Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	5,478,784	G→A	5.4%	M299I (ATG→ATA)	purD →	Phosphoribosylamine‑‑glycine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,478,784	0	G	A	5.4%	236.7 / 2.6	74	M299I (ATG→ATA)	purD	Phosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base G (39/29);  new base A (2/2);  total (43/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGCATGGCCGAGGAAGGCAACGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGAGCCTGGGCGTGGTCCTGGCAGCGGGCGGTTACCCGGGCGA  >  ACB122/5478654‑5478915                                                                                                                                   |                                                                                                                                    gcgGCATGGCCGAGGAGGGCAACGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCAtgctgc                                                                                                                                 >  4:161363/1‑135 (MQ=255) gcgGCATGGCCGAGGAAGGCAACGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCAtgctgc                                                                                                                                 <  5:7096/135‑1 (MQ=255)   ggCATGGCCGAGGAAGTCAACGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTgcgc                                                                                                                               <  6:379067/135‑1 (MQ=255)   ggCATGGCCGAGGAAGGCAACGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCAtgctgc                                                                                                                                 <  6:307339/133‑1 (MQ=255)   ggCATGGCCGAGGAAGGCAACGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCAtgctgc                                                                                                                                 >  5:307339/1‑133 (MQ=255)            gAGGAAGGCAACGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGCCCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGt                                                                                                                        >  2:421921/1‑133 (MQ=255)            gAGGAAGGCAACGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGt                                                                                                                        >  6:15959/1‑133 (MQ=255)            gAGGAACGCAACGTCTACCCCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCg                                                                                                                      <  2:448697/135‑1 (MQ=255)             aGGAAGGCAACGTCTACACCGGCTTCCGCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGa                                                                                                                     <  2:252933/135‑1 (MQ=255)                aaGGCAACGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGcc                                                                                                                  >  3:342226/1‑135 (MQ=255)                     aaCGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTc                                                                                                             <  5:136000/135‑1 (MQ=255)                     aaCGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACACGAAGGGCATCGATTTCAACTGGCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTc                                                                                                             <  3:431712/135‑1 (MQ=255)                       cGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCct                                                                                                           >  4:156920/1‑135 (MQ=255)                                    ttCCTCTACGCCGGGCTGTAGATCGACAAGGCGGGCAACCCCAAGGTCATCGATTTCAACTGCCGCGTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGcc                                                                                              <  7:216124/135‑1 (MQ=255)                                    ttCCTCTACGCCGGCCTGATGATCCACCAGGCGGGCAACCCCAAGGTCTTCGAGTTCAACTGCCGCGTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGcc                                                                                              <  5:321353/135‑1 (MQ=255)                                    taccTCTACGCCGGCCTGATGATCGACACGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGcc                                                                                              <  4:315477/133‑1 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCACCTGCCGCTTCGGCGACCCGGAAACCACGCCGGTCAAGCTGCGCCTGGAGTCGAGGCTGGTACGGCTTGTGGAAGCCg                                                                                             >  2:455299/1‑135 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGCCCCGGAAACCCCGCCGGTCATGCTGCGCCTGGAGTCGAGCCGGCTCCTGCGGGAGGAAGCCg                                                                                             >  3:360089/1‑135 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCCGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGCAGCCg                                                                                             >  5:115589/1‑135 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGACGcc                                                                                              >  6:123127/1‑134 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGcc                                                                                              >  4:74753/1‑134 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGcc                                                                                              >  7:233496/1‑134 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGcc                                                                                              >  6:33586/1‑134 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGcc                                                                                              >  8:412076/1‑134 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGcc                                                                                              >  8:63158/1‑134 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCg                                                                                             >  3:128265/1‑135 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCg                                                                                             >  7:208204/1‑135 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCg                                                                                             >  3:67088/1‑135 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTACGGGTGGTGGAGGCCg                                                                                             >  5:395910/1‑135 (MQ=255)                                     tCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCGGGAGTCGCGCCGGGTCCGGCGGGTGGCGGCCg                                                                                             >  5:264112/1‑135 (MQ=255)                                     tCCTCTACGCCGGCCTGAAGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTACGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGACTCGAGCCGGGTCCTGCTGGTGGAAGcc                                                                                              >  1:377360/1‑134 (MQ=255)                                      ccTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGc                                                                                            >  7:40584/1‑135 (MQ=255)                                       ctctACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGc                                                                                            <  3:40946/134‑1 (MQ=255)                                             gCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCg                                                                                       <  7:412076/133‑1 (MQ=255)                                                ggCCTGATGATCGACAAGGCTGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAg                                                                                  <  6:47098/135‑1 (MQ=255)                                                ggCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAg                                                                                  <  4:151525/135‑1 (MQ=255)                                                    tgatgaTCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAACCCCAGCCGGTCATGCTGAGCCTGGAGTCGATCCTGGACCTGCTGGTGGAAGCCGCATTCGCCAACGCAc                                                                              >  7:7929/1‑135 (MQ=255)                                                    tgatgaTCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATTCTCCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGc                                                                                >  3:190252/1‑133 (MQ=255)                                                    tgatgaTCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGTTCCTGCTGGTGGAAGCCGCATTCGCCAAGTCAc                                                                              >  7:358275/1‑135 (MQ=255)                                                    tgatgaTCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCAc                                                                              >  3:139034/1‑135 (MQ=255)                                                    tgatgaTCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCCGGTGGAAGCCGCATTCGCCAAGGCAc                                                                              >  3:284856/1‑135 (MQ=255)                                                    tgatgaTCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCAGGTCCTGCTGGTGGCACCCGCAATCGCCACGGccc                                                                              >  6:303229/1‑133 (MQ=255)                                                       tgagCGACAAGCCGGGCAACCCCAAGGTCATCGAGGTGAACTGCCGCTGCGGCGACCCGGAAACCCAGCCGGTCAAGCGGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACt                                                                             <  4:427322/133‑1 (MQ=255)                                                        gatCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGa                                                                          <  5:303229/135‑1 (MQ=255)                                                           cGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACaa                                                                       <  8:127451/135‑1 (MQ=255)                                                                 ggCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGa                                                                 <  1:79571/135‑1 (MQ=255)                                                                   cGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAg                                                               <  3:182781/135‑1 (MQ=255)                                                                        aaCCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCCGCCGGGCATACTGCGCCTGGAGTCGAGCCGGGTCCTGCGGGGTGAAGCCGCCTTCGCCAAGGCACTGGCCAAGGTCGACGCACAg                                                          >  5:400822/1‑135 (MQ=255)                                                                        aaCCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATACTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCTCAGTCGCCACGGCACGGGACAAGGTCGAAGCACAg                                                          >  5:407762/1‑135 (MQ=255)                                                                         aCCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGt                                                         >  4:281050/1‑135 (MQ=255)                                                                            ccAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGATTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTggg                                                      <  5:15959/135‑1 (MQ=255)                                                                             cAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGa                                                     <  7:386554/135‑1 (MQ=255)                                                                                  tCATCGAGTTCACCTGCCGCTTCGGCGACCCGGAACCCCAGCCGGTCATGCTGCGCCGGGAGTCGAGCCTGGTCCTGCGGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCcgc                                                <  5:64414/135‑1 (MQ=255)                                                                                       gAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATTCTGCGCCTTGAGTCGAGCCAGGTACAGGTTCTTGTAGCCGCATTAGCCAAAGCACATGACAACGGCGAAGCACACAGGGACCcccgcccg                                           >  5:373734/1‑135 (MQ=255)                                                                                       gAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGcc                                             >  7:357999/1‑133 (MQ=255)                                                                                       gAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGcc                                             >  7:211605/1‑133 (MQ=255)                                                                                       gAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCg                                           >  5:318092/1‑135 (MQ=255)                                                                                       gAGTTCAACTACCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCg                                           >  6:234371/1‑135 (MQ=255)                                                                                        aGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGGCTGGGCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCCCAGTGGGACCCGCGCCCgc                                          >  3:287040/1‑134 (MQ=255)                                                                                        aGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGCAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCccccca                                          >  2:34671/1‑133 (MQ=255)                                                                                        aGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGcc                                                                                              >  4:152610/1‑83 (MQ=255)                                                                                        aGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGcc                                                                                              <  3:152610/83‑1 (MQ=255)                                                                                        aGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCCAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGa                                          >  3:230611/1‑135 (MQ=255)                                                                                        aGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGa                                          >  6:154622/1‑135 (MQ=255)                                                                                        aGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCCGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGa                                          <  4:284856/135‑1 (MQ=255)                                                                                        aGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCCGGTGCTGCTGGGGGTTGCCGCCTTCGCCCAGGGCCAGGCCAACGTCGAACCCCAGTGGGcccccccccccc                                          >  5:283365/1‑133 (MQ=255)                                                                                             aaCTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGGCCTGGTGGTGGAAGCCGCCTTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGAGCCTg                                     >  3:130197/1‑135 (MQ=255)                                                                                              ccttccGCTTCGGCGCACCGGAAACCCCGCCGGTCATACTGCGCCTTGAGTCGAGCCTGGTCCTGCTGGTGGACGCCGCATTCGCCACGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGAGCCTgg                                    <  6:400822/131‑1 (MQ=255)                                                                                              aCTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGAGCCTgg                                    <  5:429018/135‑1 (MQ=255)                                                                                              aCTGCCGCTTCGGCGACACGGAAACCCAGCCGGTCCTACTGCGCCTGGCGTCGAGCCGGGGCCTGCTGGTGGCCGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGAGCCTgg                                    <  6:407762/135‑1 (MQ=255)                                                                                               cTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGa                                                                          <  6:347115/96‑1 (MQ=255)                                                                                               cTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGa                                                                          >  5:347115/1‑96 (MQ=255)                                                                                                           cGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGAGCCTGGGCGTGGTCCTGGc                       <  8:140481/135‑1 (MQ=255)                                                                                                                          gCCGGGCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCGAAGGCACTGGACAAGGTCGAAGCAGAGGGGGACCCGCGCCCGAGCCTGGGCGTGGTCCTGGCAGCGGGCGGTTAccc        <  3:26960/135‑1 (MQ=255)                                                                                                                              tgcATGCGGCGCCTGGAGTCGAGCCTGGGCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGAGCCTGGGCGTGGTCCTGGCAGCGGGCGGTTACCCGGGc    <  2:377360/133‑1 (MQ=255)                                                                                                                              ggcATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGGGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGAGCCTGGGCGTGGTCCTGGCAGCGGGCGGTTACCCGGGc    <  2:209577/133‑1 (MQ=255)                                                                                                                               tCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGAGCCTGGGCGTGGTCCTGGCAGCGGGCGGTTACCCGGGCg   <  4:128265/135‑1 (MQ=255)                                                                                                                                 aTGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGAGCCTGGGCGTGGTCCTGGCAGCGGGCGGTTACCCGGGCGa  >  2:267986/1‑134 (MQ=255)                                                                                                                                   |                                                                                                                                    GCGGCATGGCCGAGGAAGGCAACGTCTACACCGGCTTCCTCTACGCCGGCCTGATGATCGACAAGGCGGGCAACCCCAAGGTCATCGAGTTCAACTGCCGCTTCGGCGACCCGGAAACCCAGCCGGTCATGCTGCGCCTGGAGTCGAGCCTGGTCCTGCTGGTGGAAGCCGCATTCGCCAAGGCACTGGACAAGGTCGAAGCACAGTGGGACCCGCGCCCGAGCCTGGGCGTGGTCCTGGCAGCGGGCGGTTACCCGGGCGA  >  ACB122/5478654‑5478915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑