ=255)                 tCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTa                                                                                                                    >  5:263912/1‑134 (MQ=255)                 tCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTa                                                                                                                    >  5:247099/1‑134 (MQ=255)                 tCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTa                                                                                                                    >  7:24434/1‑134 (MQ=255)                 tCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCACCCCGCCGACACCGTa                                                                                                                    >  7:337126/1‑134 (MQ=255)                 tCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCACCCCGCCGACACCGTa                                                                                                                    >  7:337128/1‑134 (MQ=255)                 tCGGTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAATTCAGTGTAGGCACTCGCTGGACCCGCCCCGCCGACACCGTa                                                                                                                    >  7:278433/1‑134 (MQ=255)                     tCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGc                                                                                                               <  3:8176/135‑1 (MQ=255)                     tCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGc                                                                                                               <  6:333340/135‑1 (MQ=255)                     tCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGc                                                                                                               <  1:242552/135‑1 (MQ=255)                     gcAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGGCGACACCGTAATAGc                                                                                                               <  2:240015/134‑1 (MQ=255)                        ggTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCcgc                                                                                                            <  7:25287/135‑1 (MQ=255)                        ggTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCcgc                                                                                                            <  2:218003/135‑1 (MQ=255)                        ggTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCcgc                                                                                                            <  4:225970/135‑1 (MQ=255)                               gTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCGCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCAcc                                                                                                     <  6:58896/135‑1 (MQ=255)                                ttCATGCCAGTGGAAAAGCCCGACATGGGCGTTACACCCCGCAGGCAACCTTCGACGCGTTCTTCCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCAGCCGACACCGTAATATCCGCGAGCACCa                                                                                                    <  5:305022/135‑1 (MQ=255)                                  cATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATa                                                                                                  >  3:43799/1‑135 (MQ=255)                                      ccATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCCCCATAcccc                                                                                              >  3:29408/1‑135 (MQ=255)                                      ccATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATAcccc                                                                                              >  5:308421/1‑135 (MQ=255)                                             cAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGCCACCGTAATAGCCGCGAGCACCATACCCCTTGAACg                                                                                       >  5:85005/1‑135 (MQ=255)                                                   ccGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGc                                                                                 <  8:271475/135‑1 (MQ=255)                                                   ccGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACACCTTGAACGCAAAg                                                                                  >  6:290132/1‑134 (MQ=255)                                                         tGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGAt                                                                           >  6:31877/1‑135 (MQ=255)                                                             cGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCtt                                                                       <  7:315878/135‑1 (MQ=255)                                                              gTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGCCACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCtt                                                                       >  8:153842/1‑134 (MQ=255)                                                              gTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCtt                                                                       >  5:139069/1‑134 (MQ=255)                                                                  cACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACg                                                                  >  1:342797/1‑135 (MQ=255)                                                                     ccAGCCGGCCACCTTCGAAGCGTTGTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCg                                                               <  1:21205/135‑1 (MQ=255)                                                                     ccAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCTAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCTAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCg                                                               <  1:85/135‑1 (MQ=255)                                                                       agcagGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGAt                                                             >  1:81170/1‑135 (MQ=255)                                                                          cggCCCCCTTCGAAGCGGTCTTGCCGCGGCCCGAAATCAGTGTCGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATCCCCGCGAGCACCATACCCCTTGAACCCCAAGCAACGATTCTTAAACGGCGATGGa                                                          <  5:303174/134‑1 (MQ=255)                                                                          agcccACCTTCGACGCGTGCTTGCAGCGGCTCGAAATCTGTGTAGGCACTCGCTGTAACCGCCCCGCCGACACCGTAATACCCGCGAGCACCATACCCCTTGAACGCCAACCAACGATTCTTAAACGGCGATGGa                                                          <  1:227617/132‑1 (MQ=255)                                                                          agGCCCCCTTCGAAGCGTCCTTGCAGCGGCTCGAAATCAGTGTCGGCACGCGCGGGAACCGCCCCGCCGACACCGTAATCGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGa                                                          <  1:61535/135‑1 (MQ=255)                                                                          agGCCACCTTCGAAGCGTTCTTGCCGCGGCGCGAAATCTGGGTAGGCACTCGCGGGAACCGCCCCGCCGACACCGTATTAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGa                                                          <  7:23095/135‑1 (MQ=255)                                                                          agGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGa                                                          <  1:288445/135‑1 (MQ=255)                                                                          agGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGa                                                          >  1:444850/1‑135 (MQ=255)                                                                            gCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgccccgc                                                                                                                              >  1:433953/1‑65 (MQ=255)                                                                            gCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAAccgccccgc                                                                                                                              <  2:433953/65‑1 (MQ=255)                                                                                    cGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTg                                                 >  7:43655/1‑134 (MQ=255)                                                                                              ttGCAGCGGCTCGAAATCGGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGc                                       >  3:88967/1‑134 (MQ=255)                                                                                              ttGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGCGCCTTGCTGAATACTTCGc                                       >  5:334786/1‑134 (MQ=255)                                                                                                   gCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGaaaa                                 <  2:342797/135‑1 (MQ=255)                                                                                                      gCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGAGTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGAAAACGc                              >  4:284579/1‑135 (MQ=255)                                                                                                         cgtaaTCTGTGTCGGCTCTGGCTGGAACCGCCCCGCCGACACCGAAATAGCCGCGAGCACCATACCCCTTCAACGCCAAGCAACGATTCTAAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGAAAACGCctt                           <  6:147147/132‑1 (MQ=255)                                                                                                         cGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCa                                                                                                    <  3:143595/62‑1 (MQ=255)                                                                                                         cGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCa                                                                                                    >  4:143595/1‑62 (MQ=255)                                                                                                         cGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGAAAACGCctt                           >  3:218144/1‑135 (MQ=255)                                                                                                         cGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGAAAACGCctt                           >  1:416959/1‑135 (MQ=255)                                                                                                                   gtAGGCACTCGCTGTAACCACCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAACCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGAAAACGCCTTCTTCAAACAg                 <  8:337128/135‑1 (MQ=255)                                                                                                                   gtAGGCACTCGCTGGAACCACCCCGCCTACACCGTAATAGCCGCGAGCACCTTACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGAAAACGCCTTCTTCAAACAg                 <  8:337126/135‑1 (MQ=255)                                                                                                                              cTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGAAAACGCCTTCTTCAAACAGCGGCAGGCCAt      >  1:459037/1‑135 (MQ=255)                                                                                                                               tGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGACCGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGACAACGCCTTCTTCAAACAGCGGCAGGCCAtt     >  1:173586/1‑135 (MQ=255)                                                                                                                               tGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGAAAACGCCTTCTTCAAACAGCGGCAGGCCAtt     >  5:212486/1‑135 (MQ=255)                                                                                                                               tGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGAACCATACACATTGAACGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGAAAACGCCTTCTTCAAACAGCGGCAGGCCAta     >  1:372019/1‑134 (MQ=255)                                                                                                                                  aaCCGCCCCGCCTACACCGTAATACCCGCTAGCACAATACCCCTTGAACGCCAAGCACCGATTCTTACACGGCGTTGGAGCCTTGCTGAATACTTCGCCGTATACGCCTTCTTCAAACAGCGGCAGGCCATTGgc  <  7:116639/135‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   TATGCCAGTACCGCGCTCGTTCAGGTAGCGGTTCATGCCATTGGCAAAGCCCGACATGGGCGTTTCACCCAGCAGGCCACCTTCGAAGCGTTCTTGCAGCGGCTCGAAATCAGTGTAGGCACTCGCTGGAACCGCCCCGCCGACACCGTAATAGCCGCGAGCACCATACCCCTTGAACGCCAAGCAACGATTCTTAAACGGCGATGGAGCCTTGCTGAATACTTCGCCGAAAACGCCTTCTTCAAACAGCGGCAGGCCATTGGC  >  ACB122/4373036‑4373299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑