| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,894,035 | T→G | 32.8% | intergenic (+31/‑467) | lrpC → / → pucI_2 | HTH‑type transcriptional regulator LrpC/putative allantoin permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,894,035 | 0 | T | G | 32.8% | 24.4 / 4.0 | 37 | intergenic (+31/‑467) | lrpC/pucI_2 | HTH‑type transcriptional regulator LrpC/putative allantoin permease |
| Reads supporting (aligned to +/- strand): ref base T (9/15); new base G (6/7); total (15/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGA > ACB122/4893907‑4894168 | tGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCcgc < 4:307248/135‑1 (MQ=255)tGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAACAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCcgc < 3:218945/135‑1 (MQ=255)gggACACCCTGCTCGACCTGTTTTTTGTCCTGCCCTACACCAAAAGGGCGATCTACACGACG‑CCCCGGTGCCAGGGGCGGTTGCCGCCAATGGATTGTTTGGCTCGTGTGCTGCCCTCTTCGCTGGGGGGCCcgc < 2:400718/134‑1 (MQ=255) gACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCACTTCGCAGGCGGGCCcgc > 3:99766/1‑133 (MQ=255) acCCTGCTCGACCTCATGAAGGTGCGGCCCGAGACAAATCCCCCGAGCATCAAGACCACCCCGGT‑CACGCGGCGGTTGCCGCCAATGGATTGATTGGCGCCTGTGCGGGCCTCTTCGCAGGCGGGCCCGCGAaga < 4:38358/135‑1 (MQ=255) cccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCCGGCGGGCCCGCGAagag < 4:99766/135‑1 (MQ=255) cccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCGCTTTGCCGGCGGGGCCCCGGagag > 1:19733/1‑135 (MQ=255) ggCTCGACCTCATGAAGGTGCGGCCCCCTCCAAAAACCGCGATCATCAAGACCCCCCCGGT‑AAAGCGGCGGTTGCCGCCAATGGATTGATTGGCGCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCTAAGAGGcc < 7:50767/134‑1 (MQ=255) tCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAg > 8:392753/1‑134 (MQ=255) tCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGt > 4:400907/1‑135 (MQ=255) tCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGt > 4:130075/1‑135 (MQ=255) gAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTACCACGCGTGGCCCCGCAGCCGCCACTACACGaaaaa > 4:32475/1‑134 (MQ=255) caaTGGGCTGGCCGAGACAAATACCGCGATCAGCAAGAAGACCCCGGT‑CAAGGGGCGTTTGCCGCCATTGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAg < 2:44204/134‑1 (MQ=38) aaTGTGCTGGCCGATACCAATACCGCCATCAACAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCAAATGTATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGaaa < 4:132779/132‑1 (MQ=39) aaTGTGCGGCCCGAGACCAAAACCGCCAGCAGCAAGACGCCCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCGCCTGGGCTGGCCTCTTCGCAGGCGGGCCCGCGAAGAGGCCAGTACAGGAAAAg < 1:265739/134‑1 (MQ=255) tGGCCGAGCCCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTAGTCCCCGGCCTGGCCGCGAACAGGCCCGTACCGGAAAAGCTGAGTAt > 3:132601/1‑135 (MQ=255) gCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCACGCGTGGCCGCGGAGAGGCCAGTACAGGAAAAGCTGAGTAttt > 4:182621/1‑135 (MQ=255) gCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCACATCGCCGGGGGGGCCGCGCAGAGGCCACTAAACGGAAAGCAGAGTtttt > 8:22764/1‑135 (MQ=255) gCCGACACCAAACCCGCCATCAACAACAACCCCCCGGT‑CAAGCGGGGGTTGCCGCAATTGTATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGGGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAttt < 4:301206/135‑1 (MQ=37) ccGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCCCGCGTGCCCGCGGAGCGGCCAGTACAGGAAAACCTGCGTAtttt > 6:360183/1‑135 (MQ=255) ccGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACaaaa < 7:392753/135‑1 (MQ=255) tcatcaAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCGCTTAGCCGGCGGGCCCCCCCAGAGGCCACTACAGGAAAAGCGGAGTATTTTTTTTTAACAAAAAAGTaa > 4:274931/1‑135 (MQ=255) tcatcaAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCACATAGCCCGCGGGGCCCGGCACAGGGCAGGACAAGCAAAGCCGGGGTTTTTTTTTTCACAAAAAccata > 3:174548/1‑131 (MQ=255) ggT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTAGCAGGCGTGCCCGCGGAGAGGGCCGGAACGGCAAAGCTGTGTATTTTTTTTTCAAAAAAACGTAACCATAGGTGGCCtttt > 4:96597/1‑135 (MQ=255) ccacgcggcgGTTGCCGCCAATGGATTGATGGGCGCCTGTGCGGGCCTCTCCGCCGGCGGGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTtg < 1:409642/131‑1 (MQ=255) aaGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCGTGGGAGGCGGGGCCGCGACGCGGGCCGGACAGGTAAAGCTGTGTTTTTTTTTTTAACAAAAACGTAACCAAATGTGGCATTTTtttg > 7:128047/1‑135 (MQ=255) gccAATGGATGGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAg < 3:282023/135‑1 (MQ=255) ggATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTg < 7:155118/104‑1 (MQ=255) ggATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTg > 8:155118/1‑104 (MQ=255) attgattgGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGcc < 3:400907/134‑1 (MQ=255) ttgattgGCTCCTGTGCTGGCCGCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGcccc < 7:365372/135‑1 (MQ=255) gcgggCCTCTTCGCCGGCGTGCCCGCGATGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACt < 4:199439/132‑1 (MQ=255) cgggCCTCTTCGCAGGCGGGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACtt < 4:16672/133‑1 (MQ=255) cTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACtt < 6:317187/135‑1 (MQ=255) cTGGCCTCTTCGCAGGCGGGCCCGCTAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACtt < 3:32475/135‑1 (MQ=255) ccTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCa < 3:281558/135‑1 (MQ=255) ctctTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCAc < 3:182621/135‑1 (MQ=255) tGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGa > 8:279068/1‑134 (MQ=255) | TGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGT‑CAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGA > ACB122/4893907‑4894168 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |