Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,252,157 T→G 25.8% D323A (GAC→GCC)  fabV ← Enoyl‑[acyl‑carrier‑protein] reductase [NADH]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,252,1570TG25.8% 63.9 / 5.0 52D323A (GAC→GCC) fabVEnoyl‑[acyl‑carrier‑protein] reductase [NADH]
Reads supporting (aligned to +/- strand):  ref base T (2/35);  new base G (3/12);  total (5/47)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCAGTCAGCTCGAACAGGTTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATCGACAGGTACAGTGGCATCACCGGGATGGCCGAGCTGGC  >  ACB122/5252023‑5252287
                                                                                                                                      |                                                                                                                                  
agtcagtcAGCTCGAACAGGTTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGt                                                                                                                                    <  1:222766/135‑1 (MQ=255)
  tcagtcagCTCGAACAGGTTCTCGGTGGTCACCTGTTGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCg                                                                                                                                  <  7:35617/135‑1 (MQ=255)
  tcagtcagCTCGAACAGGTTCTCGGTGGTCACCTGTGGCCACAGTGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCg                                                                                                                                  <  3:339970/135‑1 (MQ=255)
  tcagtcagCTCGAACAGGTTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGGCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCg                                                                                                                                  <  5:436051/135‑1 (MQ=255)
  tcagtcagCTCGAACAGGGTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTc                                                                                                                                   <  6:407656/134‑1 (MQ=255)
             gAACAGGTTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCCCCCGCGGTGCgggtca                                                                                                                        >  1:290148/1‑130 (MQ=255)
             gAACAGGTTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCCGCGGGGCGGTTCAt                                                                                                                       >  7:303109/1‑135 (MQ=255)
              aaCAGGTTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACa                                                                                                                        <  3:203791/133‑1 (MQ=255)
              aaCAGGATCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCTCGCAGTTCCCAGTCCTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGGCGGTGCGGTACa                                                                                                                        <  1:63286/133‑1 (MQ=255)
               aCAGGTTCTCGGTGGTCACCTGTGGCCACAGGGCATTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCATTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCAACGGCGGTGCGGTACATAc                                                                                                                     <  4:360956/135‑1 (MQ=255)
                 aGGTTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACgg                                                                                                                   <  4:308667/135‑1 (MQ=255)
                 aGGTTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACgg                                                                                                                   <  4:314618/135‑1 (MQ=255)
                   gtgctcGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTc                                                                                                                 <  6:323837/132‑1 (MQ=255)
                   gTTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTc                                                                                                                 <  6:282512/135‑1 (MQ=255)
                   gTTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGGCGGTGCGGTACATACGGTc                                                                                                                 <  2:171752/135‑1 (MQ=255)
                          gtggtCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGt                                                                                                            <  2:163247/133‑1 (MQ=255)
                              tCACCTGTGGCCACAGGGCCTTGCAGGCGTGTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGc                                                                                                      <  5:313368/135‑1 (MQ=255)
                                  cTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTc                                                                                                  <  1:173940/135‑1 (MQ=255)
                                  cTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGGCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTc                                                                                                  <  3:309402/135‑1 (MQ=255)
                                   tgtgGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCg                                                                                                 <  5:33276/135‑1 (MQ=255)
                                   tgtgGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGGCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCg                                                                                                 <  2:435809/135‑1 (MQ=255)
                                                 cTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGt                                                                                   <  3:299508/135‑1 (MQ=255)
                                                  ttGCAGGCGTTTTGCACGGCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTg                                                                                  <  5:441027/135‑1 (MQ=255)
                                                         cggtttGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGt                                                                           <  6:55707/132‑1 (MQ=255)
                                                             ttGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGGACATACGGTCGCGGGACATGCGGGGGCGCGGGGCCTGGGGGGCGTCGTGGac                                                                       >  2:54849/1‑135 (MQ=255)
                                                               gCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAaca                                                                      <  8:461057/134‑1 (MQ=255)
                                                                cACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGCCCTCTGCCGGCGCCCCGGCCGTGCGGGACATACGGTCGCGGGACAGTGGGGCGCGGGGGTCCGGGGGGCCGTCGTGGACAcc                                                                    >  8:279719/1‑135 (MQ=255)
                                                                        cACGCAGTTCCTAGGCGTCCAGGCGCAGGCGCCCCTTCTCGGCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCt                                                            <  7:286223/135‑1 (MQ=255)
                                                                        cACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTTGTCGACCTCTGCCGGCTCACCGGCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCt                                                            <  2:290086/135‑1 (MQ=255)
                                                                        cACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTTGTCGACCTCTGCCGGCGCACCGGCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCt                                                            <  2:283254/135‑1 (MQ=255)
                                                                        cACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCt                                                            <  8:437271/135‑1 (MQ=255)
                                                                        cACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGGCGACCTCTGGCGGCGCACCGGCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCt                                                            <  4:168721/135‑1 (MQ=255)
                                                                          cGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGc                                                          <  3:403200/135‑1 (MQ=255)
                                                                                       cgtcCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGa                                             <  3:404000/135‑1 (MQ=255)
                                                                                       cgcccAGGCGCCGGCGCCCCGTCTCGTCGACCTCTGCCGGCGCACCTTCGGGTGGGTACATACGGGCGAGGTACATGCGGTCGAGCTGGTCCTGGGGGCCTTCGTTAACACCTTTCTCCTGAATGATGTTGAAGa                                             <  2:450172/132‑1 (MQ=255)
                                                                                         tcCAGGCGCCGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGGCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGAcc                                           <  6:29473/135‑1 (MQ=255)
                                                                                            cggcgcaggcgcCCGTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATc                                        <  6:446672/134‑1 (MQ=255)
                                                                                            aggcgcaggcgcCCTTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGTTACTTACGGTCGCGGTACATGGGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATc                                        <  4:282909/135‑1 (MQ=255)
                                                                                            aggcgcaggcgcCCCTTCTCGTCGACCTCTGCCGGCGCACCGTGGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATc                                        <  4:120273/135‑1 (MQ=255)
                                                                                            aggcgcaggcgcCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATc                                        <  6:440213/135‑1 (MQ=255)
                                                                                            aggcgcaggcgcCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATc                                        <  7:295276/135‑1 (MQ=255)
                                                                                            aggcgcaggcgcCCCTTCGCGGCGCCCTCTGCCGGCGCACCGGCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATc                                        <  8:193791/135‑1 (MQ=255)
                                                                                              gcgcaggcgcCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATCGa                                      <  4:207353/135‑1 (MQ=255)
                                                                                               cgcaggcgcCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATCGAc                                     <  1:319482/135‑1 (MQ=255)
                                                                                                   ggcgcCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATCGACAGGt                                 <  1:54849/135‑1 (MQ=255)
                                                                                                              tcgtcgACCTCTGCCGGCGCACCGGCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATCGACAGGTACAGTGGCAt                       <  5:112505/134‑1 (MQ=255)
                                                                                                                gtcgACCTCTGCCGGCGCACCGGCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATCGACAGGTACAGTGGCATCAc                    <  5:235991/135‑1 (MQ=255)
                                                                                                                     ccTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATCGACAGGTACAGTGGCATCACCGGGa               <  2:50332/135‑1 (MQ=255)
                                                                                                                     ccTCTGCCGGCGCACCGGCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATCGACAGGTACAGTGGCATCACCGGGa               <  2:306271/135‑1 (MQ=255)
                                                                                                                     ccTCTGCCGGCGCACCGGCGGGGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATCGACAGGTACAGTGGCATCACCGTGa               <  4:97666/135‑1 (MQ=255)
                                                                                                                            cGGCGCACCGTCGGTGCGGTACATCCGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGGGCCTTCGTTAACACCTTTCTCCTGCATTATCTTGAAGACCATCGACAGGTACAGTGGCATCACCGGGATGGcc          <  1:229110/133‑1 (MQ=255)
                                                                                                                               cgcACCGTCGGTGCGGTACATAGGGGCGCGGTACTTGCGGGCGAGCTGGTCCGGGGTGCCTTCGTGAACACCTTTCTCCTGCATCAGCTTGAAGACCATCGACAGGTACAGTGGCATCACCGGGATGGCCGAGCt     <  1:219358/135‑1 (MQ=255)
                                                                                                                                  aCCGTCGGTGCGGTTCATACGGTCGCGGTACAATCGGTCGAGCAGGTCCTGGGTGGCTTCGTGAACACCATTGTGCTGCAAGAAATTGAAGAACCTCGCAACGTTAAGTGGCATAACCCGGCTTGCCGAGCTGGc  >  4:340369/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                  
AGTCAGTCAGCTCGAACAGGTTCTCGGTGGTCACCTGTGGCCACAGGGCCTTGCAGGCGTTTTGCACGTCGTCACGCAGTTCCCAGTCGTCCAGGCGCAGGCGCCCCTTCTCGTCGACCTCTGCCGGCGCACCGTCGGTGCGGTACATACGGTCGCGGTACATGCGGTCGAGCTGGTCCTGGGTGCCTTCGTGAACACCTTTCTCCTGCATGATCTTGAAGACCATCGACAGGTACAGTGGCATCACCGGGATGGCCGAGCTGGC  >  ACB122/5252023‑5252287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑