| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,845,620 | T→G | 51.1% | intergenic (+47/+72) | apt → / ← recR | Adenine phosphoribosyltransferase/Recombination protein RecR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,845,620 | 0 | T | G | 51.1% | 2.0 / 2.3 | 24 | intergenic (+47/+72) | apt/recR | Adenine phosphoribosyltransferase/Recombination protein RecR |
| Reads supporting (aligned to +/- strand): ref base T (4/6); new base G (5/4); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGCTTTGCAGCCCATTCGC‑GGGTAAACCCGCTCCTACGAAAGCCGCGTA‑TGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATG > ACB122/4845493‑4845727 | gAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTTGGGC‑TGCTTTGCAGCCCATTAGCGGGGTAAACCCGGGCCTag > 7:463787/1‑133 (MQ=255) ccGGGGCGGGTCGGTTGCACGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGGGAGTATTTAGTGGGCTTTTGGGGC‑TGCTTTGCAGCCCATTCGC‑GGGTAAACCCGCTCCTACGAAAGCcgc < 7:124164/134‑1 (MQ=38) gTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGCTTTGCAGCCCATTCGC‑GGGTAAACCCGCTCCTACGAAAGCCGCGTA‑TGTTTTGTAg < 4:153168/135‑1 (MQ=255) ttGCACGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGCTTTGCAGCCCATTCGC‑GGGTAAACCCGCTCCTACGAAAGCCGCGTA‑TGTTTTGTAgg < 8:384273/135‑1 (MQ=255) gggTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGTGGC‑TGCTTTGCAGCCCATTCGCGGGGTAAACCC‑CGCCGACGCCAGGCGGGGATTGTTTTGT‑GGCGCGGGGTTTccc > 2:419214/1‑135 (MQ=255) gggTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGCTTTGCAGCCCATTCGC‑GGGTTAACCCGCGGCGACGCAAGCCGCCGT‑TGTTTTTTATGGGCGGGgtttgcc > 8:111411/1‑131 (MQ=25) cTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGCTTTGCAGCCCATTCGC‑GGGTAAAACCGCGCCGACGGAAGCCGCGGT‑TGTTTTGTAGGCCCGGGTTTTCCCGCGAATTGgc > 8:357957/1‑135 (MQ=255) tGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGCTTTGCAGCCCATTCGC‑GGGTTATACCCCCGCGCCCCAAGCCGCGTT‑TGGTTTGTATGCCCGGGGTTAACCGCGCATAGggcgcc > 8:433208/1‑135 (MQ=255) tGACCTAGTTTTCCCGCACCCTGTATGTAGTGGGCTTTTGGCGA‑CGCTTTCAAGCCAATTCGC‑GGGTAAACCCGCGCCCACCAACGCCGCGTA‑TGTTTGGAAGGGGCGGTTTAACCCGCTAATGGGCCGCCAAGc < 4:332312/135‑1 (MQ=255) tGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGCTTTGCATCCCATTCGC‑GGGTAAACCCGCTCCTACGAAACCCGCGGA‑TTTTTTTTATTCTCGGGTTTAAACGACAATTGGCCGGCatcc > 8:455124/1‑132 (MQ=25) gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGCTTTGCAGCCCATTCGC‑GGGTAAACCCGCTCCAACGCAAGCCGCGGA‑TTTTTTTTTGGCGCGGGGTTTCCCGCGCATGGGCCGCCACGCGGccc > 8:354528/1‑135 (MQ=37) gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGCTTTGCAGCCCATTCGC‑GGGTAAACCCCCTCCGACCACATCCGCCGA‑GGTTGTGGAGGCCCGGGTTTACCCCCGAATGGGCCGCCACGCGGccc > 7:159439/1‑135 (MQ=37) ccccTCCGCGTGTTTGGAGTGGGCTTTGGGGGC‑GGTTTGGCCGCCATTAGGC‑GGTTAAACCCCCCCCACCTAACGCCGGGTT‑TGTTTGGTCGGCGGGGGTATACCGGCTAATGGGCCGCCAAGCGGCCCCAATg < 6:124198/133‑1 (MQ=255) tGGGCTTTTGGGGC‑TGCTTTGCAGCCCATGCGC‑GGGTAACCCCGCTCCTCCGAAAGCCGCGTA‑TGTTTTGTCGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg < 7:39044/135‑1 (MQ=255) ggggCTTTGGGGGC‑TGCTTGCCAGCCCATTCGC‑GGGTAAACCCGCGCCAACGAACGCCGCTTT‑GGTTTTGTCGGCGCGGGTTAACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg < 2:113119/134‑1 (MQ=25) ggggCTTTGGGGGC‑GGCTTGGCAGCCCATTCGC‑GGGAAACCCCGCCCCTCCGAACGCCGCGTT‑TGTTTGGTCGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg < 4:233178/134‑1 (MQ=37) gggCTTTTGGGGC‑TGCTTTGCAGCCCATTCGC‑GGGTTAACCCGCGGCGGCGCAATCCGCGGA‑TTTTTTGTTGGCGCGGGTTTAGCCGCGGATGGGGCGCCACCGGGGCCCAAAGAATACGCGC‑GGAAAGGGCCgc > 4:55905/1‑134 (MQ=16) gggCTTTTGGGGC‑TGCTTTGAAGCCCATTCGC‑GGGTTAACCCGCTGCTACGCAAGCCGCGGA‑TGTTTTTTATGCGCGGGGTTAACCCCGGATGGGCCCCCCAGCCGGCCCAATGAATAAGAGCGGAATTGGGCCg > 2:123811/1‑135 (MQ=255) tGGGGCGTGCTTGCCCGCCCAGTCGC‑GGTAAACCCCGCCCCACCCAACGCCGCGTA‑GGTTTGGTCGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCt < 1:148384/135‑1 (MQ=25) ggggC‑GGCTTGGCCGCCCTTTCGC‑GGGAAAACCCGCGCCACCAACCGCCGGGTA‑TTTTTTGTCGGCGCGGTTAACCCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTg < 5:295709/134‑1 (MQ=255) ttGCAGCCCATTCGC‑GGGTAAACCCGCTCCTCCGAACGCCGCGTA‑TGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg < 2:51520/135‑1 (MQ=255) ttGCAGCCAATTCGG‑GGGAAACCCCGCACCTACCAACGCCGGTTA‑TGTTTTGTCGGCGGGGGTTAACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg < 6:409237/135‑1 (MQ=255) tgccAGCCAATTCGC‑GGGTAAACCGGCGCATACGAACGCCGCTTA‑TGTTTTGTCGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg < 7:111411/132‑1 (MQ=38) gccgCCCATTCGC‑GGGTAACCCCGCTCCTACGAACGCCGCGTA‑TGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg < 3:108142/130‑1 (MQ=255) | GAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGCTTTGCAGCCCATTCGC‑GGGTAAACCCGCTCCTACGAAAGCCGCGTA‑TGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATG > ACB122/4845493‑4845727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |