| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,681,221 | A→C | 29.4% | Q162P (CAG→CCG) | yejF → | putative ABC transporter ATP‑binding proteinYejF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,681,221 | 0 | A | C | 29.4% | 28.5 / 2.0 | 31 | Q162P (CAG→CCG) | yejF | putative ABC transporter ATP‑binding proteinYejF |
| Reads supporting (aligned to +/- strand): ref base A (7/14); new base C (8/2); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATCCTCCTGCTGCACAAGGGCCTGACGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGG‑CCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCAGGCGCG > ACB122/4681089‑4681353 | aaaTCCTCCGGCGGCCCAAGGGCCTGCCGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGGCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGgccagc < 4:305171/135‑1 (MQ=255) aaTCCTCCTGCGGCACACGGGCCTGACGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGgccagcg < 8:222503/135‑1 (MQ=255) gcACAAGGGCCTGACGGGCAAGGAAGCGACTGCGCGCACCCTGGAGGTGCTGGATCTGGTCGGCATTCCCGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAAt < 8:133166/135‑1 (MQ=255) acacGGGCCTGACGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAAt < 3:196052/133‑1 (MQ=255) cgggCCTGACGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCg < 4:52812/134‑1 (MQ=255) aGGGCCTGACGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCg < 2:399476/135‑1 (MQ=255) ccTGACGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCCGCGCCACCGGGGAATTATCGccca > 8:95073/1‑133 (MQ=255) cGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCCCCCGCGGGGTAAGCTCGCCATGgcgc > 2:53544/1‑135 (MQ=255) cGGGCAAGGAAGCGACTGCGCGCACCCTGGAGGTGCTGGATATGGTCGGCGTTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGGCCCACGATTTGTCGGGCGGCCAGCGGCCGCGCGTCATGAAAGCCAGGgcgg > 4:86698/1‑134 (MQ=255) gCGACGGCGCGCACCCTGGTGTTGCTGGATAGGGTCGGCATGCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGG‑CCAACGAg < 6:356636/135‑1 (MQ=255) cTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCCGCGCCCGCGGGGAATGGTCGCCCTTGCGCGGG‑CCCACGCGCCCg > 6:200200/1‑135 (MQ=255) aCCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGGCCACCCCACCGGGGAATGATATCCATGGAGCTGG‑CCAACCAGCCCGAgcgtctg > 4:347994/1‑130 (MQ=255) tGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCCGCCCCAGCGGGGAATTATAGCCATGGCGCGGG‑GCACCCCGCCCCCGCGGCGGATTGGcgaccccc > 6:123912/1‑131 (MQ=255) gATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCCCGAATTGTCGGGCGGCCAGCGCCACCGGGTAATGATCGCCATTGCGCGGG‑CCAACGCGCCCGAGCAGCTGGTTTCCGCCGACCCg > 3:110998/1‑135 (MQ=255) gATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCCCCGCCCGCCGGTTATGCTCGCCCTGGCGCTGG‑CCAACCCGCCCGCGCGGGTGATTGCCGACGCGccc > 2:79025/1‑134 (MQ=255) gATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGGAATTCACGCCAAGGCCCAGG‑CCCACGAGCCCGCGCGGCTGATTGCCCACCAGccc > 8:51123/1‑134 (MQ=255) atatGGCCGGCATTCCCGAGCCGCGCAAGCGCCTCAAGGCCCGGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAAGGATCGCCATGGCGCTGG‑CCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGa < 3:107408/135‑1 (MQ=255) gggcGGCATTCCAGAGCCGCGCAAGCGCCGCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGAAAGGATCGCCATGGCGCGGG‑CCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCa < 4:195361/131‑1 (MQ=255) ggcGGCATCCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGGCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGG‑CCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACg < 1:375717/133‑1 (MQ=255) tCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCCGCGCCCGCGGGGAATGATCGCCATGGCGCTGG‑CCCACGCGCCCGCGCTGCTGCTTGCCGCCGAGCCGACCCCgg > 2:70731/1‑135 (MQ=255) cGGCATTCCAGAGCCGCGCAAGCGCCTCACGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGG‑CCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGgc < 4:94904/135‑1 (MQ=255) cAGAGCCGCGCAAGCGCCTCAAGGCCCGGCCCCACGATTTGTCGGCCGGCCAGCGCCAGCGGGAATTGATCGCCTGGGGGCGGG‑CAAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGAtg < 2:250624/135‑1 (MQ=255) cgCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGGCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGG‑CCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGa < 4:402985/134‑1 (MQ=255) aaGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCCGCGGGTAATGATCGCCATGGCGCGGG‑CCCACGCGCCCGCGCGGCTGATTGCCGACGCGCCGCCCCCGGCGCTGGATGTGACCGTGCAg > 2:38287/1‑135 (MQ=255) aGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCCGCGGGTTATGATCGCCATGGCGCTGG‑CCAACGCGCCCGAGCTGCGGATTGCCGCCGCGCCGCCCCCGGCGCGGGGTGTGACCGGGCAGCGGCAGATcc > 1:379939/1‑135 (MQ=255) cccACGAATTGTCGGGCGGCCCGCGCCAGCGGGGAATTATCGCCCTGGCGCTGG‑CCCACGCGCCCGCGCTGCGGCTTGCCGCCGAGCCGCCCCCCGCGCGGGATGTGGCCGTGCAGCTGCAGATCCTCGAgctgc > 1:149135/1‑135 (MQ=255) cccACGAATTGTCGGGCGGCCCCCCCCACCGGGGAATTATCGCCCATGCCCGGG‑CCAACCCGCCCCAGCTGCTGTTTGCCGACCCGCCGCCCCCCGCCCTGGATGTGACCCTGCCGCGGAAGATCCTCGAcctgc > 2:147614/1‑135 (MQ=255) ccACGTCTTGTCGGGCGCCCCGCGCAAGCGGGTAATGATCGCCATGGCGCTGG‑CAAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAgctgct < 1:376357/135‑1 (MQ=255) cACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATTATCGCCCTGGCGCTGG‑CCAACGAGCCCGAGCGGGGGATTGCCGCCGAGCCGCCCACGGCGCTGGATGTGACCGTGGAGCTGAAGCTCCTCGAgctgct > 6:244590/1‑134 (MQ=255) cGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGG‑CCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGc < 3:355317/135‑1 (MQ=255) cGGGCGCCCAGCGCCCGCGGTTATGGTGCGCCTGGGCGGGGG‑CCAACGCGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGc < 5:64675/135‑1 (MQ=255) gggCGGCCAGCGCCAGCGGGTAAGGAGCGCCATGGCGCTGG‑CCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCa < 7:95073/135‑1 (MQ=255) ggcggcCCGCGCCAGCGGGTAATGAACGCCAGGGCGCTGG‑CCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCAg < 6:251918/135‑1 (MQ=255) gccagcgccagcGGGAAATGATCGCCAGGGCGCGGG‑CCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCAGgcgc < 5:123912/135‑1 (MQ=255) ccagggccagggGGAATTGA‑CCCCATGGGGGCGGCCCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCAGGcgcg < 2:232028/135‑1 (MQ=25) ccagcgcccgcgGGTTATGATCGCCATGGCGCTGG‑CCAACGAGCCCGAGCTGCTGATTGGCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCGCGAGCTGCTCAAGGTATTGCAGGcgcg > 2:389646/1‑135 (MQ=255) ccagcgccagcgGGTTATGATCGCCATGGCGCTGG‑CCAACGCGCCCGCGCTGCTGATTGCCGCCGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCAGGcgcg > 7:165788/1‑135 (MQ=255) | AAATCCTCCTGCTGCACAAGGGCCTGACGGGCAAGGAAGCGACTGCGCGCACCCTGGAGTTGCTGGATATGGTCGGCATTCCAGAGCCGCGCAAGCGCCTCAAGGCCCTGCCCCACGAATTGTCGGGCGGCCAGCGCCAGCGGGTAATGATCGCCATGGCGCTGG‑CCAACGAGCCCGAGCTGCTGATTGCCGACGAGCCGACCACGGCGCTGGATGTGACCGTGCAGCTGAAGATCCTCGAGCTGCTCAAGGAATTGCAGGCGCG > ACB122/4681089‑4681353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |