| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,097,064 | C→G | 40.6% | intergenic (+28/‑162) | CJ019_04792 → / → CJ019_04793 | Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,097,064 | 0 | C | G | 40.6% | 21.9 / 8.8 | 29 | intergenic (+28/‑162) | CJ019_04792/CJ019_04793 | Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (8/8); new base G (9/3); total (17/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGC‑ATATGTGCGCT‑GTT‑CGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGC > ACB122/5096931‑5097189 | cACCGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGccccg > 7:398585/1‑134 (MQ=255) gTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCTCAACGACTTCATCATGTGGGCACGCACTGACAAGGTGGCGTAAACGGCTGAAGGCCGGAAGTAAACACCAGACGGAAGGGGCCAGAGGCCCCttttt < 4:178841/135‑1 (MQ=255) gTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGGGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCttttt < 5:55385/135‑1 (MQ=255) tGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGgtttt > 5:57046/1‑134 (MQ=255) gACCTGGTGGATCCACTCTATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCTGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGTGCCAGAGGCCCCTTTTTTc < 6:149100/135‑1 (MQ=255) gACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCttttt > 8:36630/1‑133 (MQ=255) gACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTc < 3:179457/135‑1 (MQ=255) gACCTGGTGGAGCCACTCGAGCAACGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACGGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTc < 1:155878/135‑1 (MQ=255) cTGGTGGAGCCACTCGATCAAACGCCGGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGACGGACGGGGCCAGAGGCCCCTTTTTTCATg < 6:378545/135‑1 (MQ=255) tggtggAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTGTTTTCATgg > 2:54791/1‑135 (MQ=255) cACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGC‑ATAtgt < 4:462758/135‑1 (MQ=255) cTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGTTTTTCATGGGGC‑ATATGTgc > 3:152384/1‑135 (MQ=255) gCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGTTTTTCATGGGGC‑CTATGTGGGCG‑GTT‑CGccc > 3:352584/1‑135 (MQ=255) aCCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTCATGGGGG‑ATATGTGGGCT‑GGT‑CGCCCCCaaaa > 8:298287/1‑135 (MQ=255) cGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTCATGGGGC‑ATATGTGCGCT‑GTT‑CGCCCGCAAAACCGACaa > 8:34550/1‑135 (MQ=255) cGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTAATGGGGG‑ATTTGTTCGCT‑GGT‑GGCCCGCCAAACCGCCac > 6:42251/1‑134 (MQ=255) cTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTTCTGGGGC‑ATATTTTCGGT‑GTT‑CGCCCGCAAAACCGCCACACg > 8:189506/1‑135 (MQ=255) tcatGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGGTTTTTATTGGGG‑GTCTGTGAGCG‑GTT‑GGCGCGCCAAACCGCCCAaccacccc > 5:293019/1‑133 (MQ=255) gcacgcacTGCCAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTAATGGGGC‑ATATGTGCGCT‑GTT‑CGCCCGCAAAACCCACAAACGACCGGGaaaaaaa > 7:53871/1‑135 (MQ=255) gcacgcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGTTTTTCATGGGGC‑ATATGTGCGCT‑GTT‑GGCCCGCAAAACCGACAAACGACCGGGaaaaaaa > 7:61408/1‑135 (MQ=255) gcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTTATGGGGC‑CTATTTTCGCTGGTG‑GGCCCCCAAAACC‑CCAAACCACCGGGAAAAAAAcaa > 2:348787/1‑132 (MQ=255) acTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTCATGGGGG‑ATATTTGGGCT‑GTT‑CGCCCGCAAAACCGACAAACGACCGCGAAAAAAACCcaaa > 1:54194/1‑134 (MQ=255) ggTGGCGGAAAGGTCTGAGGGCCGTAAGTAAACACAACATGGACGGCGCCAGCGCCCCATTTTTACATGGGTC‑ATTTGGGCG‑T‑GTTCCCCCCGCAAAACCGCCAAACGACCGGGAAAAAAACCAAACTGTCATcc < 8:220559/135‑1 (MQ=255) aaGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGTGTTTGATGGGGC‑ATATGTGCGCT‑GTT‑GGCCCGCCAAACCGACCAACGCCCGGGAAAAAAACCAAACTGTCATCCAGCCTTGTGTATcaggc > 4:7494/1‑131 (MQ=255) ggCCGTAAGAAAACACCACAAGGATGGGGCCAGCGGCCCCTTTTTACTTGGGGC‑ATATGGGCGCG‑GTG‑CGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGccc < 3:120126/135‑1 (MQ=255) aaGTAAACACCAGAAGGAAGGGCCCAGAGGCCCGTTTTTTCATGGGGC‑ATATGTGCGCT‑GTT‑CGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCtt < 7:309248/135‑1 (MQ=255) aaggaaggGGCCAGAGGCCCCGTTTTTCATGGGGG‑ATATTTGCGCT‑GGT‑GGCCCGCAAAACCGCCAAACCACCCGGAAAAAAAACAAACAGTCAACCAGACATGTGTATACTGCCCCCCCGTTTTGAATCAACa > 4:281020/1‑134 (MQ=255) acggggCCAGCGGCCCGTTTTTTCATGGGGC‑ATAGGTGCGCT‑GAG‑CGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAAcgc < 8:128799/133‑1 (MQ=255) ggCCAGAGGCCCGGTTTTTGATGGGGC‑ATATGTGCGCT‑GTT‑CGCCCGCAAAACCGACACACGCCCGGGAAAAAAACCAAACTGGGATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGATGCTACCAACGCGccg > 8:223283/1‑134 (MQ=255) acgagGCCCGTTTTTAC‑TGGGGCAATAGGGGCGGG‑GTC‑CGCCCGAAAACCCCCCAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGgc < 2:18955/133‑1 (MQ=255) | CACCGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGC‑ATATGTGCGCT‑GTT‑CGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGC > ACB122/5096931‑5097189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |