Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	5,562,303	A→C	17.8%	L220R (CTG→CGG)	nnr ←	Bifunctional NAD(P)H‑hydrate repair enzyme Nnr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,562,303	0	A	C	17.8%	172.9 / 5.6	80	L220R (CTG→CGG)	nnr	Bifunctional NAD(P)H‑hydrate repair enzyme Nnr
Reads supporting (aligned to +/- strand):  ref base A (21/42);  new base C (14/2);  total (35/45)
Fisher's exact test for biased strand distribution p-value = 1.28e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGCCCCAGGCATCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCGGGTG  >  ACB122/5562170‑5562430                                                                                                                                      |                                                                                                                                ggccccccAGGCACCCAGCCCTGGGCCCAACCGGGCGCCCACCACGCCAGCCAGCACACCGCCCCGGCCGGCGCGGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAg                                                                                                                                <  1:94706/131‑1 (MQ=255)  gcgcCCCAGGCATCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCCTCGCGGGGTGCCCGCGCGCGCCCCACGCCCGCTGCCCGCTCGGGGCGGCCCCCAgg                                                                                                                               >  2:205639/1‑135 (MQ=255)  gcgcCCCAGGCATCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACCACGCCAGCTGCCCGCTCGGGGCTGCCACCAgg                                                                                                                               >  2:386481/1‑135 (MQ=255)  gcgcCCCAGGCATCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCCCCCgg                                                                                                                               >  6:423483/1‑135 (MQ=255)  gcgcCCCAGGCATCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCGCGCACCACGCCAGCTGCCCGCACGGGGCTGCCCCCCgg                                                                                                                               >  5:340787/1‑135 (MQ=255)  gcgcCCCAGGCATCACGGCCGTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATAGCGGGGTGCCCGCGCTCGCCAACCGCCCGCTGCCCGCGCGGGGCTGCCCCCAgg                                                                                                                               >  6:256003/1‑135 (MQ=255)    gcCCCAGGCATCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCCTCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGt                                                                                                                             >  8:154115/1‑135 (MQ=255)      ccccGGCACCCCGGCCTGGGGCCACCAGGCCCGCCCGCACCCCCGCCCCCACCGCCCCCCGGCCGGCCCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGc                                                                                                                           <  3:175126/135‑1 (MQ=255)      cccAGGCATCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCCCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGc                                                                                                                           <  8:292025/135‑1 (MQ=255)      cccAGGCACCCCGCCCTGGGGCCAACAGGGCTGCCAGCACGCCTGTCAGCACAGCCCCCCGCCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGc                                                                                                                           <  6:291928/135‑1 (MQ=255)           gCATCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATAGCTGGGTGCCCGCGCGCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCACGGTGCCGGcc                                                                                                                      >  5:132070/1‑134 (MQ=255)            cATCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCCTCGCTGGGTGCCCGCGCTCGCCCACCGCCCGCTGCCCGCTCGGGGCTGCCCCCCGGGTGCCGGccc                                                                                                                     >  3:242255/1‑133 (MQ=255)            cATCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCCCAACGCCAGCTGCCCGCGCGGGGCTGCCACCCGGGTGCCGGCAc                                                                                                                     >  1:31979/1‑135 (MQ=255)              tCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCCCAACGCCCGCTGCCCGCTCGGGGCTGCCACCCGGGTGCCGGccccg                                                                                                                   >  5:126121/1‑134 (MQ=255)               ccAGGCGTTGGGCCAACAGGGCTGCCAGCACGCCAGCCAGCACATCGCCCAGGCGGGCGCGGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCtt                                                                                                                  <  7:58523/135‑1 (MQ=255)               ccAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGGCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCtt                                                                                                                  <  8:97863/135‑1 (MQ=255)                cAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCCGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCttt                                                                                                                 <  2:359945/135‑1 (MQ=255)                 aGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCCTCGCGGGGGGGCCGCGCGCGCCCCCCGCCCGCTGCCCGCTCGGGGCGGCCCCCCGGGGGCCGGCCCCtggg                                                                                                                >  3:270154/1‑132 (MQ=255)                      ttGGGCCAAAAGGGCTGCCAGCACGCCAGTCAGCACAGCGCCCAGGCCGGCTCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTac                                                                                                           <  7:344921/135‑1 (MQ=255)                         ggCCAACTGGGCTCCAACCACGCCAGTCAGCACTCCGCCCTGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTAcaca                                                                                                        <  1:271531/135‑1 (MQ=255)                         ggCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTacac                                                                                                         <  8:437285/134‑1 (MQ=255)                         gcccAACAGGGGTGCAAGCCCGCCAGTCAGCAACTCGCCCGGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCGCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTacac                                                                                                         <  5:115262/132‑1 (MQ=255)                           ccAACCGGGCGGCCAGCACGCCAGGAACCACATCGCCCCGGCGGGCGCGGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGa                                                                                                      <  8:33810/135‑1 (MQ=255)                           ccAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCGGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCTCAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGa                                                                                                      <  3:325664/135‑1 (MQ=255)                           ccAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACACCGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACCCAGa                                                                                                      >  3:121280/1‑135 (MQ=255)                            cAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCCCCAGGGTGCCGGCACCTGTGAGTACACAGAc                                                                                                     >  5:145353/1‑135 (MQ=255)                                   gCTGCCAGCAGGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTta                                                                                              <  2:316063/135‑1 (MQ=255)                                     tGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCCTCGCTGGGTGCCCGCGCTCGCCCCACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCCCCTTTGAGTACACAGACGCTGTtata                                                                                            >  4:78428/1‑135 (MQ=255)                                       ccAGCACGCCAGTCAGACCATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGACCGCGCTCGCACAACGCCACCAGCCCGCTCGGGGCTGACACCAGGGTGCCGGCACCATTGACTACACAGACGCTGTTATAAc                                                                                          >  8:457679/1‑135 (MQ=255)                                          gCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCCGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGgc                                                                                       >  4:190618/1‑135 (MQ=255)                                                      gcactgcGCCCCGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTgc                                                                           <  2:280419/129‑1 (MQ=255)                                                      gCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCCGGGTGCCGGCACCGTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCATgc                                                                           >  5:330151/1‑135 (MQ=255)                                                      gCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTgc                                                                           <  8:148251/135‑1 (MQ=255)                                                        acaTCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCCCAACGCCCGCTGCCCGCGCGGGGCTGCCCCCCGGGTGGCGGCCCCGTTGAGTACACAGACGCGGGTATAACGGCGCGCCCGCGTgcgc                                                                         >  5:9960/1‑135 (MQ=255)                                                             gCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCGTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCgcagc                                                                    >  3:18691/1‑135 (MQ=255)                                                               ccAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCCGGGTGCCGGCACCTTTTAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCAc                                                                  >  6:47429/1‑135 (MQ=255)                                                               ccAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCAc                                                                  >  3:127272/1‑135 (MQ=255)                                                               ccAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCAc                                                                  <  4:132835/135‑1 (MQ=255)                                                               ccAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCGCGCCCACCGCCCGCGGCCCGCTCGGGGCTGCCCCCCGGGGGCCGGCCCCGGTGAGGCCCCCGCCGCGGTGATAACGGCGCGCCCGCGTGCCCGCCGCAc                                                                  >  6:41546/1‑135 (MQ=255)                                                                cAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCCCCCGGGTGCCGGCCCCTTTGAGTACACAGACGCTGGTATAACGGCGCGCCCGCTTGCGCGCCGcccc                                                                 >  5:192785/1‑135 (MQ=255)                                                                    ccggcgccggcCCTCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCCCCCGGGTGCCGGCCCCGTTGAGTACACAGACGCTGTTTTAACGGCGCGCCCCCTTGCGCGCAGCCCCCggg                                                             >  6:291159/1‑135 (MQ=255)                                                                    ccggcgccggcCATCGCTGGGTGCCCGCGCTCGCACAACGACAGCTGCCCGCTCGGGGCTGCCACACGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCTCGCAAGCTTGCGCGCAGCACCAggg                                                             >  2:7385/1‑135 (MQ=255)                                                                     cggcgccggcCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGc                                                            <  2:126506/135‑1 (MQ=255)                                                                     cggcgccggcCATCGCTGGGGGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGc                                                            <  8:130052/135‑1 (MQ=255)                                                                        cgccggcCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGt                                                         <  6:454939/135‑1 (MQ=255)                                                                           cggcCCTCGCTGGGTGCCCGCGCTCGCACAACCCCAGCTGCCCGCGCGGGGCTGCCCCCAGGGGGCCGGCCCCGTTGAGTACACAGCCGCGGGTATAACGGCGCGCCCGCGTGCGCGCCGCCCCAGGGccggccg                                                      >  4:244508/1‑129 (MQ=255)                                                                           cggcCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGc                                                                                                                           >  2:117429/1‑66 (MQ=255)                                                                           cggcCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGc                                                                                                                           <  1:117429/66‑1 (MQ=255)                                                                           cggcCATCGCGGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCGGTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCCGGGCGGTccg                                                      >  1:318766/1‑133 (MQ=255)                                                                                aTCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGt                                                 <  5:256696/135‑1 (MQ=255)                                                                                aTCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGt                                                 >  6:451590/1‑135 (MQ=255)                                                                                     tGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCCCCCGGGTGCCGGCACCTTTGAGTACACAGACGCGGTTATAACGGCGCGCCCGCTTGCGCGCAGCACCCGGGCGGTCCGCCTGTACGGc                                            >  2:222734/1‑135 (MQ=255)                                                                                                  tCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCCCCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTCCGGCCTCAGGGGCAATg                               >  4:355135/1‑135 (MQ=255)                                                                                                             cAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGa                                                                                                      <  5:415013/53‑1 (MQ=255)                                                                                                             cAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGa                                                                                                      >  6:415013/1‑53 (MQ=255)                                                                                                             cAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAgccg                    >  8:418939/1‑135 (MQ=255)                                                                                                               gCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCGTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAgccgcg                  <  6:308345/135‑1 (MQ=255)                                                                                                                   cccGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCCGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAgccgcgccgc              >  8:71992/1‑135 (MQ=255)                                                                                                                      gcgcGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTc           <  4:307370/132‑1 (MQ=255)                                                                                                                      gcgcGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTc           <  2:376613/132‑1 (MQ=255)                                                                                                                      gCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTc           <  1:386481/135‑1 (MQ=255)                                                                                                                      gCTCGGGGCTGCCACCAGGGGGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTc           <  4:307372/135‑1 (MQ=255)                                                                                                                        tCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGa                                                                                                      <  6:143395/42‑1 (MQ=255)                                                                                                                        tCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGa                                                                                                      >  5:143395/1‑42 (MQ=255)                                                                                                                            gggtGCCACCGGGGGGCCGGCACCTTTGTGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  3:244508/132‑1 (MQ=255)                                                                                                                            ggcggcCACCCGGGGCCCGGAACCTTTGAGTACACCGACGCTGTTAAAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  1:222734/135‑1 (MQ=255)                                                                                                                            ggCTGCCACCCGGGTGCCGGCACCTGTGAGGCCACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCCGGGCGGGCAGCCTGTACGGCCGCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  5:41546/135‑1 (MQ=255)                                                                                                                            ggCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  3:78428/135‑1 (MQ=255)                                                                                                                            ggCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  3:447773/135‑1 (MQ=255)                                                                                                                            ggCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  3:312843/135‑1 (MQ=255)                                                                                                                            ggCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  7:241895/135‑1 (MQ=255)                                                                                                                            ggCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  3:190618/135‑1 (MQ=255)                                                                                                                            ggCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  4:18691/135‑1 (MQ=255)                                                                                                                            ggCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  4:242255/135‑1 (MQ=255)                                                                                                                            ggCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  5:47429/135‑1 (MQ=255)                                                                                                                            ggCTGCCACCAGGGTGCCGGCACCGTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  3:103254/135‑1 (MQ=255)                                                                                                                            ggCTGCCACCAGGGGGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCgg     <  4:270154/135‑1 (MQ=255)                                                                                                                              cTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCGGGt   <  4:304068/135‑1 (MQ=255)                                                                                                                               tGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCGGGTg  <  8:294462/135‑1 (MQ=255)                                                                                                                               tGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCGGGTg  <  2:1965/135‑1 (MQ=255)                                                                                                                                      |                                                                                                                                TGCGCCCCAGGCATCCAGGCCTTGGGCCAACAGGGCTGCCAGCACGCCAGTCAGCACATCGCCCAGGCCGGCGCCGGCCATCGCTGGGTGCCCGCGCTCGCACAACGCCAGCTGCCCGCTCGGGGCTGCCACCAGGGTGCCGGCACCTTTGAGTACACAGACGCTGTTATAACGGCGCGCCAGCTTGCGCGCAGCACCAGGGCGGTCAGCCTGTACGGCCTCAGTGGCAATGCCCAGCAGCCGCGCCGCCTCCCCCGGGTG  >  ACB122/5562170‑5562430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑