Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,684,640 T→G 33.1% P120P (CCT→CCG)  CJ019_05336 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,684,6400TG33.1% 47.5 / 4.9 53P120P (CCT→CCG) CJ019_05336hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (11/19);  new base G (12/4);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 2.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCC  >  ACB122/5684512‑5684769
                                                                                                                                |                                                                                                                                  
cAGCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCTccccc                                                                                                                               >  4:157142/1‑134 (MQ=255)
  gCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCGccccccc                                                                                                                             >  4:224989/1‑132 (MQ=255)
  gCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCGCCCCCGa                                                                                                                             >  4:406342/1‑134 (MQ=255)
  gCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCGcccccca                                                                                                                             >  1:31486/1‑132 (MQ=255)
    aaGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGaaaa                                                                                                                          <  7:259558/135‑1 (MQ=255)
    aaGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGaaaa                                                                                                                          <  7:312088/135‑1 (MQ=255)
     aGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTGCCCCCCCCGCCCCCGcaaaa                                                                                                                         >  5:430152/1‑135 (MQ=255)
      ggCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCCCCGCCTGCGGCCCGGCCCGAGGTGGTCCCCCCCCCGCCCCCGCAAAAg                                                                                                                        >  3:101204/1‑135 (MQ=255)
       gCAGCGGCACCCTGGACAAGACAGCGGGGCCCAAGACCACTGAGCTGGCGCCGTGCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGt                                                                                                                       <  5:338716/135‑1 (MQ=255)
       gCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAATATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGt                                                                                                                       <  8:386998/135‑1 (MQ=255)
              cACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCTCCCCCGAAAAAGTCGGCAgt                                                                                                                >  1:14953/1‑135 (MQ=255)
              cACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCCCCCCCCCAAAACTCGGccct                                                                                                                >  6:156262/1‑132 (MQ=255)
                      aaaaGAACGCGTTGCCCAAGACCCCTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCCCCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTgac                                                                                                          <  2:128503/131‑1 (MQ=255)
                        aaGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCTCCCCCGCAAAAGACCGCAGTGGTGacg‑gcg                                                                                                     >  1:455810/1‑135 (MQ=255)
                            ttgggttGCCCAAGCCCAGTTAGCGGGCGCCGTTCCTGCACAGCAAGTACAACAACATCACCCCCCCGCGTGGGGCCATGCCCGAGTTGGTCCAACCCCCGCCCCCTAAAAAGGCGGCAGTGGTGACG‑ACGgcgc                                                                                                 <  4:406705/129‑1 (MQ=255)
                              gCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCTCCCCCGAAAAAGTCGGCCGGGGGGACG‑ACGGCGccc                                                                                               >  8:201749/1‑135 (MQ=255)
                              gCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCGCCCCCCCAAAAGTCGGCAGGGGTGCCG‑ACGGCGccc                                                                                               >  6:80201/1‑135 (MQ=255)
                                ggTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCCCCCCCCCAAAAATCGGGAGGGGTGGCG‑ACCGCGccccc                                                                                             >  3:226777/1‑133 (MQ=255)
                                  tGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCCGGCCCGAGGTGGTCCCACCCCCGCCCCCCCAAAACGAGGCCGGGGTGACG‑ACGGCcccccccc                                                                                           >  7:126894/1‑131 (MQ=255)
                                  tGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGGCGGCAGGGGTGACG‑ACGGCGCCCAAGc                                                                                           >  8:165493/1‑135 (MQ=255)
                                  tGCCCAAGACCACTGAGCTGGCGCCGTTACAGGACAGCAAGATCAACAAGATCAACACACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCCCCCACGAAAAACTCGGCAATTGTGACC‑AAGGCGCCCaatc                                                                                           >  4:416093/1‑133 (MQ=255)
                                    cccAACACCACTGCGCTGGCGCCGTACCAGCACAGCAACATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCg                                                                                         <  5:294101/135‑1 (MQ=255)
                                     ccAAGACCACTGCGCTGGCGCCGTTCCAGGCCACCAAGATCCACACGATCACCCCCCCCACTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGc                                                                                        <  1:104348/135‑1 (MQ=255)
                                     ccAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCACCCCCGAAAAAGTAGGCCGTGGTGACG‑CAGGCGCCCCACCCGc                                                                                        >  4:335241/1‑135 (MQ=255)
                                                 cgcgggCGCCGTGCCAGGACAGCAAGATCAACAAGACCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGc                                                                            <  8:185387/131‑1 (MQ=255)
                                                 aGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCCGGCCCGAGGTGGTCCCACCACCGCCCCCCAAAAAGTCGGGAGTGGTGCCG‑ACCGCGCCCAAGCCGCCCAAGGgcgcgc                                                                            >  7:72784/1‑135 (MQ=255)
                                                 aGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCTCCCCCGCAAAAGTCGGCAGTGGTGTCG‑ACGGCGCCCCAGCCCCCCAAGGGCGAGc                                                                            >  5:349789/1‑135 (MQ=255)
                                                 aGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCACCCCCGCAAAAGTCGGCAGGGGGGACG‑CCGGCGCCCCCTCCGCCCAACGTCGAGc                                                                            >  6:81114/1‑135 (MQ=255)
                                                  gcgggCGCCGTTCCAGGCCAGCAAGATCAACAAGATCAGACCCCCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGc                                                                            <  6:68905/131‑1 (MQ=255)
                                                      gcgcCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCaaa                                                                       <  5:156262/135‑1 (MQ=255)
                                                         ccGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCGCCCCCGCAAAAGTCGGCCGGGGGGGCG‑CCGGCGCCCCAGCCGCCCAAGGGCGCGcccccacc                                                                     >  7:60539/1‑129 (MQ=255)
                                                         ccGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCGCCCCCCCAAAAATCGGCAGTGGTGCCG‑ACCGCGCCCAACCCGCCCAACGTCGCACCCCAAccc                                                                    >  8:166372/1‑135 (MQ=255)
                                                           gTTCCAGGACAGCAAGATCAACACGATCACCCCACCGCCTGCGGCCAGGCCCGCGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCaa                                                                  <  7:276558/135‑1 (MQ=255)
                                                                         aGATCAACAAGATCACCCCCCCGCCTGCGGCCAGGCCCGAGGTGGTCACCCCCCCAACCCCGAAAAAGACCGCACGGGGGGCGCCCGCCGCCCACCCCCCACAA‑GACGAGCACAACACCAAGGAAACCAAGgcgc                                                    >  2:63992/1‑135 (MQ=255)
                                                                          gATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCGCCCCCGAAAAAGTCGGGAGTGGTTACG‑ACGGCGCCCAAGCCCCACCACGGCCCGCCCCAAACCAAGGAAAGCAACGCGCa                                                   >  5:613/1‑135 (MQ=255)
                                                                          gATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCCCCCCCACCCCCCCAAAAATGCGCACTGGGGACC‑CCCGCGCCCCACCCCCCCAAAGTCGACCCCCAACCCAACCAAACCAAgccccc                                                   >  8:464218/1‑130 (MQ=255)
                                                                          gATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCa                                                   <  4:169031/135‑1 (MQ=255)
                                                                          gATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCCCAAAATACGGCAGTGGTGACG‑ACCGCGCCCAAGCCCCACAACGGCCCGCCCCAACCCAACGAAAGCAACGCCCa                                                   >  1:289401/1‑135 (MQ=255)
                                                                               acaaGATCACCCCCCCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGGGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCa                                              <  8:126894/135‑1 (MQ=255)
                                                                                   gATCACCCCACCGCCTCCGGCCAGGCCCGCGGGGGTCCCACCCCCGCCCCCGAAAAAGTCGGCAGGGGGGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAagcc                                          <  4:340666/135‑1 (MQ=255)
                                                                                        ccccACCGCCTCCGGCCAGGCCCGAGGTGTGCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCcg                                     <  4:124303/135‑1 (MQ=255)
                                                                                              cGCCTGCGCCCAGGCCCCCGTTGGCCCCACCCCCGCCCCCGAAAAAGTCGGCAGGGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGa                               <  2:276902/135‑1 (MQ=255)
                                                                                                      gCCAGGCCCGAGGTGGTCCCCCCCCCTCCCCCGAAAAAGTAGGCAGTGGGGGCG‑ACGGCGCCCCAGCCGCCCAAGGTCGAGCCCCAACCCAAGGACAGCCAGGCGCCGCCCCAGCCCGCCCCGCCCCCGCCggcc                       >  8:388427/1‑133 (MQ=255)
                                                                                                      gCCAGGCCCGAGGTGGTCCCACCCCCGCCCCCGCAAAAGTCGGCACTGGTGGCG‑CCGGCGCCCCAGGCGCACAAGGTCGAGCCCCAACCCCAGGTACGCCAGGGGCCGCCCACGCCCGCCGCGCCGCCGCCgggc                       >  8:463490/1‑133 (MQ=255)
                                                                                                        ccggCCCGAGGTGGCCCCACCCCCGCCCCCCAAAACGGCGGCCGGGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACtt                     <  6:281080/133‑1 (MQ=255)
                                                                                                        cAGGCCCGAGGTTGTCCCACCCCCTCCCCCGAAAACGTCGGCAGTGGGGACG‑ACGGCGCCCAAGCCGCACAAGGGCGAGCCCAAACCCAAGGAAAGCAAGCCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACtt                     <  4:326044/135‑1 (MQ=255)
                                                                                                        cAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGGGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACtt                     <  6:111024/135‑1 (MQ=255)
                                                                                                             ccGAGGTGGTCCCCCCCCCTCCCCCGAAAAAGTCGGCCGGGGGGACG‑ACGGCGGCCCAGCCGCACAAGGGCGCGCCCCAACCCCAGGAAAGCAAGGCGCCGCCCAAGCCACTCGCGCCGCCGCCGGCCGTCGACa                >  8:240302/1‑135 (MQ=255)
                                                                                                                  gtggtCCCACCCCCTCCCCCGAACAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGGCGCGCCGACGCCGGACTTCGACAGCTCg           <  4:237911/135‑1 (MQ=255)
                                                                                                                  gtggtCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCg           <  8:60539/135‑1 (MQ=255)
                                                                                                                        ccccccccTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGCGCCCAAACCCAAGGAAAGCCAGGCGCCGCCCCAGCCCGCCGCGCCGCCGCCGGCCTTCGACAGCGcgccgcgg     >  7:205552/4‑133 (MQ=255)
                                                                                                                        ccACCCCCTCCCCCCAAAAAGTAGGCAGTGGTGACG‑CCGGCGCCCAAGCCCCCCAATGTCGCGCCCAAACCCAAGGAAAGCAACGGGCCGCCCAAGCCACCCGCGCCGCCGCCGGACTTAGCCACCGCGCAGCTg     >  4:141958/1‑135 (MQ=255)
                                                                                                                           cccccTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTcc  <  3:72678/135‑1 (MQ=255)
                                                                                                                                |                                                                                                                                  
CAGCAAGGCAGCGGCACCCTGGACAAGAAAGCGGTGCCCAAGACCACTGAGCTGGCGCCGTTCCAGGACAGCAAGATCAACAAGATCACCCCACCGCCTGCGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACG‑ACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGCCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCC  >  ACB122/5684512‑5684769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑