| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,874,709 | A→C | 34.2% | intergenic (+13/‑174) | CJ019_04568 → / → uacT_1 | hypothetical protein/Uric acid transporter UacT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,874,709 | 0 | A | C | 34.2% | 34.5 / 3.6 | 38 | intergenic (+13/‑174) | CJ019_04568/uacT_1 | hypothetical protein/Uric acid transporter UacT |
| Reads supporting (aligned to +/- strand): ref base A (10/14); new base C (9/5); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCC > ACB122/4874578‑4874843 | gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTAGCCAAAGGGGCCCAGGGGAACGGCCAGCCCCTCGCCCGGc > 5:341906/1‑135 (MQ=255)gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGGCAACTGCCAGCACCTCGCCCGGc > 3:115409/1‑135 (MQ=255)gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACAGGATCGCCAAAGGCGCCCACGTCAACTTCCAGCACCACCCCAGGc > 2:51108/1‑135 (MQ=255) aTTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAACGCGCCCAGGTCAACTGCCAGCACCACGCCAGGCaa > 5:454895/1‑135 (MQ=255) ttCCGGCGCATGCGGTTGCCTGCAATATCATGCCGGTGGGAAACATCACCCTGATGACCACCGAAGAGCGCATGCTGGTGGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc < 7:266458/135‑1 (MQ=255) ttCCGGCGAATGCGGTTGCCAGCCAGATCATGCCGCTGGGAAACATCACCCAGATGACCCCCTAAGAGCGCACGCTGGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc < 3:143347/135‑1 (MQ=255) aGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGGCAAACGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCg > 3:51298/1‑135 (MQ=255) aGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCGCGCCCCGCCACCCg > 3:384288/1‑135 (MQ=255) ccatggggTTGCCAGCCAGATCATCCCGCTGGGAAAAATCACCAAGATGACCACCGAAGTGCGCAAGCTGGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGcccc < 1:51108/129‑1 (MQ=255) gCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCCCCCAGATGACCACCGAAGCGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGGGCCCAGGTCAACAGACCCCCCCGCCCCCGGCAACCCGcccccccc > 3:148898/1‑133 (MQ=255) ccagcccgATCAGGCCGCGGGGCAAAATCACCCATATGACCCCCGAAGAGCGCACGCGGGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATc < 6:124864/135‑1 (MQ=255) cagccagATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCGCGCCAAACGCGCCCCGGTCAACTGCCAGCACCGCGCCCGGCAACCCGCCCCCCCACGGCATcc > 1:411010/1‑134 (MQ=255) gccagATCATGCCGCTGGGCAACATCACCCAGATGACCACCGACGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCAcc < 4:38741/135‑1 (MQ=255) gccagATCATGCCGCTGGGAAACATCCCCCAGATCACCACCGACGCGCGCACGCGCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCAcc < 4:384288/135‑1 (MQ=255) aTCATGCCGCGGGGAAACACCACCCAGATCACCACCGACGAGCCCAAGCGCGGCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTccc < 6:433033/135‑1 (MQ=255) ggaaaCATCACCCAGATCACCACCTAAGAGCGAATGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCgg < 6:142061/132‑1 (MQ=255) aGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCCAGGGCATCACCATCCCCTGCAGGAGCGGGCTTGCCCGCGaa > 3:165711/1‑135 (MQ=255) aGATGACCACCCAACGGCGCACGCGGGGCGGCGACTGGATCGCCAACGGCGCCCAGGTCAACTGACACCACCGCCCCCGCCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGGGGGCTTGCCCGCTaa < 4:103429/135‑1 (MQ=255) accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGGCAAAAGCGCCCAGGTCAAAAGACAGCCCCACGCCAGCCCACCCGCCCCCCAAGGGAATACCCTAACCCAGCACGAGCGGGCATGGCCGCCACGAGGc > 2:323990/1‑135 (MQ=255) accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGc > 2:389742/1‑135 (MQ=255) accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACAGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGTATGAACTTCCCCTGCAGGAGCGGGGTTGCGCGCGAAGAggg > 8:382016/1‑134 (MQ=255) tacgcgcgaAAGCTGTTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCCCCCCCCAAGGTAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAg < 8:308062/130‑1 (MQ=255) aaGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCgg > 5:143482/1‑102 (MQ=255) aaGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCCGCCACCCGCCCCCCACGGGAATCACCTTCCCCGGCAGGAGCGGGCTTGCCCGCGCAGAGGACGATTGCCCCCGccc > 6:77521/1‑134 (MQ=255) aaGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCgg < 6:143482/102‑1 (MQ=255) ggggCGAGGGGATCCACAACGGCGCCCTGGTCAACGGCCAGCACCTCGCCAGGCAACCCCCCCCCCACGGGAAGCACCTTCCCGGGCCGGAGCGGGCTTGCCGGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTc < 8:299294/133‑1 (MQ=255) ggCGACTGGATCGCCAAACGCCCCCAGGTCCACAGACCGCACCTCGCCCGGCCACCCGCCCCCCAACGGGCTCACCTTCCCCCGGACGACCCGGCTTTCCCGCCGAGCGGCCCCAAGCCCCCCCCACAAGCTCCa > 6:115586/1‑135 (MQ=255) agcGCAAACGGCGCCCAGTTCAACTGACCGCACCTCCCCCGGCAACCCCCCCCCCAAGGGAACCCCCTTCCCGTGCAGCCGGGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg < 5:448212/133‑1 (MQ=255) acccccAACGGCGCCCCGGTCAACTCCCCGCCCCCCCCCAGCCACCCCGCCCCCCACGGGAACCACCTCCCCCTGCCGGCGCGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg < 3:305507/131‑1 (MQ=255) ccAATGGCGCCCTGGTCAACTTACCGCACCTCGCCCGGCACCCCGCCCCCCATGGTAATCACCTTCCCCTGCAGGCGGGGGTTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGca < 2:333891/133‑1 (MQ=255) aGGTCAACTGACAGCACCTCGCCAGGCACCCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCCGCCTCAAGCTCCATGTGTTACGCAACCagcaagcaa > 4:419037/1‑132 (MQ=255) aGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCAt > 6:363534/1‑133 (MQ=255) tCAACTGACAGCACCTCGCCAGGCACCCCGCCCCCCAAGGGAATCACCTACCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 7:362109/135‑1 (MQ=255) tCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCACGGTAATCACCTCCCCCTGCAGCAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 6:447953/135‑1 (MQ=255) aaCTGACAGCACCCCGCCCGCCAACCCGCCCCCCAAGGCAATCACCTCCCCCTGCCGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgcgc < 1:389742/135‑1 (MQ=255) aCAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGCAGAGGCCGATAGCCCCAGCCTCAAGCGCCATGTGTTACGCAACAAGCACGCATCGAGCGCGCAg > 2:77879/1‑134 (MQ=255) aCCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCCGCCTCCAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCAt > 6:447464/1‑133 (MQ=255) tCGCCCGGCAACCCGCCCCCCACGGTAAACACCTGCCCCTGCAGGAGCGGGCTTCCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc < 6:454895/135‑1 (MQ=255) aGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGctgtct > 1:163244/1‑130 (MQ=255) | GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCC > ACB122/4874578‑4874843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |